Abstract: Objective To investigate the effects of high zinc intake in early life on insulin levels in adulthood with high fat diet. Methods Totally 85 male SD weanling rats were randomly divided into basic diet group,high zinc group,medium zinc group and low zinc group.After 4 weeks,all rats were fed with basic diet for 1 week,then Fasting plasma glucose and fasting plasma insulin were detected,and the insulin sensitively index were calculated.The basic diet group then were randomly divided into obesity induction group and normal control group.Three zinc supplementation groups and obesity induction group were fed with high fat diet for 8 weeks.All rats were then put to death,the above indicators were detected and calculated. Results After high fat intervention,1)the body weight of three zinc supplementation groups were significantly lower than that of obesity induction group (P＜0.05);2) fasting plasma glucose and fasting plasma insulin of three zinc supplementation groups were significantly lower than those of obesity induction group (P＜0.05),and the insulin sensitively index was significantly higher (P＜0.05). Conclusion Appropriate zinc supplement in early life can play a continuous function,it can maintain the fasting plasma glucose and fasting insulin at normal levels to a certain extent after adulthood with high fat diet and prevent insulin resistance.

Key words: weanling rat, zinc supplementation, high-fat intervention, insulin resistance

摘要： 目的 探讨初断乳大鼠锌补充对成年期高脂饮食下胰岛素水平的影响,为阐明生命早期适量补锌预防成年后胰岛素抵抗的机制提供重要依据。方法 初断乳雄性SD大鼠85只随机分为基础饲料组和高、中、低锌补充组。锌补充4周后各组均以基础饲料喂养1周,于第5周末检测大鼠血糖、胰岛素水平并计算胰岛素抵抗指数。随后将基础饲料组大鼠随机分为肥胖组和正常对照组,肥胖诱导组及3个锌补充组均喂以高脂饲料。高脂干预8周后处死所有大鼠并检测上述指标。结果 大鼠成年高脂干预后,1)3个锌补充组分别较肥胖诱导组体重明显降低(P＜0.05),高脂饲料喂养后锌补充组体重正常,而肥胖诱导组发生肥胖;2)3个锌补充组分别较肥胖诱导组血糖、胰岛素明显降低(P＜0.05),而胰岛素抵抗指数明显升高(P＜0.05),高脂饲料喂养后锌补充组胰岛素水平正常,而肥胖诱导组产生胰岛素抵抗。结论 生命早期适量补锌可持续发挥作用,在一定程度上维持成年后高脂膳食下血糖和胰岛素处于正常水平,预防胰岛素抵抗发生。

关键词: 初断乳大鼠, 锌补充, 高脂干预, 胰岛素抵抗