Prenatal ethanol exposure induces dysfunction of glucose metabolism in rats fed by high-fat diet

Abstract

Abstract:

AIM: To investigate the effects of prenatal ethanol exposure (PEE) on glucose metabolism in rats fed by high-fat diet. METHODS: The maternal Wistar rats were administered with ethanol (4 g·kg^-1·d^-1) from gestational day-11 until full-term parturition, while the control rats were given the same volume of normal saline. All pups were fed with high-fat diet after weaning until postnatal week (PW) 20. And they were subjected to oral glucose tolerance test (OGTT) at PW 20 and sacrificed at PW 24. The morphology of the pancreas was presented by Hematoxylin-Eosin staining, and mRNA expression of key genes of hepatic insulin signaling pathwaywas was measured by real-time PCR. RESULTS: Compared with the control offspring, the body weight of the PEE offspring rats was lower (P<0.01) at PW1, and the body gain rates increased significantly after birth (P<0.01); high-fat diet induced more severe damage in pancreas of the PEE rats; the basic serum insulin level of PEE rats was lower, the release of serum insulin increased after glucose loading, but the absolute insulin level was still lower than that of the control group; the mRNA expression of key genes inhepatic insulin signaling pathway increased significantly(P<0.01). CONCLUSION: PEE rats fed with high-fat diet present obvious catch-up growth pattern and decreased pancreatic glucose metabolism, while the insulin sensitivity increases, especially in the female rats.

Key words: glucose metabolism, high-fat diet, prenatal ethanol exposure

CLC Number:

R965.2

LI Xia, XIAO Di, KOU Hao, ZHANG Li, JI Zhenyu, GUO Yu, WANG Hui. Prenatal ethanol exposure induces dysfunction of glucose metabolism in rats fed by high-fat diet[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(1): 20-26.

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