Table of Content

Volume 19 Issue 1
27 January 2014

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Protective effect of total Flavonoid C-glycosides from Abrus mollis extract on fatty liver induced by DL-Ethionine in mice

WANG Yun, CHEN Mi, JIANG Zhen-zhou, WANG Hao, ZHANG Lu-yong

2014, 19(1):  1-7. 

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AIM: To study the effect of total flavonoid C-glycosides from Abrus mollis extract (AME) on lipid dysregulation induced by Ethionine (DL-E).METHODS: ICR mice were randomly assigned to four groups (n=8): Control, DL-E, AME and AME+DL-E groups. Mice were gavaged with AME (200 mg/kg) for 7 days. At day 7, mice of DL-E and AME+DL-E group were gavaged with DL-E (250 mg/kg), other two groups were treated with vehicle control (0.2% CMC-Na). Serum and liver tissues were collected 22 h after administration of DL-E. The contents of hepatic lipids, serum lipids and ALT, AST levels were measured. The expressions of key genes involved in lipid metabolism were also observed by real-time PCR.RESULTS: The results indicated that AME provided significant protection against fatty liver by inhibiting the elevation of serum lipids, serum transaminase, reducing hepatic TG and TC accumulation, ameliorating the severity of pathological changes. Furthermore, AME significantly decreased DL-E-induced SREBP-1, FAS and ACC1 high expressions, which resulting in the lipid homeostasis. The down-regulation of CPT-1α and PPARα that reflect fatty acid metabolism induced by DL-E were reversed by AME treatment.CONCLUSION: The results indicated AME has hepatoprotective effect on Ethionine-induced fatty liver in mice and the effect was mostly due to the regulation on lipid synthesis and fatty acid metabolism.

Research on relationship between the progression of chronic obstructive pulmonary disease and the change of MRP1 function in lung bronchial epithelium

WANG Chen-yin, TAO Xiu-hua, ZHANG Xuan,WANG Dian-lei, CHEN Jin-pei, YANG Li-li, CAO Yin, WANG Shan-shan

2014, 19(1):  8-14. 

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AIM: To study the relationship between different progressions of chronic obstructive pulmonary disease (COPD) and the change of MRP1 function in lung bronchial epithelium.METHODS: Making stimulation with tobacco and papain to establish COPD rat model. According to the principle of random, the rats were divided into the normal group, different course of COPD groups, probenecid control group and inhibitor groups.The inhibitor groups were given the MRP1 inhibitor probenecid with the establishing COPD rat model. The function of lung all the rats were evaluated. The permeability and integrity of the lung epithelial cells of all the rats were measured, except the inhibitor group. The phenolsulfonphthalein concentration in bronchoalveolar lavage fluid (BALF) and plasma was determined and the concentration of in BALF/plasma ratio was calculated to evaluate the COPD lung bronchial epithelial MRP1 function.RESULTS: With the development of disease, the lung functions of the rats in different progression of COPD were gradually reduced.There was a significant difference in 45 days later. With the process of COPD, the ratio of phenolsulfonphthalein concentration in BALF and plasma were gradually reduced, but the lung epithelial cell permeability in each groups had not changed and the integrity of the lung epithelial cells was fine.Compared with the normal groups, lung function of the inhibitor group (30 days) was significantly reduced. There was a significant difference in FEV0.3%/FVC between the different courses of COPD groups and inhibitor groups in 60 days.CONCLUSION: There is a certain correlation between the process of COPD and changes of MRP1 function in lung bronchial epithelial. With the progression of COPD, the function of MRP1 is gradually reduced in lung bronchial epithelium. Inhibiting the function of MRP1 can aggravate the lung function of COPD.

Inhibitory effect of genistein on cervical carcinoma cell through AMPK and mTOR signaling pathway

LI Yong-zheng, CHEN Wei

2014, 19(1):  15-22. 

