1型糖尿病儿童氧化应激水平的研究

doi:10.11852/zgetbjzz2017-25-07-08

中国儿童保健杂志 ›› 2017, Vol. 25 ›› Issue (7): 675-678.DOI: 10.11852/zgetbjzz2017-25-07-08

1型糖尿病儿童氧化应激水平的研究

赵永鑫¹, 辛颖²

1 大连市妇幼保健院儿科,辽宁大连 116083;
2 中国医科大学附属盛京医院儿科,辽宁沈阳 110004

收稿日期:2017-02-06 发布日期:2017-07-10 出版日期:2017-07-10
通讯作者: 辛颖:E-mail:xin.ying168@live.cn
作者简介:赵永鑫(1980-),女,山西人,主治医师,硕士研究生,主要研究方向为儿童内分泌疾病及儿童行为问题。

Status of oxidative stress in children with type 1 diabetes mellitus

ZHAO Yong-xin¹, XIN Ying²

1 Pediatric Department,Dalian Maternal and Child Health Care Hospital,Dalian,Liaoning 116033,China;
2 Pediatric Department,ShengJing Hospital of China Medical University,Shenyang,Liaoning 110004,China

Received:2017-02-06 Online:2017-07-10 Published:2017-07-10
Contact: XIN Ying,E-mail:xin.ying168@live.cn

摘要/Abstract

摘要： 目的比较1型糖尿病患儿与健康儿童血清中氧化应激标志物水平,探讨1型糖尿病患儿氧化及抗氧化能力的改变。方法选取62例1型糖尿病患儿为病例组,36例健康儿童为对照组。病例组根据病程分为初发组和病程稍长组;根据感染情况分为感染组和非感染组。测定各组血清中氧化标志物丙二醛(MDA),一氧化氮(NO)及抗氧化标志物超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GPx)水平,同时常规测定病例组空腹血糖、C-肽、糖化血红蛋白(HbA1_C)、尿素氮,并对结果进行差异性和相关性分析。结果 1型糖尿病患儿血清MDA,NO,SOD水平显著高于对照组(P<0.05),GPx水平显著低于对照组(P<0.01)。初发组MDA、NO、SOD水平显著高于对照组(P<0.05),GPx水平显著低于对照组(P<0.01)。病程稍长组SOD水平与对照组比较、感染组与非感染组间各指标比较,差异均无统计学意义(P>0.05)。MDA水平与HbA1_C水平呈显著正相关(r=0.377,P<0.01)。结论 1型糖尿病患儿发病初期及病程进展过程中存在明显的氧化应激水平升高和抗氧化保护能力下降,提示氧化应激可能在儿童1型糖尿病中起着重要作用。

关键词: 1型糖尿病, 氧化应激, 丙二醛, 一氧化氮, 超氧化物歧化酶, 谷胱甘肽过氧化物酶

Abstract: Objective To compare the serum levels of oxidative stress index in type 1 diabetic children and healthy children,and explore the changes of oxidative and anti-oxidative ability of children with type 1 diabetes mellitus. Methods A total of 62 type 1 diabetic children were selected as study cases,and 36 healthy children as normal controls.According to duration and infected situation,the study cases was divided into onset group and little long-term group,infected group and un-infected group.Serum levels of oxidative parameters:malondialdehyde (MDA),nitric oxide (NO),and anti-oxidative parameters:glutathione peroxidase (GPx),superoxide dismutase (SOD) were measured.At the same time,fasting blood glucose,c-peptide,glycosylated hemoglobin (HbA1c),blood urea nitrogen in study cases were detected.The significance and correlation among these parameters were analyzed. Results The serum MDA,NO,SOD levels in study cases were significantly higher than controls (P<0.05),while the levels of serum GPx level in cases was significantly lower than controls (P<0.05).In the onset group,MDA,NO,SOD were significantly higher than controls (P<0.05),GPx was significantly lower than controls,too (P<0.05).There was no significance between infected and un-infected groups(P>0.05).There was a positive correlation between levels of MDA and levels of HbA1c (r=0.376,P<0.01). Conclusion sThe significant increase of oxidative stress and decrease of anti-oxidative capacity occurr at onset and in the development of type 1 diabetes mellitus in children.Oxidative stress may take the important role in type 1 diabetes mellitus of children.

