活体实验性胰腺炎动物模型的选择

中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (2): 125-129.

活体实验性胰腺炎动物模型的选择

王念林, 陈垦¹, 王晖²

广东医学院附属医院消化内科, 湛江 524000, 广东;
¹广东药学院临床医学系, 广州 510224;
²广东医学院药理教研室, 湛江 524023, 广东

收稿日期:2003-07-30 修回日期:2003-10-17 出版日期:2004-02-26 发布日期:2020-11-16
通讯作者: 陈垦,男, 主任医师, 硕士生导师, 主要从事胃肠道疾病模型和治疗的研究。Tel:020-34074970 　E-mail: chenkenck@tom.com
作者简介:王念林, 男, 硕士研究生, 从事胰腺炎疾病动物模型和治疗的研究。E-mail:wnl7712@163.com;王晖, 男, 副教授, 硕士生导师, 主要从事皮肤和消化道粘膜药理学的研究。
基金资助:
广东高校自然科学研究项目(NO.Z02044)

Choice of animal models of experiment pancreatitis in vivo

WANG Nian-Lin, CHEN Ken¹, WANG Hui²

Department of Digestion, Affiliated Hospital, Guangdong Medical College, Zhanjiang 524000, Guangdong, China;
¹Department of Clinical Medicine Science, Guangdong College of Pharmacy, Gungzou 510224, China;
²Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong, China

Received:2003-07-30 Revised:2003-10-17 Online:2004-02-26 Published:2020-11-16

摘要/Abstract

摘要： 为探讨胰腺炎的发病机制、病因、病理生理及治疗方法, 已建立了众多的活体胰腺炎动物模型。由于活体胰腺炎模型具有操作相对简单、容易控制、便于观测全身变化指标和利于治疗研究等优点, 目前应用较多, 而正确选择一种既适合实验设计目的,又简单易行, 重复性好的模型是进行胰腺炎研究的基本条件之一。

关键词: 胰腺炎, 动物模型, 发病机制, 核转录因子-κB

Abstract: Various animal models of the experiment pancreatitis in vivo were described in this paper. Although these models were widely applied nowadays, the appropriate choice of available model from them should be suit to experimental design and duplicated easily.

Key words: pancreatitis, animal model, pathogenesis, nuclear factor-{kappa}B

中图分类号:

R965.1

王念林, 陈垦, 王晖. 活体实验性胰腺炎动物模型的选择[J]. 中国临床药理学与治疗学, 2004, 9(2): 125-129.

WANG Nian-Lin, CHEN Ken, WANG Hui. Choice of animal models of experiment pancreatitis in vivo[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(2): 125-129.