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AIM: To observe the effect of genistein on growth and apoptosis of cervical carcinoma Caski cell lines , and to explore its potential mechanisms.METHODS: Cervical carcinoma cell lines Hela, SiHa, Caski, HeLa-S3, HeLa229 and C-33A were cultured and treated with genistein at different concentration and at different time points. The effect of genistein on cervical carcinoma cell lines proliferation was studied by means of Sulforhodamine B (SRB), the cell cycle and mitochondrial membrane potential were detected with flow eytometry;RT-PCR and western blot were used to detect the expressions of AMPK and mTOR signaling pathway related gene at mRNA and protein levels. Determination of TSC1/TSC2 association by immunoprecipitation assay.RESULTS: The rank order of genistein potency against cervical carcinoma is Caski>Hela> C-33A > SiHa>HeLa-S3>HeLa229. Genistein completely abolished cell-cycle progression released from double-thymidine-block synchronization and caused a subsequent apoptosis. The data were supported by down-regulation and reduced nuclear translocation of G₁-regulator proteins, including cyclin D1, cyclin E, Cdk4 and Cdk2. Further analysis showed that the mRNA expressions of the G₁-regulator proteins were not modified by Methyl jasmonate, indicating an inhibition of translational but not transcriptional levels. Genistein induced the assembly of tuberous sclerosis complex (TSC1/TSC2), leading to the blockade of cellular protein synthesis through inhibition of protein phosphorylation including mTOR (Ser2448), p70S6K (Thr421/Ser424 and Thr389) and 4E-BP1 (Thr37/Thr46 and Thr70). Furthermore, the AMPK activity was elevated by genistein. Compound C, a selective AMPK inhibitor, significantly reversed genistein-mediated effects suggesting the crucial role of AMPK. Besides, the loss of mitochondrial membrane potential and depletion of mitochondrial content indicated the mitochondrial stress caused by genistein.CONCLUSION: Genistein induces anticancer signaling cascades in a sequential manner. The exposure of cells to genistein induces mitochondrial stress and activation of AMPK that further induces the loss of mitochondrial membrane potential and activates TSC1/TSC2 association. Consequently, the mTOR mediated translational pathways are blocked, leading to G₁ arrest of the cell-cycle and subsequent cell death.

Antiobesity effect of ketotifen in high-carbon hydrate-fat diet induced obese rats

CHEN Zi-miao,WANG Mei-rong,YE Ting-ting,GU Xue-jiang CHEN Xiong, ZHU Hong, NI Lian-song

2014, 19(1):  23-28. 

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AIM: To investigate the antiobesity effect and mechanism of ketotifen in high-carbon hydrate-fat diet induced obese rats.METHODS: Male SD rats were randomly divided into three groups:obese group,ketotifen group and normal control group.Rats of obese group and ketotifen group were fed with high-carbon hydrate-fat diet,while the rats of normal control group were fed with basal feed. Rats of ketotifen group were daily given a gavage of ketotifen 0.09 mg/kg for 12 weeks.The body weights were tested and then the Lee's indexes were calculated,The fasting blood glucose(FBG),fasting insulin(FINS) and leptin(LEP), free fatty acid(FFA),riglyceride(TG) and low density lipoprotein cholesterin(LDL-C), interleukin -6(IL-6) and tumor nectosis factor-alpha(TNF-α) were analyzed,UCP2 mRNA in white adipose were measured.RESULTS: Ketotifen reduced body weight and Lee's indexes obviously in obese rats(P<0.05), lowered FBG,FINS and LEP levels and reduced FFA,TG and LDL-C levels significantly(P<0.05).Furthermore,ketotifen could decrease IL-6 and TNF-α levels and increase the UCP2 mRNA expression obviously(P<0.05).CONCLUSION: Ketotifen has antiobesity effects on high-carbon hydrate-fat diet induced obese rats by reduction of inflammation medium and free fatty acid leves,alleviation of hyperinsulinemia and hyperleptindemia and promotion of adipose tissue energy metabolism.

Effects of Xuebijing for injection on neutrophil TCTP expression in Acinetobacter baumannii sepsis rats

HE Xian-di, YAN Pei-e, ZHOU Qi, WANG Hua-xue

2014, 19(1):  29-32. 

