Abstract: AIM: To investigate pharmacokinetics of rhproUK in healthy subjects. METHODS: ELISA and ELISA combined with chromogenic substrated method were used to determinate the plasma concentration of uPA, sc-uPA and plasminogen activity. RESULTS: The ratio of AUC of uPA and sc-uPA is 1∶2.1 ∶3.3 and 1 ∶1.9 ∶2.9 after single administration of rhproUK in the healthy subjects at the dose of 20,40 and 60 mg per person,AUC and C_max increase with dosage increasment, there are no significant difference in CLS、VSS、MRT、t_1/2 and t_max between three groups.The increasment of AUC and C_max in plasminogen activity are more than the increasment proportion of dosage, the ratio of Cmax and AUC is 1∶2.3∶3.9　and 1∶2.9∶4.7, showed non-linear increasment. CLS and Vd reduce with dosage increasement. CONCLUSION: The course of u-PA, sc-uPA in healthy subjects fitted with the linear pharmacokinetics model in the range from 20 to 60 mg per person, But plasminogen activity showed slightly non-linear pharmacokinetics.

Key words: Single chain urokinase-type plasminogen activator, Pharmacokinetics, ELISA, Blood drug concentration