Inhibition effect and molecular mechanisms of cinnamaldehyde on the adhesion of polymorphonuclear cells to HUVECs

Abstract

Abstract:

AIM: To observe the inhibition effects and molecular mechanisms of cinnamaldehyde on the adhesion of polymorphonuclear(PMN) to human umbilical vein endothelial cells (HUVEC), and to explore the mechanism of cinnamaldehyde on promoting skin wound healing. METHODS: The effect of cinnamaldehyde on HUVEC and human peripheral PMN cell activity were observed by MTT and trypan-blue assay, repectively. The effect of cinnamaldehyde at high (10 mg/L), medium（5 mg/L）and low（2.5 mg/L）concentration on the adhesion of PMN to HUVEC primed by TNF were observed by Rose Bengal Stainning. Expressions of ICAM-1, VCAM-1 and CD44 on HUVEC were measured by fluorescent-immunocytochemistry assay. RESULTS: High dose of cinnamaldehyde decreased PMN-HUVEC adhesion at 4 h after treated by cinnamaldehyde and TNF (P<0.05), and showed lower level of VCAM-1 on HUVEC（P<0.01）. Medium dose of cinnamaldehyde decreased the level of ICAM-1 and VCAM-1 on HUVEC（P<0.05）. There was no statistic difference for cinnamaldehyde on the level of CD44 on HUVEC. CONCLUSION: Cinnamaldehyde may inhibit the adhesion of PMN to HUVEC by decreasing the expression of adhesion molecules on HUVEC, therefore inhibiting excessive inflammatory response.

Key words: wound healing, cinnamaldehyde, adhesion molecules, human umbilical vein endothelial cells (HUVEC)

CLC Number:

R965.2

HE Xiujuan, LIN Yan, MENG Yujiao, LI Ping. Inhibition effect and molecular mechanisms of cinnamaldehyde on the adhesion of polymorphonuclear cells to HUVECs[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(1): 43-47.

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