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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2013, Vol. 18 ›› Issue (4): 394-398.

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Inhibitory effects of puerarin on contractile activity of isolated gastrointestinal smooth muscle in rats and the related mechanisms

ZHANG Li-xue1,2, LI Hong-fang1,2,3, JIN Shan1, WANG Long-de4, MA Qin1,2, DOU Zhong-rui1,2, HUANG Jin-bing1,2   

  1. 1 Department of Physiology, College of Basic Medicine, Lanzhou University, Lanzhou 730000, Gansu, China;
    2 Function Laboratory in College of Basic Medicine, Lanzhou University, Lanzhou 730000, Gansu, China;
    3 Key Laboratory of Gansu Province Pre-clinical, Lanzhou 730000, Gansu, China;
    4 Affiliated Hospital of College of Traditional Chinese Medicine, Lanzhou 730000, Gansu, China
  • Received:2012-09-18 Online:2013-04-26 Published:2013-04-26

Abstract: AIM: To investigate the effects of Puerarin (Pue) on contractile activity of Gastrointestinal smooth muscle of rats and to explore its underlying mechanisms.METHODS: Gastrointestinal smooth muscle strips were taken out from rats. Each isolated muscle strip was put in a tissue chamber contains 5 mL Krebs solution and the motility of muscle strips was recorded simultaneously.RESULTS: Puerarin could reduce the resting tension and mean contractile amplitude of gastrointestinal smooth muscle strips, which were dose dependent. To gastric body contraction amplitude for index, with Logistic method for calculating IC50 was 82.590 μmol/L(r=0.498). Phentolamine, L-NG-nitroarginine, Glibenclamide partially blocked the inhibition of puerarin on the contractile activity of the rat gastric body. IC50 was 222.408 μmol/L(r=0.495), 166.945 μmol/L(r=0.446), 149.902 μmol/L(r=0.596), respectively.CONCLUSION: Puerarin has inhibitory action on the isolated gastrointestinal smooth muscle strips of rats, which is partially mediated via adrenergic α receptor and related to NO pathway and potassium channel.

Key words: Puerarin, Gastrointestinal smooth muscle, Phentolamine, L-NG-nitroarginine, Glibenclamide

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