矢车菊素-3-葡萄糖苷对肥胖大鼠低度炎症反应及胰岛素敏感性的影响

doi:10.11852/zgetbjzz2014-22-08-13

摘要/Abstract

摘要： 目的观察矢车菊素-3-葡萄糖苷(cyanidin-3-glucoside, C3G)对肥胖大鼠血清炎症因子(TNF-α、IL-6和MCP-1)及胰岛素敏感性的影响。方法 3周龄雄性SD大鼠30只, 随机分为对照组(n=8)和高脂饮食组(n=22), 分别予以普通饲料及高脂饲料喂养5周。肥胖造模成功的17只大鼠再随机分为肥胖组(n=8)和C3G组(n=9)。C3G组予C3G 100 mg/(kg·d)灌胃, 其余组予等量生理盐水灌胃。实验结束时准确称量大鼠体重及内脏脂肪质量, 全自动生化分析仪测定空腹血糖(fasting glucose, FPG), 放免法测血清胰岛素(fasting insulin, FINS), ELISA测血清脂联素及TNF-α、IL-6、MCP-1水平, 并计算内脏脂肪比和胰岛素敏感指数(insulin sensitivity index, IAI)。结果肥胖组和C3G组大鼠体重、内脏脂肪比和血清TNF-α、IL-6、MCP-1水平明显高于对照组(P均<0.05), 而C3G组上述指标明显低于肥胖组(P均<0.05)。肥胖组和C3G组血清脂联素水平及IAI明显低于对照组(P均<0.05), 而C3G组上述指标明显高于肥胖组上述指标。结论 C3G能明显增加肥胖大鼠的胰岛素敏感性, 其可能与增加血清脂联素水平及降低血清炎症因子(TNF-α、IL-6、MCP-1)水平有关。

关键词: 矢车菊素-3-葡萄糖苷, 低度炎症, 胰岛素敏感性, 脂联素, 肥胖, 大鼠

Abstract: Objective To examine the effects of cyanidin-3-glucoside (C3G) on serum inflammatory cytokines and insulin sensitivity in diet-induced obese rats. Methods Thirty male Sprague-Dawley rats were randomly assigned to a control group (n=8) and a high fat diet group (n=22), and were fed with standard diet or high fat diet.Five weeks later, 17 high-fat diet-induced obese rats were assigned to a obese group (n=8) and a C3G group (n=9).Then, rats of obese group were treated with C3G at 100 mg/(kg·d) for 5 weeks.Body weight, visceral adiposity weight were measured, blood sample was collected for detecting levels of fasting glucose(FPG), fasting insulin(FINS), leptin, adiponectin, TNF-α, IL-6 and MCP-1, finally calculated visceral adiposity weight to body weight ratio(VBR) and insulin sensitivity index(IAI). Results Body weight, VBR, TNF-α, IL-6 and MCP-1 in the obese group were significantly higher than those of the control group (all P<0.05).The C3G group had significantly lower levels of body weight, VBR, TNF-α, IL-6 and MCP-1 than the obese group(all P<0.05), but the levels of the paremeters in the C3G group was significantly higher than that of the control group (P<0.05).Adiopnectin and insulin sensitivity index in the obese group was significantly lower than that of the control group (P<0.05), C3G treatment dramatically increased adiponectin levels and insulin sensitivity index compared with the obese group (P<0.05), although the levels of the two parameters did not reach to the levels of the control group. Conclusion Cyanidin-3-glucoside can increase insulin sensitivity through regulating serum inflammatory cytokines levels in obese rats.

Key words: cyanidin-3-glucoside, low-grade inflammation, insulin sensitivity, adiponectin, obesity, rats

中图分类号:

Q95-33

金辉, 马路一. 矢车菊素-3-葡萄糖苷对肥胖大鼠低度炎症反应及胰岛素敏感性的影响[J]. 中国儿童保健杂志, 2014, 22(8): 826-828.

