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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2017, Vol. 22 ›› Issue (10): 1090-1098.

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Effects of compound Baihe shugan anshen on stucture and expressions of GR, BDNF and NPY on prefrontal cortex-edge structure in anxious depression model rats

YANG Qin 1, DU Qing 2, ZHAO Hongqing 3, WANG Yuhong 1,3, HAN Yuanshan 1, YANG Hui 1, MENG Pan 3   

  1. 1 First Hospital of Hunan University of Chinese Medicine, Changsha 410007, Hunan, China；2 Hunan Academy of Chinese Medicine, Changsha 410006, Hunan, China;  3 Hunan University of Traditional Chinese Medicine, Training Bases, Hunan Key Laboratory of Chinese Materia Medica Powder and Innovative Drugs Established by Provincial and Ministry, Changsha 410208, Hunan, China
  • Received:2017-03-17 Revised:2017-04-07 Online:2017-10-26 Published:2017-11-13

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Abstract:

AIM: To investigate the effects of compound Baihe shugan anshen on anxiety depression model rat prefrontal cortex-edge(hippocampus and amygdala) structure and expression of GR/BDNF/NPY.  METHODS: SD rats were randomly divided into 7 groups according to body weight: the anxious depression model group, the positive drug (venlafaxine 6.75 mg/kg) group, the compound Baihe shugan anshen-high, -middle, -low (24.28 g/kg, 12.24 g/kg, 6.12 g/kg) group, the control group and the vehicle group. Chronic restraint stress plus solitary feeding and subcutaneous injection of CORT (30 mg/kg) for 21 d were administered to build the animal model of anxiety depression. At the same time, the positive drug group and compound Baihe shugan anshen group received intragastric administration. The vehicle group received subcutaneous injection of physiological saline plus 5%DMSO. The control group was fed normally. Morphological changes of prefrontal cortex (PFC), hippocampus and amygdala were analyzed by HE staining, and determination of GR, BDNF and NPY by Western blotting.  RESULTS: Compared with the blank control group, glial cells were increased while the neurons were decreased; vertebral neurons were physalides, and nucleus were wither in PFC of anxiety-depression rats. Neurons in hippocampal DG and CA3 area were lost, and the nucleus of neurons in CA3 area was deeply stained. The nucleus of amygdala neurons was fuzzy, the cells were scattered, and the number of cells were decreased significantly. The levels of GR, BDNF and NPY in PFC, hippocampus and amygdala were significantly decreased (P<0.05 or P<0.01). Compared with the model group, prefrontal cortex glial cell proliferation was obviously relieved; cells were arranged in order in the Baihe shugan anshen high dose group. Neurons in hippocampus DG and CA3 were increased and CA3 neurons recovered. The damage of neurons in the amygdala was also reduced. The levels of GR, BDNF and NPY in PFC, hippocampus and amygdala were significantly increased (P<0.05 or P<0.01). CONCLUSION: The anti-anxiety and depression effect of Baihe shugan anshen may be related to the cascade of regulation of GR/BDNF/NPY signal to protect the prefrontal-marginal(hippocampus,amygdala) structural injury. 

Key words: anxious depression, compound Baihe shugan anshen, pefrontal cortex-limbic stucture, GR, BDNF, NPY

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