参考文献

1 Foitzik T, Faulhaber J, Hotz HG, Kirchengast M, Buhr HJ.Endothelin mediates local and systemic disease sequelae insevere experimental pancreatitis[J]. Pancreas, 2001;2(3):248-54
2 Mayer JM, Laine VJ, Kolodziej S, Nevalainen TJ, Beger HG.Acute pancreatitis in transgenic mice expressing human group IIA phospholipase A2[J]. Pancreas, 2002;25(2):188-91
3 Gray KD, Simovic MO, Chapman WC, Blackwell TS, Christman JW, WashingtonMK, et al. Systemic nf-kappaB activation in a transgenic mouse model of acute pancreatitis[J]. J Surg Res, 2003 ;110(1):310-4
4 LampelM, Kern HF.Acute interstitial pancreatitis in the rat induced by excessive doses of a pancreatic secretagogue[J]. Virchows Arch(A), 1977 ;373(2):97-117
5 Tani S, Otsuki M, Itoh H, Fujii M, Nakamura T, Oka T, et al. Histologic and biochemical alterations in experimental acute pancreatitis induced by supramaximal caerulein stimulation[J]. Int J Pancreatol, 1987;2(5-6):337-48
6 Abdo EE, Coelho AM, Montagnini AL, Kubrusly MS, Leite KR, Sampietre SN, et al. Simplified model of induction of experimental acute pancreatitis with a supra-maximal dose of cerulein[J]. Rev Hosp Clin Fac Med Sao Paulo, 1994 ;49(5):204-7
7 Harvey MH, Wedgwood KR, Reber HA.Treatment of acute pancreatitis with beta-adrenergic agonist drugs[J]. Surgery, 1987;102(2):229-34
8 Kinnala PJ, Kuttila KT, Gronroos JM, Havia TV, Nevalainen TJ, Niinikoski JH.Splanchnic and pancreatic tissue perfusion in experimental acute pancreatitis[J]. Scand J Gastroenterol,2002;37(7):845-9
9 Aho HJ, Koskensalo SM, Nevalainen TJ.Experimental pancreatitis in the rat.Sodium taurocholate-induced acute haemorrhagic pancreatitis[J]. Scand J Gastroenterol, 1980;15(4):411-6
10 徐家裕.重症急性胰腺炎的研究[J]. 新消化病学杂志,1997 ;5(特刊6):1-2
11 He ZJ, Alho H, Harmoinen A, Ahola T, Nordback I.Extrapancreatic multiorgan injury in a severe sublethal acute pancreatitis model[J]. Int J Surg Investig, 1999;1(3):177-84
12 SchwarzM, Thomsen J, Meyer H, Buchler MW, Beger HG.Frequency and time course of pancreatic and extrapancreatic bacterial infection in experimental acute pancreatitis in rats[J]. Surgery, 2000;127(4):427-32
13 Nevalainen TJ, Seppa A.Acute pancreatitis caused by closed duodenal loop in the rat[J]. Scand J Gastroenterol, 1975;10(5):521-7
14 Redha F, Uhlschmid G, Ammann RW, Freiburghaus AU.Injection of microspheres into pancreatic arteries causes acute hemorrhagic pancreatitis in the rat:a new animal model[J]. Pancreas, 1990;5(2):188-93
15 Wang D, Jin D, Wu Z, Zou W, Xu D, Zheng Z.Therapeutic effects of human interleukin 10 gene transfer on severe acute pancreatitis in rats, an experimental study[J]. NatlMed J China,2002;82(12):844-7
16 秦仁义, 吴在德, 邹声泉, 肖雪明, 裘法祖.新型的胆源性胰腺炎动物模型[J]. 中华实验外科志, 1998 ;15(1):70-1
17 Niederau C, Luthen R, Niederau MC, Grendell JH, Ferrell LD.Acute experimental hemorrhagic-necrotizing pancreatitis induced by feeding a choline-deficie, ethionine-supplemented diet:Methodology and standards[J]. Eur Surg Res, 1992 ;24(Suppl 1):40-54
18 Lu SC, Gukovsky I, Lugea A, Reyes CN, Huang ZZ, Chen L.Role of S-adenosylmethionine in two experimental models of pancreatitis[J]. FASEB J, 2003 ;17(1):56-8
19 Czako L, Takacs T, Varga IS, Hai DQ, Tiszlavicz L, Hegyi P.The pathogenesis of L-arginine-induced acute necrotizing pancreatitis:inflammatory mediators and endogenous cholecystokinin[J]. J Physiol Paris, 2000;94(1):43-50
20 张云利, 尹浩然, 汤耀卿.过量L-精氨酸致大鼠急性坏死性胰腺炎模型的制备[J]. 中华实验外科杂志, 1998;15(2):186-7
21 Foitzik T, Mithofer K, Ferraro MJ, Fernandez-del Castillo C, Lewandrowski KB, Rattner DW.Time course of bacterial infection of the pancreas and its relation to disease severity in a rodent model of acute necrotizing pancreatitis[J]. Ann Surg,1994;220(2):193-8
22 Ding SP, Li JC, Jin C.A mouse model of severe acute pancreatitis induced with caerulein and lipopolysaccharide[J]. World J Gastroenterol, 2003;9(3):584-9
23 Su SB, Motoo Y, Xie MJ, Miyazono K, Sawabu N.Expression of transforming growth factor-beta in spontaneous chronic pancreatitis in the WBN Kob rat[J]. Dig Dis Sci, 2000;45(1):151-9
24 Sparmann G, Merkord J, Jaschke A, Nizze H, Jonas L, Lohr M.Pancreatic fibrosis in experimental pancreatitis induced by dibutyltin dichloride[J]. Gastroenterology, 1997 ;112(5):1664-72
25 Vera-Portocarrero LP, Lu Y, Westlund KN.Nociception in persistent pancreatitis in rats:effects of morphine and neuropeptide alterations[J]. Anesthesiology, 2003;98(2):474-84
26 Van Laethem JL, Robberecht P, Resibois A, Deviere J.Transforming growth factor beta promotes development of fibrosis after repeated courses of acute pancreatitis in mice[J]. Gastroenterology, 1996 ;110(2):576-82
27 Matsumura N, Ochi K, Ichimura M, Mizushima T, Harada H, Harada M.Study on free radicals and pancreatic fibrosis-pancreatic fibrosis induced by repeated injections of superoxide dismutase inhibitor[J]. Pancreas, 2001 ;22(1):53-7
28 Haber PS, Keogh GW, Apte MV, Moran CS, Stewart NL, Crawford DH.Activation of pancreatic stellate cells in human and experimental pancreatic fibrosis[J]. Am J Pathol, 1999 ;155(4):1087-95
29 王兴鹏, 龚自华, 吴恺, 张汝玲.慢性胰腺炎动物模型的建立[J]. 胰腺病学, 2001 ;1(1):52-3

