天麻素通过调控PI3K信号通路并降低活性氧水平减轻6-羟基多巴胺诱导的PC12细胞凋亡

doi:10.12092/j.issn.1009-2501.2026.09.001

中国临床药理学与治疗学 ›› 2026, Vol. 31 ›› Issue (9): 1153-1161.doi: 10.12092/j.issn.1009-2501.2026.09.001

• 基础研究 • 下一篇

天麻素通过调控PI3K信号通路并降低活性氧水平减轻6-羟基多巴胺诱导的PC12细胞凋亡

张鑫宇¹(), 温绍文¹, 赵斐², 吴丽丽¹, 张炅璐¹, 房泽海², 刘红杰¹^,*()

1. 暨南大学中医学院，广州 510632，广东
2. 淄博职业技术大学护理学院，淄博 255300，山东

收稿日期:2025-08-15 修回日期:2026-01-20 出版日期:2026-09-26 发布日期:2026-10-08
通讯作者: 刘红杰 E-mail:yxjcsbt@163.com;hongjie_liu@jnu.edu.cn
作者简介:张鑫宇，博士在读，从事中医脑病学研究。E-mail：yxjcsbt@163.com
基金资助:
国家自然科学基金面上项目（82074306）；第五批全国中医临床优秀人才研修项目（国中医药人教函[2022]239号）

Gastrodin attenuates 6-hydroxydopamine-induced apoptosis in PC12 cells by modulating the PI3K signaling pathway and reducing reactive oxygen species levels

Xinyu ZHANG¹(), Shaowen WEN¹, Fei ZHAO², Lili WU¹, Jionglu ZHANG¹, Zehai FANG², Hongjie LIU¹^,*()

1. School of Traditional Chinese Medicine, Jinan University, Guangzhou 510632, Guangdong, China
2. School of Nursing, Zibo Polytechnic University, Zibo 255300, Shandong, China

Received:2025-08-15 Revised:2026-01-20 Online:2026-09-26 Published:2026-10-08
Contact: Hongjie LIU E-mail:yxjcsbt@163.com;hongjie_liu@jnu.edu.cn

摘要/Abstract

摘要：

目的: 探讨天麻及其主要活性成分天麻素对帕金森病的潜在治疗机制，重点分析其在细胞凋亡、氧化应激及磷脂酰肌醇-3-激酶（PI3K）/RAC丝氨酸/苏氨酸蛋白激酶（Akt）/丝氨酸/苏氨酸蛋白激酶mTOR（mTOR）信号通路中的作用。方法: 采用 6-羟基多巴胺（6-OHDA）诱导的PC12细胞损伤模型，通过体外实验评估天麻素对细胞凋亡、细胞内活性氧水平及PI3K/Akt/mTOR信号通路相关蛋白表达的影响。结果: 实验结果表明，天麻素可显著改善6-OHDA诱导的PC12细胞凋亡，并对PI3K信号通路活性产生调控作用。此外，天麻素能够有效降低细胞内活性氧水平，该作用不依赖于PI3K信号通路的调控。结论: 天麻素可能通过降低细胞内活性氧水平并调控 PI3K/Akt/mTOR 信号通路的活性，协同发挥神经保护作用，从而减轻6-OHDA诱导的细胞凋亡，为其在帕金森病防治中的应用提供实验依据。

关键词: 天麻, 天麻素, PI3K/Akt/mTOR信号通路, 细胞凋亡

Abstract:

AIM: To investigate the potential therapeutic mechanisms of Gastrodia elata and its main active component gastrodin (GSD) in Parkinson's disease (PD), focusing on apoptosis, oxidative stress, and the PI3K/Akt/mTOR signaling pathway. METHODS: A 6-hydroxydopamine (6-OHDA)-induced PC12 cell damage model was used to evaluate the effects of GSD on cell apoptosis, intracellular reactive oxygen species (ROS) levels, and the expression of PI3K/Akt/mTOR signaling pathway-related proteins in vitro. RESULTS: GSD significantly attenuated 6-OHDA-induced apoptosis in PC12 cells and modulated the activity of the PI3K signaling pathway. In addition, GSD effectively reduced intracellular ROS levels, and this effect was independent of PI3K pathway regulation. CONCLUSION: Gastrodin may exert neuroprotective effects by reducing intracellular ROS levels and modulating the PI3K/Akt/mTOR signaling pathway, thereby alleviating 6-OHDA-induced apoptosis in PC12 cells. These findings provide experimental evidence supporting the potential application of GSD in PD therapy.

