Effects of two types of schisandra preparations on the plasma concentrations of immunosuppressant tacrolimus in rats and protective effect of liver injury
LI Dong-liang, LI Bing, ZHANG Zhi-qiang, WANG Xiao, FANG Jian, ZHANG Shi-an, CAI Li-rong
2015, 20(3):
256-260.
Asbtract
(
411 )
PDF (959KB)
(
430
)
References |
Related Articles |
Metrics
AIM: To investigate the effects of two types of schisandra preparations,WZ capsules and bicyclol,on the plasma concentrations of immunosuppressant tacrolimus in rats with liver injury.METHODS: 55 rats were randomly divided into four groups: control group(n=10), the other three groups(n=15, in each case) only FK506 treatment group (1 mg/kg), FK506+WZ co-treatment group group(capsules 11.25 mg/kg), FK506+bicyclol combination group(bicyclol 200 mg/kg).once every day for 21 successive days,after 8 h fasting at 7,14,21 d the administration,the concentrations of FK506 and the liver function were detected. After the experiment the rats were killed,the changes of livers of rats in each group were observed.RESULTS: Plasma concentrations of FK506 in FK506 treatment group were increased slowly without schisandra preparations, the plasma concentrations of FK506 were increased in FK506+WZ co-treatment group and FK506+bicyclol combination group.The plasma concentrations of FK506 in FK506+WZ co-treatment group were increased fastly,compared with the control group, there was statistically significant difference in FK506+WZ co-treatment group,7 d after administration (F=11.043, P=0.002), 14 d (F=98.167, P=0.000) and 21 d (F=57.457, P=0.000). Compared with the FK506+WZ co-treatment group,there was no statistically significant difference in FK506+bicyclol combination group at each time point. Compared with the control group,bicyclol had liver protection obviously,the level of ALT was decreased 7 d after administration(F=12.83,P=0.001),14 d(F=11.58,P=0.002),21 d(F=22.27,P=0.000),there was statistically significant difference.The effect of on reducing ALT was weak and work slowly in FK506+WZ co-treatment group, which had no statistical significance,compared with the control group in 7 d,14 d,21 d after administration. Compared with control group,bicyclol group could keep ALB at a higher level,there was statistically significant difference at each time point(P<0.05). The damage of FK506 to hepatocyte occurred early after administration, histologically characterized by edema, degeneration of liver cells and inflammatory cell infiltration.CONCLUSION: The WZ capsules and high-dose of bicyclol can rapidly increase the plasma concentrations of FK506,the damage of FK506 to hepatocyte occurs early after administration. Bicyclol can prevent liver from the damage of FK506.