Antiobesity effect of ketotifen in high-carbon hydrate-fat diet induced obese rats

Abstract

Abstract: AIM: To investigate the antiobesity effect and mechanism of ketotifen in high-carbon hydrate-fat diet induced obese rats.METHODS: Male SD rats were randomly divided into three groups:obese group,ketotifen group and normal control group.Rats of obese group and ketotifen group were fed with high-carbon hydrate-fat diet,while the rats of normal control group were fed with basal feed. Rats of ketotifen group were daily given a gavage of ketotifen 0.09 mg/kg for 12 weeks.The body weights were tested and then the Lee's indexes were calculated,The fasting blood glucose(FBG),fasting insulin(FINS) and leptin(LEP), free fatty acid(FFA),riglyceride(TG) and low density lipoprotein cholesterin(LDL-C), interleukin -6(IL-6) and tumor nectosis factor-alpha(TNF-α) were analyzed,UCP2 mRNA in white adipose were measured.RESULTS: Ketotifen reduced body weight and Lee's indexes obviously in obese rats(P<0.05), lowered FBG,FINS and LEP levels and reduced FFA,TG and LDL-C levels significantly(P<0.05).Furthermore,ketotifen could decrease IL-6 and TNF-α levels and increase the UCP2 mRNA expression obviously(P<0.05).CONCLUSION: Ketotifen has antiobesity effects on high-carbon hydrate-fat diet induced obese rats by reduction of inflammation medium and free fatty acid leves,alleviation of hyperinsulinemia and hyperleptindemia and promotion of adipose tissue energy metabolism.

Key words: Ketotifen, Obesity, Mast cell, Inflammation, UCP2

CLC Number:

CHEN Zi-miao,WANG Mei-rong,YE Ting-ting,GU Xue-jiang CHEN Xiong, ZHU Hong, NI Lian-song. Antiobesity effect of ketotifen in high-carbon hydrate-fat diet induced obese rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(1): 23-28.

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