Key words: Gastrodia elata Blume, Gastrodin, PI3K/Akt/mTOR signaling pathway, cell apoptosis

中图分类号:

张鑫宇, 温绍文, 赵斐, 吴丽丽, 张炅璐, 房泽海, 刘红杰. 天麻素通过调控PI3K信号通路并降低活性氧水平减轻6-羟基多巴胺诱导的PC12细胞凋亡[J]. 中国临床药理学与治疗学, 2026, 31(9): 1153-1161.

Xinyu ZHANG, Shaowen WEN, Fei ZHAO, Lili WU, Jionglu ZHANG, Zehai FANG, Hongjie LIU. Gastrodin attenuates 6-hydroxydopamine-induced apoptosis in PC12 cells by modulating the PI3K signaling pathway and reducing reactive oxygen species levels[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2026, 31(9): 1153-1161.

图/表 5

图 1

Fig.1 Effect of gastrodin on apoptosis of PC12 cells A: cell viability of PC12 cells treated with different concentrations of 6-OHDA. B: cell viability of PC12 cells treated with different concentrations of gastrodin (GSD). C: effects of gastrodin at different concentrations on the viability of PC12 cells exposed to 60 μmol/L 6-OHDA. Cell viability in panels (A–C) was assessed using the MTT assay. D–E: flow cytometric analysis of apoptosis. F: Western blot analysis of Bax, BCL-2, and Caspase-3 protein expression in PC12 cells. G–I: quantitative analysis of Bax, BCL-2, and Caspase-3 protein levels. Protein bands were quantified by densitometric analysis and normalized to GAPDH. Data are presented as mean±SEM. For panels (A–C), n=5–6; for panels (F–I), n=3. *P<0.05, **P<0.01, ***P<0.001.

图 2

Fig.2 Effects of gastrodin on apoptosis of PC12 cells while adding LY294002 A–B: representative flow cytometric analysis of cell apoptosis. Data are presented as mean±SEM, n=3. ***P<0.001.

图 3

Fig.3 Effects of gastrodin on Bax, BCL-2 and Caspase-3 protein expression of PC12 cells while adding LY294002 A: Western blot analysis of Bax, BCL-2, and Caspase-3 protein expression in PC12 cells. B–D: quantitative analysis of Bax, BCL-2, and Caspase-3 protein levels. Protein bands were quantified by densitometric analysis and normalized to GAPDH. Data are presented as mean±SEM, n=3. **P<0.01, ***P<0.001.

图 4

Fig.4 Effect of gastrodin on the protein expression of PI3K, Akt and mTOR A: Western blot analysis of PI3K, Akt, and mTOR protein expression in PC12 cells. B–D: quantitative analysis of PI3K, Akt, and mTOR protein levels. Protein bands were quantified by densitometric analysis and normalized to GAPDH. E–F: representative flow cytometric fluorescence intensity profiles. Data are presented as mean±SEM, n=3. ***P<0.001.

图 5

Fig.5 The level of ROS production under the intervention of gastrodin A: Western blot analysis of PI3K, Akt, and mTOR protein expression in PC12 cells. B–D: quantitative analysis of PI3K, Akt, and mTOR protein levels. Protein bands were quantified by densitometric analysis and normalized to GAPDH. Data are presented as mean±SEM, n=3. *P<0.05, **P<0.01, ***P<0.001.

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天麻素通过调控PI3K信号通路并降低活性氧水平减轻6-羟基多巴胺诱导的PC12细胞凋亡

Gastrodin attenuates 6-hydroxydopamine-induced apoptosis in PC12 cells by modulating the PI3K signaling pathway and reducing reactive oxygen species levels

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