锂对缺氧状态下新生鼠神经干细胞损伤的影响

中国儿童保健杂志 ›› 2013, Vol. 21 ›› Issue (10): 1045-1048.

锂对缺氧状态下新生鼠神经干细胞损伤的影响

王钰^1,2,尹晓娟^1,2

1 北京军区总医院附属八一儿童医院,北京 100700; 2 安徽医科大学北京军区总医院临床学院,安徽合肥 230001

收稿日期:2013-03-13 发布日期:2013-10-06 出版日期:2013-10-06
通讯作者: 尹晓娟,E-mail:yyinxiaojuan@163.com
作者简介:王钰(1986-),女,山东人,现为安徽医科大学北京军区总医院临床学院在读硕士研究生,主要从事新生儿脑修复的临床与基础研究。

Lithium-mediate influence on stem cells in vitro culture in neonate rat in anaerobic condition

WANG Yu^1,2,YIN Xiao-juan^1,2

1 Bayi Children's Hospital Affiliated to General Hospital of Beijing Military Command of People's Liberation Army,Beijing 100700,China; 2 Graduate School of Anhui Medical University,Institute of Beijing Military Region General Hospital,Hefei,Anhui 230001,China

Received:2013-03-13 Online:2013-10-06 Published:2013-10-06
Contact: YIN Xiao-juan,E-mail:yyinxiaojuan@163.com

摘要/Abstract

摘要： 目的培养神经干细胞,制造缺氧模型并探讨锂对缺氧损伤的神经干细胞是否具有保护作用。方法选取培养第四代的新生SD大鼠神经干细胞进行缺氧,细胞分为缺氧组和不缺氧组,每组内又分为不加锂组,加锂组(1、2、3、4、5mm)。免疫组织化学检测nestin、NSE和GFAP的表达。结果 95%N₂和5%CO₂混合气体培养细胞30min能成功构建离体神经干细胞缺氧损伤模型,一定浓度的锂能对缺氧损伤的神经干细胞发挥保护作用。结论锂对缺氧下神经干细胞有保护作用。

关键词: 神经干细胞, 缺氧, 锂

Abstract: Obiective To establish hypoxia damage model of neural stem cells (NSCs) and investigate the protection of lithium on them through culture NSCs. Methods NSCs were cultured from neonatal mice,immunocytochemistry was used to examine the Nestin expression of NSC,the forth passage cells were divided into control,hypoxia and lithium groups,NSE and GFAP were used to evaluate cell differentiation,cck-8 was used to evaluate cell proliferation,and NSE and GFAP were used to evaluate cell differentiation. Result NSCs under hypoxia 95%N₂,5%CO₂ for 30 minutes could establish hypoxia damage model in vitro and certain concentration of lithium protected the damages. Conclusion Lithium can protect NSCs under hypoxia and may prevent NSCs against hypoxia injury.

Key words: neural stem cells, hypoxia, lithium

中图分类号:

Q426

王钰,尹晓娟. 锂对缺氧状态下新生鼠神经干细胞损伤的影响[J]. 中国儿童保健杂志, 2013, 21(10): 1045-1048.

WANG Yu,YIN Xiao-juan. Lithium-mediate influence on stem cells in vitro culture in neonate rat in anaerobic condition[J]. journal1, 2013, 21(10): 1045-1048.

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