Regulation of Shenqiwan on the expression of aquaporin-2 in LoVo ceus

Abstract

Abstract: AIM: To elucidate the regulation of Shenqiwan on the altered expression and mRNA level of AQP2.METHODS: 30 normal male adult SD rats were stochastically allocated into 3 groups, respectively administrated with distilled water (the control group), low dose of Shenqiwan (1.5 g/kg) and high dose of Shenqiwan (3.0 g/kg), consecutively for 7 days. Rat serum containing constituents from Shenqiwan was prepared by cardiac blood sampling followed by centrifugation. Cell survival rates were analyzed by MTT assay for the appropriate dilution factor of medicated serum after 24 h exposure. Indirect immunofluorescence labeling visualized the distribution of AQP2. The expression of AQP2 was determined by Western blot and the mRNA level of AQP2 was detected by semi-quantitative RT-PCR.RESULTS: Both low dose of Shenqiwan medicated serum (at 50% concentration) and high dose (at 25% concentration) significantly up-regulate the expression of AQP2 (P<0.01) in comparison with the normal rat serum. Specifically high dose of Shenqiwan at 25% concentration further increases the expression of AQP2 (P<0.05). In addition, these two groups mentioned above obviously elevates the mRNA level of AQP2 (P<0.05).CONCLUSION: Shenqiwan-containing medicated serum may function in the genetic transcription and protein translation, hoisting up the gene and protein level of AQP2, which indicates Shenqiwan associated with the regulation of aquaporin for water metabolism.

Key words: Shenqiwan, Aquaporin2, Water metabolism, Serum pharmacology

CLC Number:

R965.2

JIN Rong-jia, LI Shou-ye, YANG Yuan-xiao, LI Chang-yu. Regulation of Shenqiwan on the expression of aquaporin-2 in LoVo ceus[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(5): 481-486.

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