Effect of bufalin on the proliferation and apoptosis of human bladder cancer T24 cells

Abstract

Abstract: AIM: To investigate the effect of bufalin on the proliferation of human bladder cancer T24 cells and explore the underlying anticancer mechanisms of bufalin. METHODS: The proliferation-inhibiting effect of bufalin on T24 cells was measured by methyl thiazole tetrazolium (MTT)colorimetric assay; the cell cycle distribution and apoptosis were detected by flow cytometry ( FCM ); the expression of CyclinD1 was detected by RT-PCR.RESULTS:Bufalin inhibited the proliferation of T24 cells in a time- and dose-dependent manner; bufalin could induce apoptosis of T24 cell; exposure of T24 cells to 20 nmol/L of bufalin led to significant induction of G₀/G₁ growth arrest at both 12 h (69.76%) and 24 h (88.14%),compared with negative control cells (36.42%).The mRNA levels of CyclinD1 in T24 cells was markedly decreased after treatment with bufalin.CONCLUSION: Bufalin could inhibit the proliferation and induce apoptosis of T24 cells. Bufalin could induce T24 G₀/G₁ growth arrest and decrease the mRNA levels of CyclinD1.

Key words: bladder cancer, bufalin, cell cycle, CyclinD1

CLC Number:

R965.2

HUANG Hou-bao, XIA Dong-dong, SHEN Qun-shan, CHEN Qing-shui, WANG Xiao, QIU Teng. Effect of bufalin on the proliferation and apoptosis of human bladder cancer T24 cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(7): 733-737.

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