Abstract: Objective To investigate the mechanism of calcium binding protein A6 (S100A6) mediated by terminal glycosylation product receptor (RAGE) on vascular endothelial injury in obese children,in order to provide the basis for further targeted treatment programs. Methods Totally 91 obese children admitted to Shiyan Maternal and Child Health Hospital from July 2015 to July 2018 were divided into injury group (n=43) and non-injury group (n=48) according to the presence or absence of vascular endothelial injury.Meanwhile,45 healthy children who underwent physical examination in this hospital during the same period were selected as control group.Serum levels of S100A6 and free RAGE (sRAGE) were measured on the day of admission in all three groups.The levels of vascular endothelial injury markers including vascular endothelial adhesion molecule-1 (sVCAM-1),soluble cell adhesion molecule-1 (sICAN-1) and von Willebrand factor (vWF) were measured by ELISA. Results The levels of serum S100A6 and sRAGE in the injured group were significantly higher than those in the non-injured group and the control group (P<0.05).The serum levels of sVCAM-1,vWF and sICAN-1 in the injured group were higher than those in the non-injured group and the control group (P < 0.05).Pearson linear correlation analysis showed that S100A6 and sRAGE levels were positively correlated with sVCAM-1,vWF and sICAN-1 levels(P<0.05). Conclusions Compared with normal children and obese children without vascular endothelial injury,the serum levels of S100A6 and sRAGE are significantly increased in obese children with vascular endothelial injury,which may be related to the increase of sRAGE leading to the increase of S100A6,thereby further increasing sVCAM-1,vWF and sICAN-1 levels.

Key words: obese children, vascular endothelial injury, calcium binding protein A6, terminal glycosylation product receptor

摘要： 目的 探讨钙结合蛋白A6(S100A6)经终末糖基化产物受体(RAGE)介导影响肥胖儿童血管内皮损伤的机制,为进一步提出有针对性的治疗方案提供依据。方法 选取十堰市妇幼保健院2015年7月-2018年7月收治的肥胖患儿91例,根据患儿是否存在血管内皮损伤分成损伤组(n=43)、无损伤组(n=48)。选取同期于本院体检的健康体检儿童45例作为对照组。三组受检者均于入院当日采血检测血清S100A6与游离RAGE(sRAGE)水平,并测定三组血管内皮损伤标志物,包括血管内皮黏附分子-1(sVCAM-1)、可溶性细胞黏附分子-1(sICAN-1)、血管性血友病因子(vWF)水平,检测方法为酶联免疫吸附法。经Pearson线性相关分析S100A6、sRAGE与血管内皮损伤标志物间的相关性。结果 损伤组血清S100A6、sRAGE水平高于无损伤组、对照组,差异有统计学意义(P<0.05)。损伤组血清sVCAM-1、vWF、sICAN-1水平高于无损伤组、对照组,差异有统计学意义(P<0.05)。经Pearson线性相关分析提示S100A6、sRAGE与sVCAM-1、vWF、sICAN-1均呈正相关(P<0.05)。结论 与正常儿童及无血管内皮损伤的肥胖儿童相比,有血管内皮损伤的肥胖儿童血清S100A6、sRAGE明显上调,这可能与sRAGE增高导致S100A6上调,而进一步致sVCAM-1、vWF、sICAN-1水平增高有关。

关键词: 肥胖儿童, 血管内皮损伤, 钙结合蛋白A6, 终末糖基化产物受体