Anti-aging effects of Kangshuailing ointment and its mechanism

doi:10.12092/j.issn.1009-2501.2018.01.005

Abstract

Abstract:

AIM: To observe the anti-aging effects of Kangshuailing ointment and the possible mechanisms. METHODS: ICR mice were randomly divided into six groups: normal group, model group, positive group, Kangshuailing ointment 1.3, 2.6, 5.2 g/kg group. Aging model was induced by subcutaneously injecting mice with 5% D-galactose. Mice of each group receiced daily administration by gavage for 6 weeks. Thymus index and spleen index were calculated; LPO, LF contents and MAO-B activity in brain tissue were detected by ELISA method; MDA contents, SOD and GSH-Px activities in blood serum were examined by spectrophotometry method; the pathology change of brain tissues were observed using light microscope. RESULTS: Compared with model group, Kangshuailing ointment could significantly increase the thymus and spleen index, reduce the LPO and LF contents, and increase MAO-B activity in brain tissue, remarkably decrease the MDA contents and increase the SOD and GSH-Px activities in blood serum. In addition, Kangshuailing ointment could markedly relieve the pathological lesions induced with D-galactose in brain. CONCLUSION: Kangshuailing ointment has obvious anti-aging effect on aging mice, which may be related to the antioxidation.

Key words: aging, Kangshuailing ointment, antioxidation, D-galactose

CLC Number:

R965.2

LUO Shengyong, DENG Xuefeng, LIU Peng, LI Chengwang, LI Rui. Anti-aging effects of Kangshuailing ointment and its mechanism[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(1): 24-28.

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