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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (6): 691-694.

• 研究原著 • 上一篇    下一篇

离体膈肌疲劳模型及其沙美特罗和咖啡因的干预作用

徐旋里, 杨秋火1, 方理本1, 谢强敏1, 沈岳良1   

  1. 杭州市红十字会医院呼吸内科, 杭州310004, 浙江;
    1浙江大学医学院国家药品监督管理局浙江呼吸药物研究实验室, 杭州310031, 浙江
  • 收稿日期:2004-02-13 修回日期:2004-05-20 发布日期:2020-11-22
  • 通讯作者: 杨秋火,男, 教授, 硕士生导师, 从事呼吸生理学、药理学研究。Tel:0571-87217380  E-mail:yangqh86 @163.com
  • 作者简介:徐旋里, 女, 硕士研究生, 主治医师, 从事呼吸系统疾病和药理研究。Tel:0571-85186042-110 E-mai l:hangzhoujb @163.com

Study on the model of diaphragmatic fatigue in vitro induced by hypoxic, hypercapnic acidosis and effects of salmeterol and caffeine

XU Xuan-Li, YANG Qiu-Huo1, FAN Li-Ben1, XIE Qiang-Min, Qiang-Min1, SHEN Yue-Liang1   

  1. Department of Respiratory, Red Cross Society Hospital of Hangzhou, Hangzhou 310031, Zhejian, China;
    1Zhejiang Respiratory Drugs Research Laboratory of State Drugs Administration of China, Zhejiang University, Hangzhou 310031, Zhejian, China
  • Received:2004-02-13 Revised:2004-05-20 Published:2020-11-22

摘要: 目的 建立低氧高碳酸性大鼠离体膈肌疲劳模型, 探讨沙美特罗、咖啡因对此种大鼠膈肌疲劳的作用。 方法 用低氧高二氧化碳混合气造成膈肌疲劳模型, 观察沙美特罗(10-9 ~ 10-6 mol·L-1) 及咖啡因(10-5 ~ 10-3 mol·L-1) 对膈肌收缩力的影响。 结果 混合气降低了5 ~ 120 Hz 所有频率下的张力, 且使峰颤搐张力、维持张力、肌刺张力均下降;沙美特罗10-9 ~ 10-6 mol·L-1 提高了力-频率关系曲线中40 ~ 120 Hz 下的张力;咖啡因10-4 和10-3 mol·L-1明显增强了5 ~ 120 Hz 所有频率下的张力(P <0.01);同时增强了峰颤搐张力、维持张力及肌刺张力。 结论 通低氧高二氧化碳混合气4 min 能够造成低氧高碳酸性大鼠离体膈肌疲劳模型, 沙美特罗和咖啡因对此种大鼠膈肌疲劳有一定的改善作用。

关键词: 沙美特罗, 咖啡因, 膈肌疲劳, 模型

Abstract: AIM: To set up a fatigued diaphragm model in vitro duo to hypoxic, hypercapnic acidosis, and to observe the effects of salmeterol and caffeine on this model. METHODS: The fatigued diaphragm model was set up with mixed gas including 21 %O2, 12 %CO2 and 67 % N2, and the effects of salmeterol (10-9-10-6mol·L-1) and caffeine (10-5-10-3 mol·L-1) on fatigued diaphragmatic contractility were surveyed. RESULTS: The value of force decreased by gas mixture at each frequency (5-120Hz).PTT, maintaining tension, and tension by muscle-stimulation decreased significantly; diaphragmatic contractile force of diaphargma increased by salmeterol with the concentration of 10-9-10-6mol·L-1at the frquencies 40-120 Hz(P <0.05);contractile force of diaphragma and PTT, maintaining tension and tension by muscle-stimulation significantly increased by caffeine with the concentration of 10-4 or 10-3mol·L-1 atall stimulus frequencies (5-120 Hz) (P <0.01). CONCLUSION: The model of rat diaphragmatic fatigue in vitro induced by hypoxic and hypercapnic acidosis can be set up by aerating with 3 kind of gas mixed with 21 % O2, 12 %CO2 and 67 %N2, and contractility of fatigued diaphragm of rat enhanced by salmeterol and caffeine to some extent.

Key words: salmeterol, caffeine, diaphragmatic fatigue, model

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