核转录因子κB和小清蛋白阳性神经元在丙戊酸钠孤独症模型鼠海马CA1区的表达

doi:10.11852/zgetbjzz2014-22-12-12

中国儿童保健杂志 ›› 2014, Vol. 22 ›› Issue (12): 1271-1273.DOI: 10.11852/zgetbjzz2014-22-12-12

核转录因子κB和小清蛋白阳性神经元在丙戊酸钠孤独症模型鼠海马CA1区的表达

衣明纪¹,孙吉莲²,陈蕾³,李音⁴,冯雪英¹

1 青岛大学医学院附属医院儿童保健科,山东青岛 266003;
2 文登市妇幼保健院,山东威海 264400;
3 青岛大学医学院附属医院小儿内科,山东青岛 266003;
4 青岛市妇女儿童医院儿童保健科,山东青岛 266034

收稿日期:2014-01-14 发布日期:2014-12-10 出版日期:2014-12-10
作者简介:衣明纪(1966-),男,山东人,主任医师,硕士学位,主要研究方向为发育行为儿科。
基金资助:
青岛大学医学院附属医院2010年重点培育学科专项建设基金(2010-610)

Changes of nuclear factor-kappa B and parvalbumin positive neurons in hippocampal CA1 region in VPA autism rats.

YI Ming-ji¹,SUN Ji-lian²,CHEN Lei³,LI Yin⁴,FENG Xue-ying¹.

1 Department of Child Health Care,the Affiliated Hospital of Medical College,Qingdao University,Qingdao,Shandong 266003,China;
2 Wendeng Health Center for Women and Children,Weihai,Shandong 264400,China;
3 Department of Pediatrics,The Affiliated Hospital of Medical College,Qingdao University,Qingdao,Shandong 266003,China;
4 Department of Child Health Care,Qingdao Hospital for Women and Children,Qingdao,Shandong 266034,China

Received:2014-01-14 Online:2014-12-10 Published:2014-12-10

摘要/Abstract

摘要： 目的建立丙戊酸钠(valproic acid,VPA)孤独症动物模型,观察核转录因子κB和小清蛋白阳性神经元在VPA孤独症模型鼠海马CA1区的表达,探讨孤独症可能的发病机制,为完善其治疗方案提供理论依据。方法按Schneider方法制作VPA孤独症动物模型,采用免疫组化和图像分析技术检测孤独症模型鼠海马CA1区核转录因子κB和小清蛋白阳性神经元的表达。结果与正常对照组比较,孤独症模型组海马CA1区核转录因子κB阳性神经元数目增加(4.67±1.56 vs 3.00±1.54,t=2.639,P=0.015);海马CA1区小清蛋白表阳性神经元数目增加(5.33±0.99 vs 2.83±1.27,t=5.378,P=0.000)。结论孤独症的发病可能与海马CA1区核转录因子κB和小清蛋白的表达水平有关。

关键词: 孤独症谱系障碍, 核转录因子κB, 小清蛋白, 丙戌酸钠, 大鼠

Abstract: Objective To establish the animal model of autism and explore pathogenesis of autism by observation of changes of nuclear factor-kappa B (NF-κB) and parvalbumin(PV) positive neurons in hippocampal CA1 region and to provide theoretical evidence for the therapeutic schedule of autism. Methods Animal model of autism was obtained as Schneider showed.The immunohistochemistry methods and image analysis were used to examine the number of NF-κB,and PV positive neurons in hippocampal CA1 region in autism model rats and the normal rats. Results The number of NF-κB positive neurons in the hippocampal CA1 region of the autism model rats was more than that of the normal rats(4.67±1.56 vs 3.00±1.54,t=2.639,P=0.015).The number of PV positive neurons in the hippocampal CA1 region of the autism model rats was more than that of the normal rats (5.33±0.99 vs 2.83±1.27,t=5.378,P=0.000). Conclusion The occurrence of autism may be related to the changes of NF-κB and PV in hippocampal CA1 region.

Key words: autism spectrum disorder, nuclear factor-kappa B, parvalbumin, valproic acid, rats

中图分类号:

R749.94

衣明纪,孙吉莲,陈蕾,李音,冯雪英. 核转录因子κB和小清蛋白阳性神经元在丙戊酸钠孤独症模型鼠海马CA1区的表达[J]. 中国儿童保健杂志, 2014, 22(12): 1271-1273.

YI Ming-ji,SUN Ji-lian,CHEN Lei,LI Yin,FENG Xue-ying.. Changes of nuclear factor-kappa B and parvalbumin positive neurons in hippocampal CA1 region in VPA autism rats.[J]. journal1, 2014, 22(12): 1271-1273.

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核转录因子κB和小清蛋白阳性神经元在丙戊酸钠孤独症模型鼠海马CA1区的表达

Changes of nuclear factor-kappa B and parvalbumin positive neurons in hippocampal CA1 region in VPA autism rats.

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