关键词: 人脐带间充质干细胞, 缺氧缺血性脑损伤, 移植, 经颈静脉, 经腹腔

Abstract: Objectiv To compare the distribution and differentiation of human umbilical cord mesenchymal stem cells(hUCMSCs) after the transplantation via the jugular vein and via the intraperitioneal injection,and investigate the effect of hUCMSCs transplantation on the recovery of neurological functions after hypoxic-ischemic brain damage (HIBD). Methods The models of 7-day-old neonate rats with HIBD brain were established,and randomly assigned to:HIBD group (n=10),the intravenous injection group (n=15),the intraperitioneal injection group (n=15) and control group (n=10),the HIBD group and control group were not transplanted.The same amount hUCMSCs(1×10⁶,0.2 mL)were transplanted into the intravenous injection group and the intraperitioneal injection group after 72 hours.Finally,brain tissues of rats were removed after 4% paraformaldehyde was perfused in cardiac to fix brain and successive sections were made using a cryomicrotome.Immunofluorescence staining was performed to examine the distribution of Dil-labeled hUCMSCs and expression of DCX. Results The transplantation descendants of hUCMSCs could migrate to the brain tissue,and could differentiate into neuronal precursor cells,and the intravenous injection group showed more Dil-labeled hUCMSCs in brain tissue. Conclusions Transplanted hUCMSCs can survive and differentiate in the HIBD rats' brain.The HUCMSCs can effectively accelerate the recovery of neurological functions after HIBD.And the experiment proved intravenous injection is better than intraperitioneal injection in repairing rats' brain function.

Key words: human umbilical cord mesenchymal stem cells, hypoxic-ischemic brain damage, transplantation, intravenous injection, intraperitioneal injection