[1]	王朝义, 宋强, 熊鑫, 王梦远. 肉芽肿性小叶性乳腺炎诊疗进展[J]. 中国临床药理学与治疗学, 2023, 28(8): 910-917.
[2]	张明康, 马彦荣, 靳永文, 周燕, 崔睿睿, 武新安. 药源性急性间质性肾炎的临床研究进展[J]. 中国临床药理学与治疗学, 2023, 28(4): 419-428.
[3]	黄凯歌, 许勤华, 王伟. 血糖、血钙对急性重症胰腺炎患者预后影响的交互作用及预测效能研究[J]. 中国临床药理学与治疗学, 2023, 28(11): 1227-1234.
[4]	黄会珍, 韩磊, 林晓冬, 陈磊. 胰腺炎发病机制相关信号通路研究进展[J]. 中国临床药理学与治疗学, 2023, 28(1): 109-113.
[5]	余伟迪, 宋振顺. 骨髓间充质干细胞通过抑制铁死亡改善大鼠重症急性胰腺炎相关肺损伤[J]. 中国临床药理学与治疗学, 2022, 27(7): 721-728.
[6]	赵正卿, 李雁鹏, 向小霞, 庄建华, 赵忠新. 慢性失眠的发病机制与认知行为治疗[J]. 中国临床药理学与治疗学, 2021, 26(5): 482-486.
[7]	张晓丽, 刘毅 . 瞬时受体电位离子通道与银屑病的研究进展[J]. 中国临床药理学与治疗学, 2021, 26(4): 469-473.
[8]	金晶, 程虹. 线粒体能量代谢参与抑郁症发病的研究进展[J]. 中国临床药理学与治疗学, 2021, 26(10): 1193-1199.
[9]	张琛涵, 聂静云, 辛鹏飞, 柴琛, 李波. 褪黑素与胰腺疾病的研究进展[J]. 中国临床药理学与治疗学, 2020, 25(11): 1309-1314.
[10]	曹志文，胡卫华. 生长激素宫腔灌注治疗薄型子宫内膜的研究[J]. 中国临床药理学与治疗学, 2019, 24(9): 983-988.
[11]	郑琼娜，武俏俏，马哲乐，张贤菲，干方敏，孔庆名. 低分子肝素钠对小鼠实验性肝纤维化干预作用的初步研究[J]. 中国临床药理学与治疗学, 2019, 24(5): 511-516.
[12]	孔令宇，席錾，杨亚勤，马文婷，吴畏，郝同琴. 地塞米松对小鼠急性胰腺炎胰腺组织中Notch信号及凋亡相关基因表达的影响[J]. 中国临床药理学与治疗学, 2019, 24(2): 134-139.
[13]	廖伟，汤玉成，周婷婷，周芙容. miR-423-5p靶向水通道蛋白1对雨蛙肽诱导的急性胰腺炎AR42J细胞损伤的影响[J]. 中国临床药理学与治疗学, 2019, 24(12): 1394-1401.
[14]	陈曦，凌嘉伟，丁嘉欣，江振洲，张陆勇. 他莫昔芬诱导非酒精性脂肪肝的机制研究进展[J]. 中国临床药理学与治疗学, 2018, 23(6): 715-720.
[15]	杨飞云，朱秀英，张建新，杨亚勤. 黑色素瘤缺乏因子2在小鼠急性胰腺炎模型中的表达[J]. 中国临床药理学与治疗学, 2018, 23(1): 51-54.