Effects of a new the retinoic acid derivatives ATPR on proliferation and differentiation of SKOV3 tumor cell

Abstract

Abstract: AIM: To explore the effect of the 4-amino-2-trifluoromethyl-phenyl retinate(ATPR) on the proliferation and differentiation of SKOV3 tumor cell in vitro.METHODS: Cell proliferation was assessed by MTT assay and growth curve of cells; morphologic changes were observed after Giemsa staining using inverted phase contrast microscope; CA125 were measured by ELISA; the cell cycle were analyzed by Flow Cytometry (FCM).RESULTS: The growth of SKOV3 cells was inhibited in a does-dependent manner after the treatment with ATPR. The phanero depressant effect appeared at 48 h, but it was significant after 72 h. Cell morphous was observed to be mature in inverted microscope. The content of CA125 decreased. The proportion of cells in G₀/G₁ phase increased while the cells in S phase decreased. Cell cycle progression was blocked in the G₀/G₁ phase.CONCLUSION: ATPR could inhibit the proliferation and induce differentiation in some degree. The direction of differentiation is still need further study.

Key words: ATPR, SKOV3 cell line, Proliferation, Induced differentiation

CLC Number:

R965.2

WU Fan-rong, HONG Fan-qing, CHEN Fei-hu. Effects of a new the retinoic acid derivatives ATPR on proliferation and differentiation of SKOV3 tumor cell[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(3): 247-251.

References

[1] Shen J, Shi JB, Chen FH, et al. Synthesis and anti-tumor activity of all-trans retinoic acid derivatives[J]. Chin Chem Let, 2009, 20(7): 809-811.
[2] 齐元麟, 张明芳, 赵蓉, 等. 眼镜蛇毒L-氨基酸氧化酶诱导人胃癌细胞MGC-803细胞凋亡的实验研究[J]. 中国临床药理学与治疗学, 2012, 17(2): 121-125.
[3] 赵俊刚, 任开明, 汤隽, 等. 维甲酸联合低浓度化疗药物对肺腺癌A549细胞的作用[J]. 中国医科大学学报, 2012, 41(6): 511-513.
[4] 王渝东. 全反式维甲酸对小细胞肺癌细胞的分化诱导作用研究[J]. 中国医药指南, 2008, 6(14): 33-34.
[5] 刘萍, 边强. 异维甲酸的不良反应[J]. 药学进展, 2004:2(3): 186-187.
[6] Garattini E, Paroni G, Terao M. Retinoids and breast cancer: new clues to increase their activity and selectivity[J]. Breast Cancer Res, 2012, 14(5): 111-112
[7] Kelsey L, Katoch P, Johnson KE, et al. Retinoids regulate the formation and degradation of gap junctions in androgen-responsive human prostate cancer cells[J]. PLoS One, 2012, 7(4): 32-46.
[8] Zhang D, Holmes WF, Wu S, et al. Retinoids and ovarian cancer[J]. Cell Physial, 2000, 185(1): 1-20.
[9] Kim Y, Lin Q, Glazer PM, et al. Hypoxic tumor microenvironment and cancer cell differentiation[J]. Curr Mol Med, 2009, 9(4): 425-434.
[10] 郭霞, 郭昱. 黄芩甙体外对人肝癌细胞BEL-7402的诱导分化作用[J]. 世界华人消化杂志, 2008, 16(10): 1119-1123.
[11] Arab M, Yaseri M, Ashrafganjoi T, et al. Comparison of two ovarian malignancy prediction models based on age sonographic findings and serum ca125 measurement[J]. Asian Pac J Cancer Prev, 2012, 13(8): 4199-4202.
[12] Li CW, Chu YH, Chen BS. Construction and clarification of dynamic gene regulatory network of cancer cell cycle via microarray data[J]. Cancer Inform, 2007, 18(2): 223-241.
[13] Bindels EM, Lallemand F, Balkenende A, et al. Involvement of G₁/S cyclins in estrogen-independent proliferation of estrogen receptor-positive breast cancer cells[J]. Oncogene, 2002, 21(53): 8158-8165.