JIN Hui, MA Lu-yi.. Effect of cyanidin-3-glucoside on low-grade inflammation and insulin sensitivity in diet-induced obese rats.[J]. journal1, 2014, 22(8): 826-828.

参考文献

[1] Swinburn BA, Sacks G, Hall KD, et al.The global obesity pandemic:shaped by global drivers and local environments[J].Lancet, 2011, 378(9793):804-814.
[2] Shoelson SE, Herrero L, Naaz A.Obesity, inflammation, and insulin resistance[J].Gastroen-terology, 2007, 132(6):2169-2180.
[3] Lê KA, Mahurkar S, Alderete TL, et al.Subcutaneous adipose tissue macrophage infiltration is associated with hepatic and visceral fat deposition, hyperinsulinemia, and stimulation of NF-κB stress pathway[J].Diabetes, 2011, 60(11):2802-2809.
[4] Farb MG, Bigornia S, Mott M, et al.Reduced adipose tissue inflammation represents an in-termediate cardiometabolic phenotype in obesity[J].J Am Coll Cardiol, 2011, 58(3):232-237.
[5] He J, Giusti MM.Anthocyanins:natural colorants with health-promoting properties[J].Annu Rev Food Sci Technol, 2010, 1:163-187.
[6] 汤锦花, 严海东.营养性肥胖大鼠模型的建立及评价[J].同济大学学报:医学版, 2010, 21(1):32-34.
[7] Chandler PC, Viana JB, Oswald KD, et al.Feeding response to melanocortin agonist predicts preference for and obesity from a high-fat diet[J].Physiol Behav, 2005, 85(2):221-230.
[8] Sun K, Kusminski CM, Scherer PE.Adipose tissue remodeling and obesity[J].J Clin Invest, 2011, 121(6):2094-2101.
[9] Suganami T, Tanaka M, Ogawa Y.Adipose tissue inflammation and ectopic lipid accumula-tion[J].Endocr J, 2012, 59(10):849-857.
[10] Spalding KL, Arner E, Westermark PO, et al.Dynamics of fat cell turnover in humans[J].Nature, 2008, 453(7196):783-787.
[11] Takikawa M, Inoue S, Horio F, et al.Dietary anthocyanin-rich bilberry extract ameliorates hyperglycemia and insulin sensitivity via activation of AMP-activated protein kinase in dia-betic mice[J].J Nutr, 2010, 140(3):527-533.
[12] Guo H, Xia M, Zou T.Cyanidin 3-glucoside attenuates obesity-associated insulin resistance and hepatic steatosis in high-fat diet-fed and db/db mice via the transcription factor FoxO1[J].J Nutr Biochem, 2012, 23(4):349-360.
[13] Deng T, Lyon CJ, Minze LJ, et al.Class II major histocompatibility complex plays an essen-tial role in obesity-induced adipose inflammation[J].Cell Metab, 2013, 17(3):411-422.
[14] Hruskova Z, Biswas SK.A new "immunological" role for adipocytes in obesity[J].Cell Me-tab, 2013, 17(3):315-317.
[15] Lumeng CN, DelProposto JB, Westcott DJ, et al.Phenotypic switching of adipose tissue ma-crophages with obesity is generated by spatiotemporal differences in macrophage subtypes[J].Diabetes, 2008, 57(12):3239-3246.
[16] Johnson AM, Olefsky JM.The origins and drivers of insulin resistance[J].Cell, 2013, 152(4):673-684.
[17] Coelho M, Oliveira T, Fernandes R.Biochemistry of adipose tissue:an endocrine organ[J].Arch Med Sci, 2013, 9(2):191-200.
[18] Awazawa M, Ueki K, Inabe K, et al.Adiponectin enhances insulin sensitivity by increasing hepatic IRS-2 expression via a macrophage-derived IL-6-dependent pathway[J].Cell Metab, 2011, 13(4):401-412.