Abstract: Objective To explore the relationship between rs2241423 polymorphism of MAP2K5 gene and the intervention effect of childhood obesity. Methods In a cluster randomized controlled trial to study the effect of intervention on childhood obesity in China, 382 children aged 8 to 10 in Beijing were selected as subjects.Saliva was collected to detect rs2241423 polymorphism of MAP2K5 gene, and the interaction between gene and intervention on childhood obesity was analyzed. Results There was an interaction between rs2241423 polymorphism of MAP2K5 gene and intervention on the changes of obesity-related indexes such as BMI, BMI Z-score, waist-hip ratio, and body weight in children (P_{for interaction}=0.034, 0.007, 0.039, 0.036). Compared with the control group, children in the intervention groupcarrying each additional G allele at MAP2K5 rs2241423 locus, BMI decreased by -0.36 kg/m² (95%CI:-0.69 to -0.03 kg/m², P=0.034), BMI Z-score decreased by -0.20 (95%CI:-0.35 to -0.06, P=0.007), waist-hip ratio decreased by -0.01 (95%CI:-0.02 to -0.001, P=0.039), and body weight decreased by -0.84 kg (95%CI:-1.63 to -0.06, P = 0.036), respectively. Conclusion Children who carried the G allele at rs2241423 of MAP2K5 gene are more sensitive to intervention and showed more improvement in BMI and BMI Z-score, waist-hip ratio, and body weight after intervention.

Key words: childhood obesity, gene, single nucleotide polymorphism

摘要： 目的 探索MAP2K5基因rs2241423多态性与儿童肥胖干预效果的关系,为未来开展基于遗传背景的个性化干预提供科学依据。方法 在一项研究儿童肥胖干预效果的整群随机对照试验中,选取北京地区382名8~10岁儿童作为研究对象,收集唾液,检测MAP2K5基因rs2241423多态性,并分析基因与干预对儿童肥胖的交互作用。结果 MAP2K5基因rs2241423多态性与干预措施对儿童BMI、BMI Z评分、腰臀比和体重等肥胖相关指标的改变存在交互作用(P_交互分别为0.034、0.007、0.039和0.036)。与对照组相比,干预组儿童在MAP2K5基因rs2241423位点上每多携带一个 G等位基因,BMI下降(-0.36 kg/m²,95%CI:-0.69~-0.03 kg/m²,P=0.034),BMI Z评分降低(-0.20,95%CI:-0.35~-0.06,P=0.007),腰臀比降低(-0.01,95%CI:-0.02~-0.001,P=0.039),体重减少(-0.84 kg, 95%CI:-1.63~-0.06 kg,P=0.036)。结论 在MAP2K5基因rs2241423位点上携带G等位基因的儿童对干预更加敏感,接受干预后可在BMI及 Z评分、腰臀比和体重指标上获得更多改善。

关键词: 儿童肥胖, 基因, 单核苷酸多态性