Protective effects of OPG-HSP70 fusion protein on CIA rats

Abstract

Abstract: AIM: To study the protective effects of human osteoprotegerin ( OPG) -Mycobacterium tuberculosis heat shock protein 70 ( HSP70) fusion protein on animal models of the rheumatoid arthritis by the observation of the symptoms of collagen-induced arthritis (CIA) rats.METHODS: The OPG-HSP70 recombinant strain stored in the laboratory were induced by isopropyl B-D-thiogalactopyranoside ( IPTG) and the acquired fusion protein were collected and identified, purificated and dialysis refolded.The experimental rats were divided into OPG-HSP70 group, OPG group, HSP70 group, model group and normal group, and were treated with corresponding protein after preparation.The symptoms and signs of collagen-induced arthritis rats were observed carefully.RESULTS: Compared with the positive control group, the OPG-HSP70 fusion protein group demonstrated that the arthritis index was reduced significantly ( P <0.05);the clear structures of the intra-articular synovials and the bone mineral density were increased ( P <0.05) from articular pathology studies.CONCLUSION: OPG-HSP70 fusion protein shows efficacy in alleviating the symptoms of CIA rats.

Key words: osteoprotegerin, heat shock protein, fusion protein, collagen-induced arthritis

CLC Number:

R318

LIU Shan, YU Dan, ZENG Jin-jin, LI Shen-tao, WANG Wei, LIU Zhen-long, ZHAO Wen-ming. Protective effects of OPG-HSP70 fusion protein on CIA rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(12): 1347-1350.

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