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AIM: To study the effect of Xuebijing for injection on neutrophilic granulocyte expressing translationally controlled tumor protein(TCTP) in Acinetobacter baumannii sepsis rats.METHODS: Forty-two male Wistar rats were randomized into three groups: control group (n=6),sepsis group(n=18),Xuebijing group(n=18). After successfully building sepsis models by intra-peritoneal injecting Acinetobacter baumannii suspension, according to the time of the extracting neutrophilic granulocyte in blood, sepsis group and Xuebijing group were randomly divided into 3 subgroups respectively: 6 h, 12 h and 24 h(n=6). Through the Western-blotting method to detect the expression of neutrophil TCTP. Compare them in each subgroup of sepsis group and Xuebijing group.RESULTS: Compared with the control group, sepsis subgroups of neutrophil TCTP expression were increased (P<0.05). Compared with sepsis subgroups, the expression of TCTP in neutrophil of Xuebijing subgroups were decreased (P<0.05).CONCLUSION: Xuebijing for injection in the treatment of sepsis might be related to the inhibition of neutrophil TCTP expression.

Impact of clinical pharmacists on protocol deviation in anti-cancer drug clinical trial

SHAN Jiao-jiao,TANG Yi-qun,SHI Mei-qi, XIA Guo-hao, WANG Li,FANG Ying,ZHAO Jie

2014, 19(1):  33-37. 

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AIM: To explore the impact of clinical pharmacists on the occurrence of protocol deviation in antineoplastic drug clinical trial.METHODS: Subjects between September 2011 and May 2012 were enrolled in non-intervention group,while intervention group included subjects between June 2012 and February 2013 with pharmaceutical intervention. The incidence of protocol deviation was analyzed and the effects of pharmaceutical intervention were observed.RESULTS: 61.3% cases in non-intervention group occurred protocol deviation including 37.3% major protocol deviation,in contrast, 43.9% cases had protocol deviation including 19.7% major protocol deviation in intervention group. Protocol deviation (P<0.05) and major protocol deviation (P<0.05) were significantly lower in intervention group.CONCLUSION: Clinical pharmacists could effectively reduce protocol deviation in antineoplastic drug clinical trials. Being a part of clinical trials provides clinical pharmacist a new opportunity to work at the department of medical oncology.

Bioequivalence of clopidogrel hydrogen sulfate tablets in healthy Chinese volunteers

YANG Yong-ge, ZHANG Mei, JIANG Nan, SONG Li-xue, XU Xue-ting, DI Xiao-hui, XU Lin, XU Juan,ZHAO Gang-tao

2014, 19(1):  38-43. 

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AIM: To evaluate the bioequivalence of clopidogrel hydrogen sulfate tablets(Areplex and Poland) in healthy Chinese volunteers after a single dosage oral administration.METHODS: A single oral doses of 75 mg clopidogrel hydrogen sulfate tablets(test and reference) were given to 32 healthy Chinese volunteers according to an open randomized crossover design. The concentrations of clopidogrel acid(carboxylic metabolite of clopidogrel) in plasma were determined by LC-MS/MS.With the aid of DAS 2.0, the bioequivalence of the test and reference preparation were calculated by analysis of variance, tow one sided t-test.RESULTS: The pharmacokinetic parameters of the two preparations were as follow:C_max of test drug and reference drug were(1351.1±654.9)ng/mL and (1184.6±607.7) ng/mL,AUC_0-24h were (2642.0±1093.8) ng·h·mL^-1 and (2780.6±1283.1) ng·h·mL^-1,t_1/2 were (3.81±2.54)h and (4.62±2.88) h,t_max were (0.80±0.32) h and (0.95±0.63) h,respectively. The relative bioavailability of the formulation was (101.4±34.8)%.CONCLUSION: It is an accurate,sensitive,rapid and convenient method that can be applied to determine clopidogrel in human plasma. The tested preparation is bioequivalence to the reference preparation.