[1]	FAN Jie, JIN Yongming, JIANG Xiaolong, JIANG Guohua. Molecular mechanism of lncRNA HOTAIR regulating miR-206 on the proliferation and apoptosis of rheumatoid arthritis synovial cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(7): 736-742.
[2]	WANG Yan, ZHANG Jinhui, ZHAO Yongchen, LIU Hongxiang, LIU Yawei, MIAO Huanhuan, YANG Xincai. Sequoiaflavone inhibits stem cell properties such as proliferation and invasion of gastric cancer cells by down-regulating PI3K/AKT signaling pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(5): 508-513.
[3]	DING Jiamin, WANG Youdi, ZHAO Xiali, JIANG Genfeng, CHEN Siwen, HONG Chengcheng, LI Shuqin, HU Weihua. Study on the treatment of thin endometrium with growth hormone and vitamin E [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(8): 857-862.
[4]	WANG Xinli, XU Xiaqing, FANG Hanbing, GUO Yuzhong. Study of TPA on enhancing the anti-tumor effects of cisplatin and reducing its renal toxicity [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(5): 535-543.
[5]	LI Yanyan, FANG Xiaojie, YIN Xin, SUN Xi, RAO Chunhui. circZNF124 regulates the proliferation, migration and invasion of colorectal cancer SW620 cells by targeting miR-4262 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1231-1239.
[6]	LEI Jing, HE Ping, CAI Wenlin, ZHANG Yongzhe, ZHANG Beiru, LIU Dajun. Cordyceps sinensis extracts attenuates HBx-induced mesangial cell proliferation and extracellular matrix synthesis by suppression of the PI3K/Akt pathways [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1247-1254.
[7]	CHEN Linjun, CHEN Wenbin. Jolkinolide B inhibits the proliferation and metastasis of colon cancer cells via blocking PI3K/Akt/NF-κB pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(3): 250-257.
[8]	PENG Fusheng, HUANG Xiaohui, LI Peng, TANG Jian'er. miR-34a inhibits proliferation of prostate cancer LNCaP cells by regulating androgen receptor gene [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(1): 10-17.
[9]	WANG Xianghai, XING Min, LYU Tangfeng, LIU Hongbing, SONG Yong. Effects of knocking down long intergenic non-coding RNA 00467 on the prognosis of patients with lung adenocarcinoma and its mechanism [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(8): 841-849.
[10]	HOU Bin, HUANG Weiping, YIN Zhongpu, HU Shousen. lncRNA PCAT19 inhibits the proliferation and invasion of nasopharyngeal carcinoma cells by adsorbing miR-142-5p and regulating the expression of ING3 gene [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(7): 721-727.
[11]	OU Jun, HOU Wenwen, TANG Jingwen, LI Jiaping, XU Qingyang. miR-106a regulates the proliferation of ovarian granulosa cells by targeting TIMP-2 [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(5): 527-532.
[12]	HUANG Kang, LI Ling, YE Qifa, PENG Guizhu. Expression of long non-coding RNA LINC00844 in hepatocellular carcinoma and its inhibiting effect on cell proliferation and migration [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(4): 366-372.
[13]	SONG Hui, WANG Yinghao. Resveratrol inhibits the proliferation of retinoblastoma cells via miR-937/FOXQ1 pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(2): 174-181.
[14]	CHENG Jianping, LI Zhen, ZHAO Xiaolin, YANG Mengyuan, JI Xiwei, YU Jiufei. Research on the expression of miR-186-5p in serum of patients with colon cancer#br# [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(10): 1097-1104.
[15]	ZHANG Kang, HE Binjun, HU Wenbin, HAN Xiaoliang. Silencing carbonic anhydrase 1 inhibits the proliferation, invasion and migration of A549 cells in lung cancer [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(1): 55-60.