Abstract: Objective To observe the change of the synapse associated protein synaptophysin (SYN),postsynappptic density protein-95 (PSD-95),gephyrin in the prefrontal cortex of the rat model of autism and explore the role of synapse associated protein in the pathogenesis of autism. Method sThe autism model group was obtained in the offspring female Wister rats those received a single intraperitoneal injection of sodium valproate (600mg/kg) on the 12.5th pregnancy day.Pregnant rats received the intraperitoneal injection of saline,whose offspring were defined as normal group.To confirm whether the animal model were successfully established,the model group were compared the oblique board test,opening eyes test and three-chamber sociability test with control group.Western blotting was used to detected the expressed level of SYN,PSD-95,gephyrin in prefrontal cortex of model group and control group in the 42th day after birth. Results 1)The animal model of autism was successfully established.Compared with control rats,the autistic ones were slower to grow,poorer social interaction ability,and the preference for fresh things was disorder,the differences were statistically significant(all P<0.05);2)Compared with control group,the model group significantly increased the expression level of SYN and PSD-95 (P<0.05) and decreased the expression level of gephyrin(P<0.05) in prefrontal cortex. Conclusion The expression of synaptic associated proteins in the prefrontal cortex of autism rats is abnormal.

Key words: autism, synaptophysin, postsynappptic density protein-95, gephyrin, prefrontal cortex

摘要： 目的 观察孤独症大鼠突触相关蛋白突触素(SYN)、突触后致密蛋白-95(PSD-95)、桥尾蛋白(gephyrin)在前额叶皮质的表达变化,探讨突触相关蛋白在孤独症发病中的作用。方法 采用孕12.5 d(E12.5)一次性腹腔注射丙戊酸钠(600 mg/kg)的孕鼠所产下的子代鼠作为模型组,同样方法注射同等剂量的生理盐水的孕鼠所产下子代鼠作为正常组。通过斜板实验、睁眼实验、三箱实验验证模型是否成功;Western blotting方法对比生后42天两组大鼠突触相关蛋白SYN、PSD-95、gephyrin在前额叶皮质的表达变化。结果 1)成功建立孤独症动物模型:与正常组比较,模型组大鼠生长发育迟缓;社会交往能力异常、对新鲜事物偏好障碍,差异均有统计学意义(P均<0.05);2)Western blotting结果显示:与正常组比较,孤独症大鼠突触相关蛋白SYN、PSD-95表达显著增多(P<0.05),gephyrin蛋白表达显著减少(P<0.05)。结论 孤独症大鼠前额叶皮质突触蛋白表达异常。

关键词: 孤独症, 突触素, 突触后致密蛋白-95, 桥尾蛋白, 前额叶皮质