Key words: type 1 diabetes mellitus, oxidative stress, malondialdehyde, glutathione peroxidase, nitric oxide, superoxide dismutase

中图分类号:

R179

赵永鑫, 辛颖. 1型糖尿病儿童氧化应激水平的研究[J]. 中国儿童保健杂志, 2017, 25(7): 675-678.

ZHAO Yong-xin, XIN Ying. Status of oxidative stress in children with type 1 diabetes mellitus[J]. journal1, 2017, 25(7): 675-678.

参考文献

[1] Patterson CC,Dahlquist GG,gyurus E,et al.Incidence trends for childhood type 1 diabetes in Europe during 1989-2003 and predicted new cases 2005-20:a multicentre prospective registration study[J].Lancet,2009,373(9680):2027-2033.
[2] Gyurus EK,Patterson C,Soltesz G,et al.Twenty-one years of prospective incidence of childhood type 1 diabetes in Hungary-the rising trend continues (or peaks and highlands[J].Pediatr Diabetes,2012,13(1):21-25.
[3] Wang J,Yang X,Zhang J.Bridges between mitochondrial oxidative stress,ER stress and mTOR signaling in pancreatic βcells[J].Cell Signal,2016,28(8):1099-1104.
[4] Newsholme P,Haber EP,Hirabara SM,et al.Diabetes associated cell stress and dysfunction:role of mitochondrial and non-mitochondrial ROS production and activity[J].J Physiol,2007,583(Pt 1):9-24.
[5] Sakai K,Matsumoto K,Nishikawa T,et al.Mitochondrial reactive oxygen species reduce insulin secretion by pancreatic beta-cells[J].Biochem Biophys Res Commun,2003,300(1):216-222.
[6] Tian YF,Hsieh CH,Hsieh YJ,et al.α-Lipoic acid prevents mildportal endotoxaemia-induced hepatic inflammation and β cell dysfunction[J].Eur J Clin Invest,2012,42(6):637-648.
[7] Lau YS,Ling WC,Murugan D,et al.Boldine ameliorates vascular oxidative stress and endothelial dysfunction:Therapeutic implication for hypertension and diabetes[J].J Cardiovasc Pharmacl,2015,65(6):297-298.
[8] Lee BW,Kwon SJ,Chae HY,et al.Dose-related cytoprotective effect of alpha-lipoic acid on hydrogen peroxide-induced oxidative stress to pancreatic beta cells[J].Free Radic Res,2009,43(1):68-77.
[9] Runge MS,Molnar K,Madamanchi NR.“Old” hearts and arteries:the role of oxidative stress[J].Trans Am Clin Climatol Assoc,2010,121(121):52-58.
[10] Lin CC,Huang HH,Hu CW,et al.Trace elements,oxidative stress and glycemic control in young people with type 1 diabetes mellitus[J].J Trace Elem Med Biol,2014,28(1):18-22.
[11] Samahy MHE,Matter RM,Youssef OI,et al.Relation between carotid intima media thickness and oxidative stress markers in type 1 diabetic children and adolescents[J].J Diabetes Metab Disord,2013,12(1):50-56.
[12] Fatima N,Faisal SM,Zubair S,et al.Role of Pro-Inflammatory cytokines and biochemical markers in the pathogenesis of type 1 diabetes:correlation with age and glycemic condition in diabetic human subjects[J].PLoS One,2016,11(8):e0161548.doi:10.1371/journal.pone.0161548.
[13] Bandeira MDS,da Fonseca LJSD,Guedes GDS,et al.Oxidative stress as an underlying contributor in the development of chronic complications in diabetes mellitus[J].Int J Mol Sci,2013,14(2):3265-3284.
[14] Dominguez C,Ruiz E,Gussinye M,et al.Oxidative stress at onset and in early stages of type 1 diabetes in children and adolescents[J].Diabetes Care,1998,21:1736-1742.
[15] Martín-Gallán P,Carrascosa A,Gussinyé M,et al.Oxidative stress in childhood type 1 diabetes:Results from a study covering the first 20 years of evolution[J].Free Radic Res,2007,41(8):919-928.
[16] Indran M,Rokiah P,Chan SP,et al.Alteration of lipid peroxidation and antioxidant enzymes in young Malaysian IDDM patients[J].Med J Malaysia,2004,59(2):166-170.
[17] Firoozrai M,Nourbakhsh M,Razzaghy-Azar M.Erythrocyte susceptibility to oxidative stress and antioxidant status in patients with type 1 diabetes[J].Diabetes Research and Clinical Practice,2007,77:427-432.

