Correlation effect change of internal environmental in prostate antigen protein induced SD rat prostatitis

Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2014, Vol. 19 ›› Issue (9): 995-1000.

Correlation effect change of internal environmental in prostate antigen protein induced SD rat prostatitis

ZHOU Li¹, WANG Yong¹, LUO Yong-wei^{1, 2}, WANG Rong¹, MENG Xiang^{1, 2}, JIA Yu-ling^{1, 2}, LIU Xiang-yun¹, SUN Zu-yue¹

¹ Shanghai Institute of Planned Parenthood Research & National Evaluation Centre for Toxicology of Fertility Regulating Drugs, Shanghai 200032, China;
² School of Pharmacy, Fudan University, Shanghai 201203, China

Received:2014-01-01 Revised:2014-06-30 Online:2014-09-26 Published:2014-09-26

Abstract

Abstract: AIM: To explore the internal environment correlation prostate antigen protein induced SD male rats with chronic prostatitis. METHODS: 30 male rats were randomly divided into 3 groups, each one contained 10 ones. One was negative control group; the other two groups were the experimental group. 20, 40 mg/mL of rat prostate protein purification liquid combined with complete freund's adjuvant (1∶1 mixed suspension) were injected respectively in SD rats by multipoint intradermal injection at 0, 7 days and 0.5 mL of DPT vaccine was injected by intraperitoneal injection. At 21 days, 0.5 mL 20, 40 mg/mL of rat prostate protein purification liquid combined with complete freund's adjuvant were injected respectively in SD rats by intradermal injection. The negative control group was injected by equal dose of normal saline at the same time and place. RESULTS: Histopathological analyses indicated that there was inflammatory infiltrates in the experimental groups prostate stromal, which showed obvious chronic inflammation. Immune factors of chronic nonbacterial prostatitis can lead to IgG concentration increased significantly (P<0.05), CRP and PSA levels increased significantly (P<0.05). CONCLUSION: According to the experimental results, chronic non-bacterial prostatitis induced by prostate antigen protein lead to IgG concentration, CRP and PSA levels increased significantly.

Key words: autoimmunity prostatitis, chronic nonbacterial prostatitis, SD rat

CLC Number:

R965.1

ZHOU Li, WANG Yong, LUO Yong-wei, WANG Rong, MENG Xiang, JIA Yu-ling, LIU Xiang-yun, SUN Zu-yue. Correlation effect change of internal environmental in prostate antigen protein induced SD rat prostatitis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(9): 995-1000.

References 16

[1]	李科茂,王学本,赵如青. 慢性前列腺炎对男性生育的影响[J].中国性科学,2006,15(10):168-170.
[2]	Maccioni M, Rivero VE, Riera CM.Prostatein (or rat prostatic steroid binding protein) is a major autoantigen in experimental autoimmune prostatitis[J].Clin Exp Immunol, 1998,112(2):159-165.
[3]	谢琛静,周莉,李冬梅,等. 慢性非细菌性前列腺炎对大鼠生育力的影响[J]. 生殖与避孕, 2010,30(8):514-518.
[4]	李冬梅,胡文娟,刘向云,等. 大鼠自体免疫性前列腺炎模型的实验研究[J]. 实验动物与比较医学,2008,28(3):151-154.
[5]	Zhang ZY,Zug C,Schluesener HJ.Sphingosinc 1-phosphate receptor modulator FTY720 suppresses rat experimental autoimmune prostatitis[J].Scand J Immunol,2011,73:546-553.
[6]	闫晗,周莉,李冬梅,等. 普适泰片对SD大鼠慢性非细菌性前列腺炎作用的研究[J]. 中国临床药理学与治疗学, 2011,16(11):1234-1238.
[7]	Motrich RD, Maccioni M, Ponce AA, et al.Pathogenic consequences in semen quality of an autoimmune response against the prostate gland:from animal models to human disease[J]. J Immunol, 2006, 177(2):957-966.
[8]	李宏军. 前列腺炎的基本病因与发病机制[M]//郭应禄,李宏军,主编. 前列腺炎. 2版.北京:人民军医出版社,2007:81-107.
[9]	顾润国,王瑞东.前列腺炎对血清PSA水平的影响[J].中华男科学,2001,7(1):55-57.
[10]	Nadler RB, Collins MM, Propert KJ, et al.Prostate-specific antigen test in diagnostic evaluation of chronic prostatitis/ chronic pelvic pain syndrome[J]. Urology,2006,67(2):337-342.
[11]	Korrovits P, Ausmees K, Mndar R, et al.Seminal interleukin-6 and serum prostate-specific antigen as possible predictive biomarkers in asymptomatic inflammatory prostatitis[J]. Urology, 2011, 78(2): 442-446.
[12]	Hurlimann J, Thorbecke GJ, Hochwald GM, et al.The liver as the site of C-reactive protein formation[J]. J Exp Med,1996, 123(2): 365.
[13]	杨卫东. 外科手术中CRP检测应用的探讨[J]. 赣南医学院学报,2001,21(1):52-53.
[14]	梁景云,谢智光. C-反应蛋白在临床中的应用价值[J]. 检验医学与临床,2009,6(1):13.
[15]	储怡星. C-反应蛋白水平对判断炎症和创伤转归的价值[J]. 上海医学检验杂志,2000,15(3):155.
[16]	庄顺红,马拥军,马闪珊,等. 前列腺液锌含量与高敏C反应蛋白检测在前列腺炎中的应用价值[J]. 浙江检验医学,2009,24(2):137-138.