Study of the intestinal microbial diversity in obese children by illumina amplicon sequencing of 16SrDNA Tag

doi:10.11852/zgetbjzz2018-1011

Abstract

Abstract: Objective To study the composition and characteristics of intestinal microorganisms in obese children by illumina amplicon sequencing of 16SrDNA Tag, in order to provide basis for further related research. Methods Totally 59 obese children and children with normal weight were enrolled in this study, and their feces were collected.16SrDNA method was used to detect the composition and difference of intestinal flora in different groups. Results There was no significant difference on intestinal microbial diversity between obese and normal weight children(P>0.05), but significant differences on distribution and quantity were found(P<0.05).At the phylum level, Firmicutes, Bacteroidetes and Acidobacteria were the most abundant flora in children′s intestinal microbial community.The number of Verrucomicrobia in normal weight group was significantly higher than that in obese group.At the genus level, it was found that the number of Dorea was significantly higher in obese group than that in normal weight group, while the number of Akkmermansia was significantly higher in normal weight children. Conclusion The change of intestinal flora distribution pattern and quantity is closely related to the occurrence of obesity.

Key words: childhood obesity, intestinal microbial, 16SrDNA amplicon sequencing

摘要： 目的通过16SrDNA测序研究肥胖儿童肠道微生物的组成及变化特点,为后期相关研究奠定基础。方法于2017年4月-2018年4月选取肥胖儿童及正常体重儿童共59例,收集粪便,采用16SrDNA扩增子测序的方法检测儿童肠道菌群组成。结果肥胖组儿童和正常体重组儿童的肠道微生物多样性差异无统计学意义(P>0.05),但分布规律和数量差异有统计学意义(P<0.05)。在门水平中厚壁菌门、拟杆菌门、放线菌门是儿童肠道微生物群落中最丰富的菌群。其中疣微菌门数量在正常体重组显著高于肥胖组儿童。在属水平,发现多尔氏菌数量在肥胖组儿童肠道内显著高于正常体重组儿童,嗜黏蛋白阿克曼氏菌数量在正常体重组儿童显著高于肥胖组儿童。结论肠道菌群分布规律和数量的改变与肥胖的发生有密切联系。

关键词: 儿童肥胖, 肠道菌群, 16SrDNA扩增子测序

CLC Number:

R723.14

WU Ya, FANG Yuan-yuan, CHEN Yan-hui, YANG Li-qi, WANG Jian-qing. Study of the intestinal microbial diversity in obese children by illumina amplicon sequencing of 16SrDNA Tag[J]. journal1, 2018, 26(12): 1297-1300.

吴亚, 方圆圆, 陈彦辉, 杨琍琦, 王建青. 基于16SrDNA测序的肥胖儿童肠道菌群变化分析[J]. 中国儿童保健杂志, 2018, 26(12): 1297-1300.

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