Protective effect of sulfated pachymaran on MPTP-induced damage in mouse dopaminergic neurons

Abstract

Abstract:

AIM: To explore the protective effect of sulfated pachymaran (SP) on MPTP- induced damage in mouse substantia nigra neurons. METHODS: ICR mice were randomly divided into five groups: control group, MPTP model group and SP group (50, 100, 150 mg/kg), and all drugs were administrated to mice by abdominal injection. Pole test was performed to assess the ability of moving. TH immunostaining and TUNEL labeling were used to observe the damage and apoptosis of nigral neurons. The levels of dopamine (DA), malondialdehvde (MDA) and hydrogen peroxide (H2O2) in both midbrain and striatum were detected by UV spectrophotometry. RESULTS: Compared with the control group, the mice in MPTP model group took longer time to descend; TH-positive neurons and the level of DA in midbrain and striatum decreased obviously, and TUNEL-positive neurons increased significantly, but the level of H2O2 in midbrain and striatum did not vary significantly. Compared with the model group, SP treatment was shown to reverse all the above changes induced by MPTP. CONCLUSION: SP may have protective effects on MPTP-induced damage of dopaminergic neurons in Parkinsons' disease PD mice.

Key words: sulfated pachymaran, MPTP, apoptosis, dopamine, NOS

CLC Number:

R965.2

GAO Guizhen, WU Chao, XUE Hongyu, WANG Chao. Protective effect of sulfated pachymaran on MPTP-induced damage in mouse dopaminergic neurons[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(1): 37-42.

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