Effect of baicalin on vitiligo mice induced by monobenzone

Abstract

Abstract:

AIM: To study the effect of baicalin on vitiligo mice induced by monobenzone. METHODS: 40 C57BL/6 mice were randomly divided into four groups (n=10), i.e. the negative control group, the model group, the halometasone group and 5% baicalin group. 40% monobenzone cream was applied on C57BL/6 mice to induce vitiligo model. 5% baicalin was used to observe its efficacy on vitiligo and possible mechanism. After treatment for 50 days, hair decolorizing was observed with naked eye, melanin and melanocytes were observed by reflectance confocal microscopy (RCM), and CD+8T cells were tested by immunofluorescence detection. 5 vitiligo-related genes (CXCL9, CXCL10, CXCR3, RAB27A, PI3K) were detected by real-time fluorescence quantitative PCR. RESULTS: Mice in model group showed depigmentation at the monobenzone application and non-application sites.The halometasone group and 5% baicalin group manifested less, delayed, smaller decolorization with fewer infiltrated lymphocytes and CD+8T cells compared with the model group. Expressions of CXCL9, CXCL10, CXCR3 and RAB27A detected by Fluorescence real-time quantitative PCR in the halometasone group and 5% baicalin group was significantly lower than that of the model group, while PI3K increased significantly. No significant difference was observed between the halometasone group and the 5% baicalin group, yet the halometasone group showed incidence of skin atrophy after 30 days' treatment. CONCLUSION: Halometasone and 5% baicalin are both effective for treating vitiligo, while baicalin presented with safer results, which can be referential for clinical application.

Key words: baicalin, monobenzone, vitiligo

CLC Number:

R965.2

ZHU Yiping, JIN Rong,WANG Suiquan, XU Ai'e. Effect of baicalin on vitiligo mice induced by monobenzone[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(1): 27-32.

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