SanAo Tablet in treating acute bronchitis(wind-cold attacking the lung syndrome):A randomized controlled trial

FAN Mao-rong, WHAN Bing, GAO Jin-zhu, FAN Chang-zheng, ZHANG Qiong

2014, 19(1):  44-47. 

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AIM: To evaluate the efficacy and safety of SanAo Tablet in treating acute bronchitis with the syndrome of wind-cold attacking the lung.METHODS: A multicenter, randomized, double blind, double dummy, controlled trial,non-inferiority was conducted. A total of 240 patients with acute bronchitis were randomly divided into two groups: a treatment group (120 patients) were treated with SanAo Tablet and TongXuanLiFei Oral Liquid Analogue, acontrol group (120 patients) were treated with TongXuanLiFei Oral Liquid and SanAo Tablet Analogue. The therapeutic course of both groups was 7 days.RESULTS: For the clinical effective and curative rate, the treatment group obtained better effects than control group(P<0.05).For the effective and curative rate of TCM Syndrome, the treatment group showed a better clinical effect(P<0.05).SanAo tablet had significantly relieved the symptoms of cough and spitting sputum,compared with TongXuanLiFei Oral Liquid, SanAo tablet showed a better clinical effect(P<0.05).Onset time of cough and spitting sputum: there is no significant difference between two groups.The change amount of TCM syndrome total integral before and after treatment is higher in SanAo Tablet group than in TongXuanLiFei Oral Liquid group(P<0.05).CONCLUSION: SanAo Tablet is effective and safe in treating wind-cold attacking the lung pattern of acute bronchitis.

Effects of pre-injection of Dexmedetomidine on safety during Etomidate induction of general anesthesia

ZHAO Gang, CAI Jian-ming, YU Mi-ling, LU Mu

2014, 19(1):  48-53. 

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AIM: To observe the effect pre-injection of Dexmedetomidine on safety during Etomidate induction of general anesthesia.METHODS: Eighty patients with ASA I-Ⅱ scheduled for head or neck surgery were randomly assigned to 4 groups:A(n=20),B(n=20),C(n=20),D(n=20).Group A,B received Dexmedetomidine 1 μg/kg intravenous injection and the whole dose was given within 10 minutes.Group C,D received equal volume of normal saline as Group A intravenously without Dex. Anesthesia was induced with Sufentanil 0.5 μg/kg,Atracurium 0.6 mg/kg and Etomidate 0.2 mg/kg in group A,C .Anesthesia was induced with propofol 1.5 mg/kg in group B,D.All other induction and maintenance drugs were same for two groups.HR,SBP,DBP,BIS were recorded at the time points of pre-administration of Dexmedetomidine (T₀,base value),l min after administration(T₁),10 min after administration(T₂),pre-intubation(T₃),intubation(T₄),l min after intubation(T₅),3 min after intubation(T₆),10 min after intubation(T₇).SpO₂, Ramesay scale were recorded at the time points of T₀ and T₂.RESULTS: After Dexmedetomidine was given,SpO₂ decreased (P<0.01) and Ramesay scale increased in group A and group B(P<0.01).There was no obvious change in group C,D(P>0.05). Compared with the base value,SBP and DBP of each group was decreased at T₃ (P<0.05); compared with the base value,SBP,DBP,and HR of Group C increased obviously with intubation (P<0.05),and HR remained higher than group A and group B after intubation(P<0.01);Compared with the group C,SBP,DBP,and HR of group A and group B were lower at T₄ and T₅(P<0.05).Compared with the group B,SBP and NBP of group A were higher at T₄ (P<0.05). SBP,DBP,and HR at T₅-T₇ were not signficantly different between group A and group B(P>0.05). Compared with the base value,BIS of group A and group B were decreased at T₂ (P<0.01), BIS of group A,B,D were lower than group C at T₅ (P<0.05).CONCLUSION: A single dose of Dexmedetomidine can effectively reduce cardiovascular responses to tracheal intubation during induction of general anaesthesia. Etomidate remain better instantaneous cardiovascular response to laryngoscope and intubation than Propofol and is similar with Propofol in inhibiting the stress response after tracheal intubation during general anaesthesia.

