Abstract: AIM: To establish the HPLC method for determination of cefprozil in plasma.METHODS: High-performance liquid chromatography with UV detection was used, Hypersil BDS C₁₈ (200 mm×4.6 mm, 5 μm) was the analysis column. The mobile phase was 20 mmol/L ammonium acetate/10% acetic acid(pH=3.7):acetonitrile=92∶8(V/V), the flow rate was 1.0 mL/min, the column temperature was 30 ℃, the detection wavelength was 282 nm,the injection volume was 20 μL.The plasma sample were directly precipitated with trifluoroacetic acid, the transfer supernatant was analyzed after centrifugation.RESULTS: Cefprozil was linear within 0.10-10.01 μg/mL(r=1.0000). The lowest quantitative concentration was 0.10 μg/mL.The RSD of intra-day or inter-day were less than 15%(n=15).CONCLUSION: The HPLC method is rapid, simple, convienient and effcient, and suitable for the determination of cefprozil in plasma.

Key words: Cefprozil, HPLC, Plasma concentration