Abstract: Objective To study the changes in serum levels of neurokinin B (NKB) and neuropeptide (Kisspeptin) in girls with idiopathic central precocious puberty (ICPP), and to analyze the correlation with the bone age index, so as to provide reference for assessing the development of these children and the efficacy of treatment. Methods A total of 89 cases of ICPP girls treated in Luoyang Maternal and Child Health Hospital from June 2017 to May 2019 were enrolled in this study, and meanwhile 90 healthy girls were selected as the control group.The levels of serum NKB, Kisspeptin, hormone, bone age and bone age index (BAI) of the participants were detected.The changes of the indicators were compared before and after treatment.Pearson analysis was used to analyze the correlation among serum NKB, Kisspeptin levels and BAI in girls with ICPP before treatment.Logistic regression analysis was used to analyze the influencing factors of bone age index (bone development). Results Compared with those in the control group, the bone age, BAI, uterine volume, serum estrogen, luteinizing hormone, follicle stimulating hormone, prolactin, NKB and Kisspeptin levels were significantly increased in ICPP group (t=14.245, 22.089, 8.321, 18.619, 27.727, 14.241, 28.152,17.655,9.668, P<0.05).Pearson correlation analysis showed that serum NKB and Kisspeptin levels were positively correlated with BAI (r=0.591, 0.634, P<0.05).Logistic regression analysis showed that NKB(OR=2.009, 95%CI:1.105 - 3.654, P=0.038) and Kisspeptin(OR=1.859, 95%CI:1.341 - 2.578, P=0.017) are factors that affect bone age index (bone development).Compared with those before treatment, the levels of serum estrogen, luteinizing hormone, follicle stimulating hormone, prolactin, NKB, Kisspeptin and BAI in ICPP girls were significantly decreased after treatment (t=14.367, 22.482, 9.465, 23.807, 13.664, 8.642, 7.100, P<0.05). Conclusions The serum levels of NKB and Kisspeptin in ICPP girls are increasing, which is related to the patient's bone age index and can be used as markers to assess the sexual development of ICPP girls and the effect of treatment.

Key words: neurokinin B, kisspeptin, idiopathic central precocious puberty, bone age index, girls, correlation, serum

摘要： 目的 研究特发中枢性性早熟(ICPP)女童血清神经激肽B(NKB)、神经肽吻素(kisspeptin)水平变化及其与骨龄指数(BAI)的相关性,以期寻找评估此类儿童发育情况及治疗效果的标志物。方法 选择2017年6月-2019年5月在洛阳市妇幼保健院治疗的ICPP女童89例,另选取同期健康体检女童90例作为对照组,检测血清NKB、Kisspeptin、激素水平及骨龄、骨龄指数(BAI)等,比较治疗前、治疗后患者各指标变化,采用Pearson分析ICPP女童治疗前血清NKB、Kisspeptin水平与BAI相关性,Logistic回归分析影响骨龄指数(骨发育)的因素。结果 与对照组比较,ICPP组女童骨龄、BAI、子宫体积、血清雌激素、黄体生成素、卵泡刺激素、催乳素及NKB、Kisspeptin水平均显著升高,差异均有统计学意义(t=14.245、22.089、8.321、18.619、27.727、14.241、28.152,17.655,9.668,P<0.05);相关性分析显示,治疗前ICPP女童血清NKB、Kisspeptin水平与BAI呈正相关(r=0.591、0.634,P<0.05);Logistic回归分析显示NKB(OR=2.009,95%CI:1.105~3.654,P=0.038)、Kisspeptin(OR=1.859,95%CI:1.341~2.578,P=0.017)是骨龄指数(骨发育)的影响因素;治疗后ICPP女童血清雌激素、黄体生成素、卵泡刺激素、催乳素、NKB、Kisspeptin水平及BAI均显著降低,与治疗前比较差异均有统计学意义(t=14.367、22.482、9.465、23.807、13.664、8.642、7.100,P<0.05)。结论 ICPP女童血清NKB、Kisspeptin水平升高,与患者骨龄指数有关,可作为评估ICPP女童性发育情况及治疗效果的标志物。

关键词: 神经激肽B, 神经肽吻素, 特发中枢性性早熟, 骨龄指数, 女童, 相关性, 血清