Effects of platelet inhibitor from Agkistrodon halys venom on artery thrombosis in rabbit

Abstract

Abstract: AIM: To explore the effects and possible mechanism of platelet inhibitor from Agkistrodon halys venom (AHV-PI) on artery thrombosis in rabbit. METHODS: Thirty rabbits were divided into control group, artery thrombosis model group and AHV-PI (0.2 mg/kg) group (n=10) randomly. The model of rabbits carotid artery thrombosis was induced by infiltering 70%FeCl₃.The blood was taken from another carotid artery after 1 hour.Automatic coagulation analyzer was used to determined the APTT,PT,TT and the content of fibrinogen(FIB).The plasma content of plasmin was determined by Enzyme-Linked Immunosorbnent Assay (ELISA). RESULTS: Compared with control group, significantly shortened APTT, PT, TT and reduced plasmin level were observed in artery thrombosis model group (P<0.05), while the level of FIB in plasma was increased (P<0.05). Obviously prolonged APTT and TT, reduced FIB (P>0.05) and plasmin level were discovered in AHV-PI group as compared with model group, while PT had no difference (P<0.05).CONCLUSION: AHV-PI can prevent the artery thrombosis.The mechanism may be related to plasminogen activation.

Key words: Agkistrodon halys venom, artery thrombosis, plasminogen activator

CLC Number:

R965.2

JI Na, ZHANG Gen-bao, HUANG Lu, WANG Fei, ZHANG Ya, WU Juan. Effects of platelet inhibitor from Agkistrodon halys venom on artery thrombosis in rabbit[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(9): 987-990.

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