Effect of anti-tumor necrosis factor antibody on TRAIL and TNF-α expression induced by lipopolysaccharide in human periodontal ligament fibroblasts

Abstract

Abstract: AIM: To investigate the possible roles of tumor necrosis factor(TNF) superfamily in the pathogenesis of periodontal disease inflammation, the effect of anti-tumor necrosis factor antibody on TRAIL and TNF-α expression induced by lipopolysaccharide in human periodontal ligament fibroblasts is observed.METHODS: HPLFs were cultured to the sixth generation cells and were interfered with defferent concentrations of lipopolysaccharide(0,0.1,1,10,100 μg/mL,respectively) for 24 h, named group Z₀,Z_0.1,Z₁,Z₁₀ and Z₁₀₀. HPLFs were also cultured with 1 μg/mL lipopolysaccharide and 75 μg/mL anti- TNF antibody (named group Z₁+75). The expressions of both TNF-α and TRAIL mRNA were analysed by Real-time quantitative reverse transcription polymerase chain reaction(QRT-PCR),while TRAIL protein were determined by West Blot methods.RESULTS: The expressions of TRAIL mRNA in group Z₁ or Z₁₀ were significantly higher than those in group Z₀ or Z_0.1 (all P<0.05),in group Z₁₀₀ were significantly higher than those in group Z₀(P<0.05).But there were no significant differences neither between group Z₁ and Z₁₀ nor among group Z₁₀₀, Z_0.1, Z₁ and Z₁₀ (all P>0.05). Moreover, the expressions of TNF-α mRNA in group Z₁ or Z₁₀ were significantly higher than those in group Z₀ or Z₁₀₀ (all P<0.05), whereas there were nonsignificant differences neither between group Z₁ and Z₁₀ nor among group Z₀, Z_0.1, and Z₁₀₀ (all P>0.05). Dramatically, the expressions of TRAIL protein were significant difference between each groups, in group Z₁₀₀ <Z₀ < Z_0.1 <Z₁(all P<0.05 or P<0.01).And it was significantly higher in group Z₁₀ than those in group Z₀ or Z₁₀₀(all P<0.01),while there were nonsignificant differences neither between group Z₁₀ and Z_0.1 nor among group Z₁₀ and Z₁ (all P>0.05). Noteworthy, the expressions of either TRAIL mRNA and protein or TNF-α mRNA in group Z₁+75 were significantly decreased than those in group Z₁(P<0.05 or <0.01,respectively). Furthermore,there were significance positive correlation between levels of TRAIL and TNF-α mRNA(r=0.819,n=30, P<0.01).CONCLUSION: The results shows that the expressions of TRAIL and TNF-α in HPLFs can be induced by lipopolysaccharide, the levels of them were elevated in the first and decreased subsequently, this changes were accompanied by lipopolysaccharide concentration. Date also show the function of lipopolysaccharide can be inhibited by anti-TNF antibody.

Key words: human periodontal ligament fibroblasts, tumor necrosis factor, TNF-related apoptosis-inducing ligand, lipopolysaccharide, anti-tumor necrosis factor antibody, periodontal disease

CLC Number:

CHEN Yan, YE Bin, MOU Wen-yuan, YU Cheng-bo, LIN Bu, YIN Xiang-peng, XU Hong-miao. Effect of anti-tumor necrosis factor antibody on TRAIL and TNF-α expression induced by lipopolysaccharide in human periodontal ligament fibroblasts[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2015, 20(5): 493-498.

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