Glucagon-like peptide-1 and its analogue exendin-4 enhance proliferation and osteogenic differentiation of rat bone mesenchymal stem cells in vitro

Abstract

Abstract: AIM: To investigate the effects of GLP-1 and GLP-1R agonist exendin-4(EX-4) on the proliferation and differentiation of rat bone mesenchymal stem cells (BMSCs).METHODS: BMSCs were isolated from rats'tibia and femur by the method of density gradient centrifugation and attachment were passaged to the three generation. 4 groups were divided after 2 days cultivation, BMSC group, osteoblast induced medium(OIM),and drug induced medium. In the drug induced medium, different concentration of GLP-1 or EX-4 (10^-9-10^-7 mol/L) were added to the media of the BMSC for the observation of cell proliferation (OD₄₅₀),`differentiation (ALP staining) and mineralization (alizarin red staining for the observation of calcium nodule) of the BMSCs. RT-PCR was used to detect the expression of ALP, Runx2.RESULTS: Compared with the control group,GLP-1,EX-4 treatment groups promoted cells proliferation in a dose-dependent manner (P<0.05); the ALP and alizarin red staining suggested,GLP-1,EX-4 treatment groups were positive,the control group was weak stained or did not stain at all.ALP activity was improved, and the expressions of ALP and Runx2 were increased.CONCLUSION: The proliferation, differentiation and mineralization of the BMSCs are promoted directly by GLP-1 and EX-4, which may play an important role in bone reconstruction.

Key words: GLP-1, bone mesenchymal stem cells (BMSCs), osteogenesis, ALP

CLC Number:

R965.2

ZHOU Shi-meng, WANG Ning, MENG Jing-ru, JIA Min, HU Jing, MA Xue. Glucagon-like peptide-1 and its analogue exendin-4 enhance proliferation and osteogenic differentiation of rat bone mesenchymal stem cells in vitro[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(2): 121-127.

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