Abstract: AIM: To explore whether Agkistrodon Halys venom antitumor component-I (AHVAC-I) affects the migration of gastric cancer cells by human primary gastric cancer-associated fibroblast (GCAFs).  METHODS: Tissue block culture and trypsin digestion were used to separate and culture human primary gastric cancer-associated fibroblasts (GCAFs);  the GCAFs-CMAHVAC-I  was collected after AHVAC-I treatment to culture the MKN28 cells. Migration was evaluated by wound-healing and Transwell assay. ELISA and RT-PCR were used to verify wether AHVAC-I can play a role in the production of CXCL12 from GCAFs.RESULTS: AHVAC-I can decrease the CXCL12 production from GCAFs (P<0.05). Cultured with GCAFs-CMAHVAC-I , the migration of MKN28 cells were significantly inhibited (P<0.05). CONCLUSION: AHVAC-I can inhibit the migration ability of gastric cancer cells MKN-28 by decreasing CXCL12 production from GCAFs, and is valuable for gastric cancer therapy.

Key words: agkistrodon halys venom antitumor component-I, gastric cancer-associated fibroblast, gastric cancer cell migration, chemokine CXCL12