Research on the association of interleukin-6 and interleukin-1β gene polymorphism with severity of hand foot and mouth disease induced by enterovirus 71

doi:10.11852/zgetbjzz2018-26-11-18

journal1 ›› 2018, Vol. 26 ›› Issue (11): 1228-1232.DOI: 10.11852/zgetbjzz2018-26-11-18

Previous Articles Next Articles

Research on the association of interleukin-6 and interleukin-1β gene polymorphism with severity of hand foot and mouth disease induced by enterovirus 71

LI Mei¹, LI Ya-ping¹, DENG Hui-ling^1,3, ZHANG Wen², MA Le¹, WANG Li-qun¹, WU Yuan¹

1 Department of Infectious Diseases, the Second Affiliated Hospital of Medical College,Xi′an Jiaotong University, Xi′an,Shaanxi 710004,China;
2 Department of Clinical Laboratory, the Second Affiliated Hospital of Medical College, Xi′an Jiaotong University, Xi′an, Shaanxi 710004,China;
3 Department of Infectious Diseases, Xi′an Children′s Hospital, Xi′an 710043, China

Received:2018-05-07 Revised:2018-06-11
Contact: LI Ya-ping, E-mail:liyaping8605@126.com

EV71感染手足口病严重程度与IL-6、IL-1β基因多态性的相关性研究

李梅¹,李亚萍¹,邓慧玲^1,3,张雯²,马乐¹,王立群¹,吴媛¹

1 西安交通大学第二附属医院感染科,陕西西安 710004;
2 西安交通大学第二附属医院检验科,陕西西安 710004;
3 西安市儿童医院感染科,陕西西安 710043

通讯作者: 李亚萍,E-mail:liyaping8605@126.com
作者简介:李梅(1986-),女,陕西人,主治医师,硕士学位,主要研究方向为感染性疾病。
基金资助:
国家自然科学基金(81701632);陕西省社会发展攻关项目(2017SF-154)

Abstract

Abstract: Objective To investigate the association of inflammatory cytokines including interleukin-6 (IL-6), IL-6R, IL-1β and IL-1RN gene polymorphism with severity of hand foot and mouth disease (HFMD) induced by enterovirus 71 (EV71), in order to provide evidence for early recognition and assessment of severe HFMD.Methods A total of 180 EV71 HFMD cases including 108 severe cases and 72 mild cases from the Second Affiliated Hospital of Xi′an Jiaotong University and Xi′an Children′s Hospital were randomly enrolled in this study. All participants were genotyped for the most common IL-6, IL-6R, IL-1β and IL-1RN single-nucleotide polymorphisms (SNPs) using the SNPscan multiple SNP typing method. The gene type of rs1800796, rs2069837 of IL-6, rs2228145, rs4845617 of IL-6R,rs16944, rs1143627 of IL-1β and rs315952, rs9005 of IL-1R among 180 cases were detected by SNPscan kit.Results The severity risk was significantly increased in IL-6 rs1800796 allele G(OR=1.312, 95% CI:1.041~1.653)and IL6-R rs2228145 allele C (OR=1.330, 95% CI:1.064～1.663), while it was reduced in IL-1β rs1143627 allele G (OR=0.790,95% CI:0.635~0.981). No association of gene polymorphism with severity of EV71 HFMD was observed in IL-6 rs2069837, IL-6R rs4845617, IL-1β rs16944, IL-1RN rs315952 and IL-1RN rs9005.Conclusion The gene polymorphism of IL-6 rs1800796, IL-6R rs2228145 and IL-1β rs1143627 were associated with severity of EV71 HFMD.

Key words: enterovirus 71, interleukin-6, interleukin-1β, polymorphism, severity

摘要： 目的探讨炎性细胞因子白介素-6(IL-6)、IL-6R、IL-1β、IL-1RN基因多态性与肠道病毒71型(EV71)感染手足口病(HFMD)的严重程度的相关性,为重症HFMD的早期发现和患病风险的评估提供依据。方法以西安市儿童医院和西安交通大学第二附属医院感染科收住的180例EV71 HFMD患儿为研究对象,其中重症组108例,普通组72例。采用SNPscan^TM多重SNP技术检测上述样本IL-6 rs1800796、rs2069837,IL-6R rs2228145、rs4845617,IL-1β rs16944、rs1143627,IL-1RN rs315952、rs9005位点的基因分型。结果 180例EV71感染患儿中,IL-6 rs1800796 G等位基因的重症化风险升高(OR=1.312,95%CI:1.041~1.653),IL6-R rs2228145 C等位基因重症化风险升高(OR=1.330,95%CI:1.064~1.663),而IL-1β rs1143627 G等位基因是保护因素(OR=0.790,95%CI:0.635~0.981)。IL-6 rs2069837、IL-6R rs4845617,IL-1β rs16944、IL-1RN rs315952、rs9005的基因多态性与疾病严重程度无明显相关性。结论 IL-6 rs1800796、IL-6R rs2228145、IL-1β rs1143627基因多态性与EV71 HFMD患儿严重程度相关联。

关键词: 肠道病毒71型, 白介素-6, 白介素-1β, 基因多态性, 重症化

CLC Number:

R179

LI Mei, LI Ya-ping, DENG Hui-ling, ZHANG Wen, MA Le, WANG Li-qun, WU Yuan. Research on the association of interleukin-6 and interleukin-1β gene polymorphism with severity of hand foot and mouth disease induced by enterovirus 71[J]. journal1, 2018, 26(11): 1228-1232.