The control research of escitalopram with or without eszopiclone on the treatment of generalized anxiety disorder with insomnia

LI Ya-ling, YE Jian-fei, LI Ting , LI Guo-rong

2014, 19(1):  54-57. 

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AIM: To observe the efficacy and safety of escitalopram with or without eszopiclone on the treatment of generalized anxiety disorder with insomnia patients.METHODS: 100 patients who met the CCMD-3 criteria for generalized anxiety disorder with insomnia were randomly divided into the experimental group (n=52) and the control group (n=48). The experimental group were treated with escitalopram combining with eszopiclone, and the control group with escitalopram monotherapy. The trial lasted 8 weeks. The anxiety status and efficacy was evaluated with HAMA at the baseline,1st, 2nd, 4th, 6th and 8th weekend respectively, the insomnia status with SDRS, and the safety with TESS and laboratory examination.RESULTS: The HAMA scores of the experimental group and the control group declined significantly by the end of 1st week (P＜0.01)and the end of 2nd week (P＜0.01)respectively, and the reduction of HAMA scores of the experimental group was more significant than the control group at the end of 1st, 2nd, 4th and 6th weekend (P＜0.05), while there was no significant difference between the two groups at the end of 8th weekend (P>0.05). The SDRS scores of the experimental group declined significantly by the end of 1st week (P＜0.01), and the reduction of SDRS scores of the experimental group was more significant than the control group at the end of 1st, 2nd, and 4th weekend (P＜0.01). Side events happened more easily in the experimental group than in the control group such as headache, mouth pain, dry mouth, nausea and drowsiness(P＜0.05) .CONCLUSION: Escitalopram with eszopiclone on the treatment of generalized anxiety disorder with insomnia patients contribute to a rapid improvement in insomnia and anxiety, but the side effects are relatively obvious.

Effect of different doses of ketamine on separation anxiety and emergence agitation in pediatrics undergoing PCNL under sevoflurane general anesthesia

XIA Ju-rong,DU Zhong-ju,WANG Bo,ZHANG Hua ,TIAN Hong-yi

2014, 19(1):  58-61. 

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AIM: To investigate the preventive effect of ketamine with different doses on separation anxiety and emergence agitation (EA) after sevoflurane anesthesia in children for PC- NL (percutaneous nephrolithotomy lithotripsy) surgery.METHODS: Eighty children, ranging in age from 2-8 years old, undergoing PCNL surgery were randomly allocated to one of the 4 groups: group C received normal saline, group K0.5, K0.75 and K1 received ketamine 0.5 mg/kg, 0.75 mg/kg or 1.0 mg/kg intravenously before entering the operating room respectively. Children in four groups received conventional sevoflurane for maintain anesthesia. The separation anxiety score before induction was evaluated. Extubation time, post-anesthesia care unit stay time, postoperative nausea and vomiting, emergence agitation, and pain (the modified Children's Hospital of Eastern Ontario Pain Scale,Modified CHEOPS) were assessed.RESULTS: The preoperative separation anxiety scores in group K0.75 and group K1 were significantly lower than those in group K0.5 and group C(P<0.05). Time of extubation and post-anesthesia care unit stay in group K1 was significantly lower than that three groups(P<0.05). The incidence of EA and Modified CHEOPS were significantly higher than those three-ketamine groups(P<0.05),but there was no statistically significant differences among three ketamine groups(P>0.05).CONCLUSION: Ketamine 0.75 mg/kg administered before entering the operating room reduced separation anxiety, postoperative pain and incidence of EA without delay in recovery.

Effect of CRRT on nitric oxide synthase and procalcitonin in systemic inflammatory response syndrome and sepsis patients

ZENG Ai-ying, DAI Mu-sen, WANG Xiao-ping, CHEN Beng-dun, CHEN Min, CHEN Bing-xing, SUN Hong

2014, 19(1):  62-66. 

