Effect of Chonglou Shenghua Tang on the expression of MMP-9 and TIMP-1 in endometrium and blood leukocyte and C-reactive protein level after caesarian section

Abstract

Abstract: AIM: To detect the expression of matrix metalloproteinases-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in endometrium after caesarian section, and the change of blood leukocyte count, neutrophil percentage, serum C-reactive protein (CRP) level, and to explore the mechanism of Chonglou Shenghua Tang for preventing lochiorrhagia after caesarian section (CS).METHODS: The prospective study was designed as randomized and single blind research from Jan 2011 to Jan 2013. 60 women underwent caesarian section (CS) indicated by obstetric factors were enrolled from the 2ED clinical medical college of Zhejiang Chinese Medical University and assigned into three groups: group of Chonglou Shenghua Tang: 20 cases were administered by Chonglou Shenghua Tang 100 mL, per 12 h 14 d after CS; group of Classical Shenghua Tang: 20 cases were administered by Classical Shenghua Tang 100 mL, per 12 h 14 d after CS and group of placebo: 20 cases were administered by placebo 100 mL, per 12 h 14 d after CS. The expressions of MMP-9 and TIMP-1 in endometrium were determined using immunohistochemistry at 16th days after CS, and blood leukocyte and CRP were detected at 5th days and 16th days after CS.RESULTS: The levels of MMP-9 expression decreased gradually in Chonglou Shenghua Tang group, Classical Shenghua Tang group and placebo group at 16th days after CS, respectively, with statistical significance (P＜0.05). The levels of TIMP-9 expression and the ratio of MMP-9/TIMP-1 increased gradually in Chonglou Shenghua Tang group, Classical Shenghua Tang group and placebo group at 16th days after CS,respectively, with statistical significance (P＜0.05). Blood leukocyte count, neutrophil percentage and CRP increased gradually in Chonglou Shenghua Tang group, Classical Shenghua Tang group and placebo group at 5th days and 16th days after CS, respectively, with statistical significance (P＜0.05).CONCLUSION: These results suggest that reduced expressions of MMP-9, and increased TIMP-1,and reduced the ratio MMP-9/TIMP-1 in endometrium and reduced inflammatory indexes in the blood may play important roles in Chonglou Shenghua Tang preventing lochia after cesarean section.

Key words: Chonglou Shenghua Tang, matrix metalliproteinases, blood leukocyte, C-reactive protein, caesarian section, lochiorrhagia

CLC Number:

R714.4

JIANG Yan-jiao, YE Hui-jun, LIU Jia-li, ZHAO Zai-qiu, SUN Kai. Effect of Chonglou Shenghua Tang on the expression of MMP-9 and TIMP-1 in endometrium and blood leukocyte and C-reactive protein level after caesarian section[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(5): 542-547.

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