李梅,李亚萍,邓慧玲,张雯,马乐,王立群,吴媛. EV71感染手足口病严重程度与IL-6、IL-1β基因多态性的相关性研究[J]. 中国儿童保健杂志, 2018, 26(11): 1228-1232.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu41.magtech.com.cn/Jwk_zgetbjzz/EN/10.11852/zgetbjzz2018-26-11-18

https://manu41.magtech.com.cn/Jwk_zgetbjzz/EN/Y2018/V26/I11/1228

References

[1] Kushner D, Caldwell BD. Hand-foot-and-mouth disease[J]. J Am Podiatr Med Assoc,1996,86(6):257-259.
[2] Ooi MH, Solomon T, Podin Y, et al. Evaluation of different clinical sample typesin diagnosis of human enterovirus71 associated hand foot and mouth disease[J]. J Clin Microbiol,2007,45(6):1858-1866.
[3] Huang CC, Liu CC, Chang YC, et al. Neurologic complications in children with enterovirus 71 infection[J]. N Engl J Med,1999,341(13):936-942.
[4] Ye N,Gong X,Pang LL, et al. Cytokine responses and correlations thereof with clinical profiles in children with enterovirus 71 infections[J]. BMC Infect Dis,2015,15:225.
[5] Wang SM,Lei HY,Liu CC. Cytokine immunopathogenesis of enterovirus 71 brain stem encephalitis[J]. Clin Dev Immunol,2012,2012:876241.
[6] 中华人民共和国国家卫生和计划生育委员会.《手足口病诊疗指南(2010年版)》[DB/OL].[2010-04-20].http://www.moh.gov.cn/mohyzs/s3586/201004/46884.shtml
[7] Bi D, Chen M, Zhang X, et al. The association between sex-related interleukin-6 gene polymorphisms and the risk for cerebral palsy[J]. J Neuroinflammation,2014,11:100.
[8] Pathinayake PS, Hsu AC, Wark PA.Innate immunityand immune evasion by enterovirus 71[J]. Viruses,2015,7(12):6613-6630.
[9] 李亚萍,翟嵩,李梅等. TLR7基因rs3853839、rs179010多态性与EV71手足口病男性患儿易感性及病情严重程度相关[J].细胞与分子免疫学杂志,2017,33(7):953-958.
[10] 李亚萍,党双锁. 手足口病基因多态性的研究进展[J]. 临床医学研究与实践,2017,8:192-195.
[11] Patel JA, Nair S, Ochoa EE, et al. Interleukin-6-174 and tumor necrosis factor α-308 polymorphisms enhance cytokine production by human macrophages exposed to respiratory viruses[J]. J Interferon Cytokine Res,2010,30(12):917-921.
[12] Lin TY,Chang LY,Huang YC,et al. Different proinflammatory reactions in fatal and non-fatal enterovirus 71 infections:implications for early recognition and therapy[J]. Acta Paediatr, 2002, 91(6):632-635.
[13] Khong WX,Foo DG,Trasti SL,et al. Sustained high levels of interleukin-6 contribute to the pathogenesis of enterovirus 71 in a neonate mouse model[J]. J Virol,2011,85(7):3067-3076.
[14] 吕铁钢,王晓梅,李秋波,陈宗波. 肠道病毒71型感染乳鼠脑组织白介素1β与白介素6水平的研究[J].中国儿童保健杂志,2017,25(12):1222-1224.
[15] Yuan A,Li J,Liu P,et a1.Association of intedeukin-6-572c/G gene polvmorphism and serum or cerebrospinal fluid interleukin-6 level with enterovims 71 encephalitis in Chinese Han patients with hand foot and mouth disease[J].Innammation,2015,38(2):728-735
[16] 甘拓域,王成,龚晓辉,等.白细胞介素6基因RSl800796位点多态性与肠道病毒71型感染的相关性[J].中华实用儿科临床杂志,2017,32(6):457-460.
[17] Zhang JZ, Liu CM, Peng HP et al. Association of genetic variations in IL-6/IL-6R pathway genes with gastric cancer risk in a Chinese population[J]. Gene,2017,623:1-4.
[18] Ahmed S,Hussain S,Ammar A,et al. Interleukin 6 receptor (IL6-R) gene polymorphisms underlie susceptibility to rheumatoid arthritis[J]. Clin Lab,2017,63(9):1365-1369.
[19] Dinarello CA.IL-1:discoveries, controversies and future directions[J]. Eur J Immunol,2010,40(3):599-606.
[20] Wang Y,Qin Y,Wang T,et al. Pyroptosis induced by enterovirus 71 and coxsackie virus B3 infection affects viral replication and host response[J]. Sci Rep,2018,8(1):2887.

