Effect of ω-3 PUFAs on regulation of Toll-like receptor 4 and nuclear factor-ΚB signaling pathway in neonatal rats with brain injury induced by LPS

doi:10.11852/zgetbjzz2018-26-02-12

journal1 ›› 2018, Vol. 26 ›› Issue (2): 158-162.DOI: 10.11852/zgetbjzz2018-26-02-12

Effect of ω-3 PUFAs on regulation of Toll-like receptor 4 and nuclear factor-ΚB signaling pathway in neonatal rats with brain injury induced by LPS

SHI Ji-peng¹, LI Yan-wei², SONG Ya-hui³, ZHANG Li-li, SANG Gui-mei¹, GUO Hong-xun¹, WANG Wei-wei¹, TANG Cheng-he¹, Huang Li-mi⁴

1 Department of Neonatology,the First Affiliated Hospital of Xinxiang Medical University,Xinxiang,Henan 453100,China
2 Department of Neurology,The Third Affiliated Hospital of Xinxiang Medical University,Xinxiang,Henan 453000
3 Department of Echo Lab,Nanyang Second People's Hospital,Nayang,Henan 473000,China
4 Department of Gastroenterology,the First Affiliated Hospital of Xinxiang Medical University,Xinxiang,Henan 453100,China
5 Department of Pediatrics,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou,Zhejiang 325000,China

Received:2017-12-28 Online:2018-02-10 Published:2018-02-10
Contact: HUANG Li-mi,E-mail:hlm0201262@126.com

ω-3多不饱和脂肪酸对脂多糖所致新生大鼠脑损伤Toll-NF-κB信号通路调节作用

石计朋¹, 栗延伟², 宋亚辉³, 张利利⁴, 桑桂梅¹, 郭洪旭¹, 王卫卫¹, 唐成和¹, 黄丽密⁵

1 新乡医学院第一附属医院新生儿科,河南新乡 453100
2 新乡医学院第三附属医院神经内科,河南新乡 453000
3 南阳市第二人民医院超声科,河南南阳 473000
4 新乡医学院第一附属医院消化内科,河南新乡 4531 00
5 温州医科大学附属第一医院儿科,浙江温州 325000

通讯作者: 黄丽密,E-mail:hlm0201262@126.com
作者简介:石计朋(1981-),男,河南人,主治医师,硕士学位,博士研究生在读,主要研究方向为新生儿疾病。
基金资助:
河南省医学科技攻关计划项目(201602148);河南省神经修复重点实验室开放课题(HNSJXF-2016-017)

Abstract

Abstract: Objective To investigate the effects of ω-3 PUFAs on regulation of Toll-like receptor 4 (TLR4) and nuclear factor-κB (NF-κB) signaling pathway in neonatal rats with brain injury induced by LPS. Methods Totally 96 neonatal Sprague-Dawley rats were divided into control group,ω-3 group,ω-6 group,and LPS group.Intraperitoneal injection with saline or LPS was administered and immediately saline or fat emulsions was injected to establish a model of rat with brain injury induced by infection and the duration time was 1 day or 5 days respectively.Those groups were sacrificed at 1d or 5 d after intraperitoneal injection with saline or fat emulsions.Expression levels of TLR4 mRNA and NF-κB mRNA were examined by real-time PCR,meanwhile the levels of TLR4,and NF-κB in the hippocampus were measured by Western Blot. Results The expressions of TLR mRNA and NF-κB mRNA and the protein level of TLR4 and NF-κB in the hippocampus were significantly different in four groups(all Pˉ0.05).TLR mRNA and NF-κB mRNA and the protein expression levels in ω-3 group were lower than those in ω-6 group and LPS group on 1 d after injection (all Pˉ0.05).And the protein expression levels of TLR mRNA and NF-κB mRNA in ω-3 group were lower than those in ω-6 group and LPS group on five days after injection (all Pˉ0.05).No matter the first day or the fifth day,the expression of TLR mRNA and NF-κB mRNA and the level of TLR4 and NF-κB in ω-3 group were significantly lower than those in ω-6 group(all Pˉ0.05). Conclusion ω-3 PUFAs can down regulate the activity of signaling pathway of TLR4 and NF-κB,so it may has an anti-inflammatory effect on brain injuryinduced by LPS.

Key words: neonatal rats, ω-3 PUFAs, ω-6 PUFAs, brain injury, LPS, TLR4, NF-κB

摘要： 目的探讨ω-3 PUFAs在脂多糖(LPS)诱导的新生大鼠脑损伤中对TLR4、NF-κB信号通路的调节作用。方法将96只SD新生大鼠按随机数字法分为对照组,ω-3组,ω-6组和LPS组。4组大鼠分别采用生理盐水或LPS腹腔注射后,立即注射生理盐水或不同的脂肪乳剂,使用时间分别为1天或5天,建立感染所致新生大鼠脑损伤模型;1天或5天后处死新生大鼠,检测TLR4 mRNA、NF-κB mRNA和对应蛋白水平的变化。结果 LPS注射后的第1天、第5天,新生大鼠TLR4 mRNA、NF-κB mRNA表达及其对应的蛋白水平均明显高于对照组(P均＜0.05)。1 d 时ω-3组TLR4 mRNA、NF-κB mRNA表达及其对应的蛋白水平均低于ω-6组和LPS组(P均＜0.05)。5 d时ω-3组TLR4 mRNA、NF-κB mRNA表达及其对应的蛋白水平均低于ω-6组和LPS组(P均＜0.05)。不论第1 d和第5 d,ω-3 PUFAs均可明显降低TLR4 mRNA、NF-κB mRNA表达及其对应的蛋白水平(P均＜0.05);而ω-6 PUFAs能提高TLR4 mRNA、NF-κB mRNA表达及对应的蛋白水平(P均＜0.05)。结论 ω-3 PUFAs 能下调 TLR4、NF-κB活性,在LPS所致的脑损伤中具有抗炎作用。

关键词: 新生大鼠, ω-3PUFAs, ω-6PUFAs, 脑损伤, 脂多糖, TLR4、NF-κB

CLC Number:

R179

SHI Ji-peng, LI Yan-wei, SONG Ya-hui, ZHANG Li-li, SANG Gui-mei,
GUO Hong-xun, WANG Wei-wei, TANG Cheng-he, Huang Li-mi. Effect of ω-3 PUFAs on regulation of Toll-like receptor 4 and nuclear factor-ΚB signaling pathway in neonatal rats with brain injury induced by LPS[J]. journal1, 2018, 26(2): 158-162.

石计朋, 栗延伟, 宋亚辉, 张利利, 桑桂梅, 郭洪旭, 王卫卫, 唐成和, 黄丽密. ω-3多不饱和脂肪酸对脂多糖所致新生大鼠脑损伤Toll-NF-κB信号通路调节作用[J]. 中国儿童保健杂志, 2018, 26(2): 158-162.

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Effect of ω-3 PUFAs on regulation of Toll-like receptor 4 and nuclear factor-ΚB signaling pathway in neonatal rats with brain injury induced by LPS

ω-3多不饱和脂肪酸对脂多糖所致新生大鼠脑损伤Toll-NF-κB信号通路调节作用

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