Abstract: Objective To investigate relationship between IL-6 and TNF-α in umbilical cord blood of neonates with preterm premature rupture of membranes (PPROM) and chioroamnionitis. Methods Totally 45 cases of pregnant women with premature rupture of membranes in obstetric department of Affiliated Hospital of Yanbian University were enrolled as observation group from September 2016 to January 2017,who were with singleton,head position,gestational age of 28~37 w and production process launched within 12 hours after rupture of membranes.While 45 cases of pregnant women with full pregnancy were enrolled as control group in the same period,and the production times of pregnant women were 0~1.And 5 ml umbilical cord blood were taken after cutting the neonatal umbilical cord.The levels of IL-6 and TNF-α in cord blood of newborn infants were measured by flow cytometry.At the same time,placenta and fetal membrane tissues of the pregnant women with PPROM were send to pathological examination,in order to diagnose chorionic inflammation. Results There were no significantly statistical differences on the levels of IL-6 and TNF-α in umbilical cord blood of newborn infants between observation group and control group(P>0.05);There were significantly statistical differences in the levels of IL-6 and TNF-α in umbilical cord blood between pregnant women with or without chioroamnionitis(P<0.05). Conclusion Increasing levels of IL-6 and TNF-α in umbilical cord blood of neonates with PPROM are associated with choriocarcinoma.

Key words: premature delivery, preterm premature rupture of membranes, tumor necrosis factor-α, interleukin-6, chorioamnionitis

摘要： 目的 分析未足月胎膜早破新生儿脐血中IL-6、TNF-α含量与绒毛膜炎的相关性。方法 选择2016年9月-2017年1月于延边大学附属医院产科住院分娩的未足月(28周≤孕周＜37周)、单胎、头位合并胎膜早破的孕妇45例做为观察组,且均在破膜后12个小时内发动产程;同一时期内选择足月妊娠分娩的孕妇45例做为对照组。两组孕妇产次均为0~1次。新生儿断脐后取脐静脉血5 ml,采用流式细胞术测定新生儿脐血中IL-6、TNF-α的含量,同时将观察组孕妇的胎盘、胎膜进行病理学检查,确定有无绒毛膜炎。结果 观察组脐血中IL-6、TNF-α含量与对照组比较,差异无统计学意义(P＞0.05);绒毛膜炎组脐血中IL-6、TNF-α含量与非绒毛膜炎组比较,差异有统计学意义(P＜0.001)。结论 未足月胎膜早破新生儿脐血中IL-6、TNF-α水平升高与发生绒毛膜炎相关。

关键词: 早产, 未足月胎膜早破, 白介素-6, 肿瘤坏死因-α, 绒毛膜炎