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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (7): 785-787.

• 研究原著 • 上一篇    下一篇

银杏叶提取物抗鼠肾缺血再灌注损伤及对P选择素表达的影响

张万帆, 杨吉乡1, 谢守霞2, 原文鹏2, 庞春平3, 张欣洲   

  1. 暨南大学医学院附属第二医院、深圳市人民医院肾内科, 1急诊科、 2药学部、 3病理科, 深圳518020, 广东
  • 收稿日期:2004-06-07 修回日期:2004-06-26 出版日期:2004-07-26 发布日期:2020-11-20
  • 通讯作者: 张万帆,男,硕士研究生,副主任医师,研究方向:肾脏病学临床与研究。Tel:0755-25533018-3522Email:szzwf2000@yahoo.com.cn
  • 基金资助:
    深圳市科技局基金资助项目(№199904060)

Effects of Ginkgo biloba extract on anti-ischemia reperfusion injury and PSelectin expression in rats kidney

ZHANG Wan-Fan, YANG Ji-Xiang1, XIE Shou-Xia2, YUAN Wen-Peng2, PANG Chun-Ping3, ZHANG Xin-Zhou   

  1. Department of Nephrology, 1Department of Emergency, 2Department of Pharmacy, 3Department of Pathology, Shenzhen People's Hospital, The Second Hospital of Jinan University, Shenzhen 518020, Guangdong, China
  • Received:2004-06-07 Revised:2004-06-26 Online:2004-07-26 Published:2020-11-20

摘要: 目的: 探讨银杏叶提取物保护大鼠肾脏缺血再灌注损伤作用与机理。方法: SD 大鼠随机分为假手术组、缺血再灌注组、银杏叶提取物组。通过HE染色观察肾组织损伤和中性粒细胞浸润,免疫组织化学方法观察P选择素的表达情况。结果: 与假手术组相比,缺血再灌注组肾小管上皮细胞呈明显的缺血性改变,P选择素表达明显增强(P<0.01),肾小球内中性粒细胞增加明显(P<0.001);银杏叶提取物组比缺血再灌注组肾小管上皮细胞缺血性改变减轻,P选择素表达减少(P<0.01),肾小球内中性粒细胞较缺血再灌注组减少(P<0.001)。结论: 银杏叶提取物减轻大鼠肾缺血再灌注损伤,减少P选择素表达及中性粒细胞浸润。

关键词: 银杏叶提取物, P 选择素, 缺血再灌注损伤, 肾脏, 中性粒细胞

Abstract: AIM: To investigate the effects of Ginkgo biloba extract on rats with renal ischemia reperfusion injury and its protective mechanism was discussed.METHODS: A model of renal ischemia reperfusion injury was set up.34 SD rats were randomly divided into sham operated group (n=10), ischemia reperfusion group (n=12), and Ginkgo biloba extract injection group (n=12).The renal ischemic injury and neutrophil infiltrate in tissue stained with hematoxylin and eosin were observed.The expression of P-Selectin in kidney was evaluated by immunohistochemistry.RESULTS: As compared with sham operated group, some ischemic changes increased significantly in the renal tubular epithelial cells, P-selectin expression (P<0.01)and neutrophils in glomerular increased in ischemia reperfusion group (P<0.001).As compared with ischemia reperfusion group, some mild ischemic changes alleviated significantly in the renal tubular epithelial cells and P-selectin expression (P<0.01)and neutrophils in glomerular decreased in Ginkgo biloba extract injection group (P<0.001).CONCLUSION: The administration of Ginkgo biloba extract can alleviate rat renal ischemia reperfusion injury, and decrease P-selectin expression and neutrophils infiltration in glomerular.

Key words: Ginkgo biloba extract, P-selectin, ischemia reperfusion injury, kidney, neutrophil

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