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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2018, Vol. 23 ›› Issue (1): 24-28.doi: 10.12092/j.issn.1009-2501.2018.01.005

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Anti-aging effects of Kangshuailing ointment and its mechanism

LUO Shengyong 1, DENG Xuefeng 2, LIU Peng 2, LI Chengwang 1, LI Rui 1   

  1. 1 Anhui Provincial Institute of Medical Science, Hefei 230061, Anhui, China; 2 Anhui Yanshoutang Pharmaceutical Company Ltd, Chizhou 245100, Anhui, China
  • Received:2017-06-02 Revised:2017-09-15 Online:2018-01-26 Published:2018-02-07

Abstract:

AIM: To observe the anti-aging effects of Kangshuailing ointment and the possible mechanisms.  METHODS: ICR mice were randomly divided into six groups: normal group, model group, positive group, Kangshuailing ointment 1.3, 2.6, 5.2 g/kg group. Aging model was induced by subcutaneously injecting mice with 5% D-galactose. Mice of each group receiced daily administration by gavage for 6 weeks. Thymus index and spleen index were calculated; LPO, LF contents and MAO-B activity in brain tissue were detected by ELISA method; MDA contents, SOD and GSH-Px activities in blood serum were examined by spectrophotometry method; the pathology change of brain tissues were observed using light microscope. RESULTS: Compared with model group, Kangshuailing ointment could significantly increase the thymus and spleen index, reduce the LPO and LF contents, and increase MAO-B activity in brain tissue, remarkably decrease the MDA contents and increase the SOD and GSH-Px activities in blood serum. In addition, Kangshuailing ointment could markedly relieve the pathological lesions induced with D-galactose in brain. CONCLUSION: Kangshuailing ointment has obvious anti-aging effect on aging mice, which may be related to the antioxidation.

Key words: aging, Kangshuailing ointment, antioxidation, D-galactose

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