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AIM: The purpose of this study is to evaluate the efficacy of the Continuous Renal Replacement Therapy (CRRT) on systemic inflammatory response syndrome (SIRS) and Sepsis and the influence on nitric oxide synthase (NOS) and procalcitonin (PCT), to provide new ideas on the CRRT of SIRS.METHODS: We collected the SIRS and Sepsis cases in Department of emergency ICU, internal medicine ICU and surgical ICU of our hospital, recorded changes (before CRRT, 12, 48, 72 h purification treatment), in vital signs, oxygenation index (PaO₂/FiO₂), blood biochemistry, blood routine, blood gas analysis and APACHE II score. NOS was detected by colorimetric method, and PCT was detected by immune chemiluminescence method.RESULTS: After 24, 48, 72 h of CRRT treatment, blood biochemical and blood indexes were significantly improved, and the values of NOS and PCT were all significantly lower than the values of treatment before. The difference was statistically significant (P<0.05).CONCLUSION: CRRT can improve the clinical symptoms of patients with SIRS/Sepsis by removing the damage factor of NOS and PCT.

Basic research of microRNA-34 for cancer therapy

LI Zheng, XIANG Kai-ming, PENG Shu-ping, LI Gui-yuan

2014, 19(1):  67-73. 

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MicroRNA-34(miR-34) inhibits many target genes involved in multiple oncogenic signaling pathways. The broad activity of miR-34 includes induction of cell cycle arrest and apoptosis, inhibition of tumor stem cells and metastasis and the anti-chemoresistance. There are increasing evidence suggesting that miR-34 plays a crucial role in cancer diseases and may be a potential therapeutic target. This review summarizes the biological function and molecular mechanisms of the miR-34 family, pharmacologies in animal tumor models, and reveals the possibility of miR-34 replacement therapy for the clinical treatment of tumor.

Advances in the study of post-transplantation diabetes mellitus resulting from tacrolimus via calcineurin/NFAT signaling

WANG Jiang-lin, ZUO Xiao-cong, YANG Meng, ZHOU Ling-yun

2014, 19(1):  74-81. 

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Tacrolimus (FK506), a calcineurin phosphatase inhibitor, is the base anti-rejection drug after organ transplantation. Post-transplant diabetes mellitus (PTDM) is a complication after a solid organ transplant, and its incidence is widely variable, ranging from 2% to 53%. Tacrolimus is an important risk of PTDM. Tacrolimus inhibits the dephosphorylation of the nuclear factor of activated T-cells (NFATc) in cytoplasm by inhibiting the function of the calcineurin. Then the transport of NFATc into the nucleus and combination with the target DNA are impaired. As a consequence, the promoter cannot activate the transcription of related genes, which decrease the level of insulin by affecting beta cell proliferation, apoptosis and the secretion of insulin and increase the insulin resistance in the target tissue of insulin, leading to the development of PTDM.

Progress in research of phase Ⅱ metabolisms and their enzymes

SHI Shu-ya, WANG Lian-sheng

2014, 19(1):  82-89. 

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Phase Ⅱ drug-metabolizing reaction, characterized by the conjugation reaction, is an important way of body drug disposition. Glucuronidation, acetylation, methylation, glutathione conjugation reaction, and sulphation represent different conjugation reactions and are catalyzed by some similar genes or gene superfamily respectively. All these phase Ⅱ metabolic reactions have their own different characteristics and functional significance. Drug induced significant change of phase Ⅱ drug metabolizing enzyme activity may lead to clinical drug interactions. The genetic polymorphisms of phase Ⅱdrug metabolizing enzymes will affect the metabolism of endogenous substances, which may lead to the increased incidence of certain diseases, and these polymorphisms also affect the metabolism of exogenous substances, causing toxicity or changes in drug efficacy.

Advance in study on tacrolimus pharmacodynamics

YANG Meng, ZUO Xiao-cong,WANG Jiang-lin,CHEN Qing-Jie

2014, 19(1):  90-95. 