1型糖尿病儿童氧化应激水平的研究

Status of oxidative stress in children with type 1 diabetes mellitus

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	沈富辉, 尹立琴, 王凡. 高同型半胱氨酸致神经管畸形的研究进展[J]. 中国儿童保健杂志, 2023, 31(2): 176-179.
[2]	陶凤姣, 温航卫, 刘作姣. 毛细支气管炎患儿血清高迁移率族蛋白B₁、可溶性清道夫受体163水平与呼出气一氧化氮浓度及病情严重程度的关系[J]. 中国儿童保健杂志, 2022, 30(3): 335-338.
[3]	黄馨琳, 赵琳. 维生素D影响新生儿黄疸的可能机制[J]. 中国儿童保健杂志, 2021, 29(9): 978-980.
[4]	刘阳, 高伟霞, 宋哲, 朱永杰. 呼出气一氧化氮联合白介素-17、白介素-27检测对儿童支气管哮喘的临床诊断价值[J]. 中国儿童保健杂志, 2021, 29(9): 1008-1011.
[5]	焦丽华, 厉红, 刘建丽. 血浆促肾上腺皮质激素、皮质醇水平与1型糖尿病患儿糖化血红蛋白水平的相关性研究[J]. 中国儿童保健杂志, 2021, 29(4): 377-380.
[6]	吴少辉, 黄晓冬, 徐智航. TLRs信号通路和炎症因子的表达与反复呼吸道感染的关系分析[J]. 中国儿童保健杂志, 2021, 29(2): 205-208.
[7]	常洁, 顾芳, 王杰英, 李荣敏, 雷淑琴, 李珍. 初发1型糖尿病患儿的血脂情况及与糖化血红蛋白水平的关系研究[J]. 中国儿童保健杂志, 2020, 28(8): 909-911.
[8]	刘永莉. 脐血中丙二醛、晚期氧化蛋白产物表达水平与胎儿窘迫及不良结局发生的相关性[J]. 中国儿童保健杂志, 2020, 28(7): 737-739.
[9]	李萌, 何学佳, 朱薇薇. 微生物-肠-脑轴在新生儿缺氧缺血性脑病发病机制中的研究进展[J]. 中国儿童保健杂志, 2020, 28(11): 1223-1226.
[10]	周焕珍, 杨柳, 王爱萍, 杨洋, 张红红. 1型糖尿病儿童肠道菌群谱分析研究[J]. 中国儿童保健杂志, 2020, 28(10): 1083-1087.
[11]	林丽娜, 李倩婷, 林娟, 江平湖, 黄宏. 1型糖尿病患儿父亲参与教养的现状及其与患儿生存质量的相关性分析[J]. 中国儿童保健杂志, 2020, 28(1): 104-107.
[12]	缪艳霞, 王芳, 乔良美, 刘怡欣, 常黎华. 学龄期1型糖尿病儿童生存质量差异的因素分析[J]. 中国儿童保健杂志, 2019, 27(5): 551-554.
[13]	常绍鸿,栾斌,张卫星. 内皮型一氧化氮合成酶基因多态性与早产儿脑病的相关性研究[J]. 中国儿童保健杂志, 2019, 27(2): 205-207.
[14]	韩玉敏, 石娜, 王燕, 刘立刚, 郅莉莉. 过敏性哮喘患儿一氧化氮、总免疫球蛋白E、调节性T细胞的表达及联合检测的意义[J]. 中国儿童保健杂志, 2019, 27(12): 1335-1338.
[15]	李文轩, 朱韵倩, 王晓明. 哮喘儿童来源的中性粒细胞经一氧化氮供体诱导形成胞外诱捕网的相关研究[J]. 中国儿童保健杂志, 2018, 26(8): 831-834.