Research on the association of interleukin-6 and interleukin-1β gene polymorphism with severity of hand foot and mouth disease induced by enterovirus 71

EV71感染手足口病严重程度与IL-6、IL-1β基因多态性的相关性研究

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

[1]	ZHANG Chao. Application of early start Denver model in the rehabilitation of children with autism spectrum disorder of different conditions [J]. Chinese Journal of Child Health Care, 2023, 31(7): 736-740.
[2]	LI Jia, ZHANG Yanchi. Research advances on the correlation of gene polymorphisms of vitamin D receptor and vitamin D level with neurodevelopmental disorders in children [J]. Chinese Journal of Child Health Care, 2023, 31(5): 516-520.
[3]	LI Zhenghua, LIANG Cailing, HE Yi, LIU Qi, WANG Ji, ZHANG Lijun. Sleep problems of children with autism spectrum disorder and its influencing factors [J]. Chinese Journal of Child Health Care, 2023, 31(3): 316-321.
[4]	XIAO Wu-cai, LIU Zheng, CHEN Jing, WANG Hui, SONG Jie-yun, ZHOU Shuang, GAO Ai-yu, ZHANG Fang, WANG Hai-jun. Relationship between rs2241423 polymorphism of MAP2K5 gene and intervention effect of childhood obesity [J]. Chinese Journal of Child Health Care, 2022, 30(9): 952-955.
[5]	YOU Hai-zhen, ZHOU Yi-fang, XIE Jing, JIN Zhi-juan, WANG Guang-hai, SUN Ke-xing. Correlation between serum vitamin D and tic disorder [J]. Chinese Journal of Child Health Care, 2022, 30(8): 904-907.
[6]	SHANG Li, XU Pei-ru, AIERKEN Aibibai, WANG Yi-lin. Association of the single nucleotide polymorphism of NOS3 gene with obesity in children aged 6~13 years [J]. Chinese Journal of Child Health Care, 2022, 30(5): 490-494.
[7]	TAO Feng-jiao, WEN Hang-wei, LIU Zuo-jiao. Relationship between serum high mobility group box-1 protein, soluble cluster differentiation 163 levels andfractional exhaled nitric oxide concentration and severity of bronchiolitis in children [J]. Chinese Journal of Child Health Care, 2022, 30(3): 335-338.
[8]	YANG Li-juan, DENG Gao, DING Bo, YANG Li-min, LI Dong-ming, HUANG Yong-fang, LIN Na. Association of ADAM33 gene and its serum level with the risk of asthma in Zhuang children in Guangxi [J]. Chinese Journal of Child Health Care, 2022, 30(11): 1185-1190.
[9]	ZHAO Kai-hong, FU Hong-tao, TIAN Bo, WANG Tong, ZHANG Fang. Value of serum Tau protein in assessing brain injury in premature infants and the recent prognosis [J]. Chinese Journal of Child Health Care, 2022, 30(10): 1153-1157.
[10]	XIAO Chuan-liu. Association between serum levels of fat-soluble vitamins and severity of atopic dermatitis in children [J]. Chinese Journal of Child Health Care, 2021, 29(7): 791-793.
[11]	DONG Ming-ying, TAO Bing-tong. Correlation of leukotriene C4 synthase gene polymorphism with susceptibility and efficacy of respiratory syncytial virus bronchiolitis [J]. Chinese Journal of Child Health Care, 2021, 29(5): 545-549.
[12]	WEI Hong, SONG Xi-yuan, LIU Xue, CAO Hui-ling, KONG Ling-ling, WANG Xu. Meta-analysis on the associations of reduced folate carrier A80G polymorphism with neural tube defects [J]. journal1, 2020, 28(7): 774-779.
[13]	ZHANG Qi-yi, WU Min, WANG Jia-qi, JIA Wan-ru, SHEN Jie, ZHANG Xiao-yan, LIN Jing. Correlation between salivary cortisol levels with sleep quality and severity of clinical symptoms in children with postural tachycardia syndrome [J]. journal1, 2020, 28(4): 379-384.
[14]	XU Qing-rong, SONG Li, ZHANG Yan. Association between CTLA-4 gene polymorphism with childhood bronchial asthmatic susceptibility and levels of IFN-γ, IL-4 and IgE in peripheral blood [J]. journal1, 2020, 28(1): 32-36.
[15]	ZHAO Xiao-fen, ZHOU Xing, ZHANG Yan, HU Hao, QIU Li-ling, LI Yang-fang. Association between interleukin-1β single nucleotide polymorphisms and sepsis in full-term neonates [J]. journal1, 2020, 28(1): 65-68.