Abstract: Objective To study the expression and significance of serum miRNA-21 and hypoxia inducible factor-1α (HIF-α) in newborns with asphyxia. Methods A total of 78 newborn infants were recruited in the study,including 49 newborn infants with asphyxia and 29 cases without asphyxia.In 24~48 h after birth,the serum miRNA-21 and hypoxia inducible factor-1α (HIF-1α) were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Result The levels of serum miRNA-21 and HIF-1α in newborn infants with asphyxia were high than those of control group (P<0.05). Conclusions Serum miRNA-21 plays a role on the hypoxic brain damage after asphyxia by acting on the target genes of HIF-1α.The serum expression of miRNA-21 can be used as a marker in the early diagnosis of hypoxic brain damage after asphyxia.

Key words: asphyxia, newborn infant, miRNA-21, hypoxia inducible factor-1α, quantitative real-time polymerase chain reaction

摘要： 目的 探讨新生儿缺氧缺血性脑病血清中 miRNA- 21、缺氧诱导因 子1(HIF-1α)的表达及临床意义,为临床上早期诊断及治疗缺氧缺血性脑病提供科学依据。方法 选取2012年1月-2013年6月在新乡市中心医院和妇幼保健院出生的新生儿,分为:缺血缺氧组49例;正常对照组29例,共计78 例。两组于生后 24~48 h 内采血5 mL。应用实时荧光定量 PCR 技术检测血清中 miRNA-21和HIF-1α的表达水平。结果 缺氧缺血组与对照组相比,缺氧缺血组血清中miRNA-21和HIF-1α均呈现高表达(P＜0.05)。结论 血清中miRNA-21和HIF-1α的高表达与缺氧缺血性脑病有关,并可作为早期判断脑损伤的有用的生物学指标。

关键词: 窒息, 新生儿, 微小RNA-21, 缺氧诱导因子-1α, 实时荧光定量PCR