Research on the changes of protein C activity in plasma with myocardial ischemia reperfusion injury in rats and its mechanism

Abstract

Abstract: AIM: To investigate the active change of plasma protein C (PC), and its possible mechanism of myocardial ischemia-reperfusion injury in rats.METHODS: 50 SD male rats were randomly divided into control group (C) and ischemia reperfusion group (IR), then the rats of ischemia reperfusion group were randomly divided into ischemia 30 min(I₃₀), reperfusion 30(R₃₀), 60(R₆₀) and 120 (R₁₂₀)min group with 10 in each group. Ligated the rats' left anterior descending coronary artery 30 min then perfused to establish the model of ischemia reperfusion in vivo, monitored ECG changes in rat hart continuously by biological signal collection and processing system. Took carotid artery blood of the rats, to activated APTT, PT and TT; by chromomeric substrate method to tested PC activity; by enzyme linked immunosorbent assay (ELISA) to the content of Plasma free protein S (FPS); by turbidimetry to platelet aggregation rate; by a microscope to observe myocardial cells and interstitial organization.RESULTS: The ECG of ischemia reperfusion rats changed significantly and pathological observation showed disordered arrangement of myocardial cells, the nucleus pycnosis, interstitial edema; compared with control group, the plasma protein C activity decreased significantly (P<0.01) at ischemia 30 min, evidently picked up at early reperfusion, decreased again (P<0.01) at reperfusion 120 min; the content of plasma FPS apparently elevated at reperfusion 30 and 60 min (P<0.05), then was significantly down at reperfusion 120 min (P<0.05); platelet aggregation rate increased significantly (P<0.01) at ischemia 30 min, reperfusion 30 and 60 min; APTT significantly shortened after reperfusion 60 min (P<0.01). PT shortened after reperfusion 120 min (P<0.01), TT had not obviously change.CONCLUSION: The plasma protein C activity changed significantly during myocardial ischemia reperfusion and its mechanism may be related to the change of plasma FPS content and activation of platelets.

Key words: myocardial, ischemia reperfusion injury, protein C, platelet aggregation, protein S

CLC Number:

R965.2

ZHANG Ya, ZHANG Gen-bao, JI Na, WANG Fei, WU Juan. Research on the changes of protein C activity in plasma with myocardial ischemia reperfusion injury in rats and its mechanism[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(3): 284-288.

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