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Tacrolimus is a kind of calcineurin inhibitor, mainly suppressing the transcription of cytokines IL-2, IL-3, IL-4, IL-5, IFN-γ, TNF-α, GM-CSF and expression of IL-2, IL-17 receptor. In addition, tacrolimus shows remarkable immunosuppressive effects in the prevention and treatment of organ transplant rejection in clinical and animal experiments. Its major adverse reaction includes nephrotoxicity, neurotoxicity, hypertension, posttransplantation diabetes mellitus,gingival hyperplasia, and so on.

Frequent questions and attentions of applying add-on design to clinical study of Chinese medicine

LIANG Wei-xiong

2014, 19(1):  96-100. 

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An add-on design used in a placebo-controlled clinical trial study, means designing for all the subjects to receive a tested drug or placebo in addition to standard therapy. In recent years, since more add-on studies have been initiated for clinical trials of Chinese medicine, it is necessary to make the objective, frequent questions and attentions more clearer.

Advances in the study of Burkholderia cepacia complex

WANG Yu-ping, LI Xiao-ning, ZHANG Ying-ying

2014, 19(1):  101-104. 

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The Burkholderia cepacia complex(Bcc) is a group of nonfermenter aerobic gram-negative bactetia also an important opportunistic pathogens which easily causes infections in cystic fibrosis patients and chronic granulomatous disease. All of this result in patients asymptomatic carriage, chronic infection or cepacia syndrome. Besides CF and CG, cases infected of Burkholderia cepacia complex are growing because antimicrobial agents abuse, it induces pneumonia, blood poisoning, wound infected, deeping abscess and urinary tract infections. The advances in the study of the Burkholderia cepacia complex in genomovars, epidemiology characteristic identification approach,virulent factor and resistant mechanism are reviewed in this paper.

The research progress of plant estrogen genistein in osteoporosis treatment

LIN Zhong, SUN Yuan, DU You-gong, YANG Jian-miao, LIN Jian-qun

2014, 19(1):  105-111. 

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Based on the correlative materials of recent 10 year searched from the data base of Google Scholar and Science Direct,the mechanism of anti-osteoporosis, the pharmacological activity of anti-osteoporosis, the pharmacokinetics, drug safety of genistein had been briefly summarized overlaying multiple perspectives. The binding force of genistein to the estrogen receptor α is greater than that to the estrogen receptor β and the primary receptor of its pharmacological activity of anti-osteoporosis is estrogen receptor α.Whatever in vitro or in vivo, all the reports below indicate the anti-osteoporosis ability of genistein is surprising. The pharmacology activity of genistein includes inhibited the ability of bone marrow macrophage translated into to osteoclast , inhibited the proliferation ability of osteoclast , induced the apoptosis of osteoclasts, and promoted the proliferation ability of osteoblast.Therapeutic efficacy of anti-osteoporosis is definite, but the toxic and side effect is less than diphosphonate and teriparatide. Geniste has the potential of becoming the clinical first-line drug in treatment of osteoporosis in the future.

Progress of retinoids in the treatment of epidermodysplasia verruciformis

LIAO Wei-jian, HUANG Jian-qing

2014, 19(1):  112-115. 

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Retinoids are compounds of similar structures to natural vitamin A. Till now, there are only a few reports that cover Epidermodysplasia verruciformis treated with retinoids. Certain clinical efficacy of Retinoids in dealing with Epidermodysplasia verruciformis has been proved yet the number of the cases is not enough and requires further systematical comparable research.

Development of maintenance treatment about rheumatoid arthritis in remission

LI Xiao-xin, XU Liang

2014, 19(1):  116-120. 

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Rheumatoid arthritis (RA) is a kind of complex and multi-system autoimmune disease with the main characteristics of the inflammation in joints .About the drug use of RA in remission is always a difficult and hot problem in rheumatology academia for a long time. In recent years, with the maturity of DMARDs and the rapid development of biological agents, the maintenance treatment and drug selection in RA is more flexible. New breakthrougs about curative effect, prognosis and the control of adverse events have been made in the past few years. Now the maintenance treatment of rheumatoid arthritis in remission was reviewed.