关键词: 鲍曼不动杆菌, 抗菌药物, 质粒介导, 喹诺酮, 基因

Abstract:

AIM: To investigate drug resistance of Acinetobacter baumanii strains isolated in the hospital from 2018 to 2019, and explore plasmid-mediated delivery of quinolones resistance genes (PMQR) in Acinetobacter baumannii, to provide references for the clinical therapy of infections and to analysis the genetic mechanisms of resistance by aid of molecular methods．METHODS: Bacteria were identified by VITEK-2 compact System, the results of antibiotics susceptibility test was determined by using microdilution method MIC．We amplified Genes of qnrA,B,C,D, S,aac(6’)-Ib and qepA on plasmid by PCR, and PCR products of each resistant gene for sequencing analysis wrer random selected. We analyzed the results of antibiotics susceptibility of 152 clinical isolates based on WHONET5.6 software．RESULTS: In the past two years, 152 strains of Acinetobacter baumanii have been detected from clinical specimens. The antibiotics resistance rate of Acinetobacter baumanii isolates was an increasing trend year by year．PCR indicated that some strains of Acinetobacter baumanii have products of aac(6’)-Ib and qnrB gene, 29.6%（45/152） strains carrying aac(6’)-Ib and 1.3% (2/152) strains carrying qnrB, there were no products of qnrA,C,D,S and qepA genes on plasmid．CONCLUSION: The current drug-resistance rate among Acinetobater baumannii strains in Hefei to common antibiotics is high, and PMQR carrying rate is lvw．

Key words: Aeinetobacter baumanii, antimierobial agent, Plasmid-mediated, Quinolones, gene