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中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (1): 40-43.

• 研究原著 • 上一篇    下一篇

复方白花前胡液对大脑中动脉缺血再灌注大鼠Caspase-3 蛋白表达的影响

董梦久, 涂晋文, 周晓莉   

  1. 湖北省中医院神经内科, 武汉 430060, 湖北
  • 收稿日期:2004-07-26 修回日期:2004-11-12 出版日期:2005-01-26 发布日期:2020-11-19
  • 通讯作者: 董梦久, 女, 博士, 主任医师, 教授, 研究方向:中医脑病, 中医急症。Tel:027-88929203 E-mail:dongmengjiu@sina.com

Effects of FFBHQH on level of expression of caspase-3 protein of middle cerebral artery in ischemia/reperfusion models

DONG Meng-jiu, TU Jin-wen, ZHOU Xiao-li   

  1. Department of Neurology, Hubei Hospital of Traditional Chinese Medicine, Wuhan 430060, Hubei, China
  • Received:2004-07-26 Revised:2004-11-12 Online:2005-01-26 Published:2020-11-19

摘要: 目的: 探索复方白花前胡液对大脑中动脉缺血再灌注大鼠Caspase-3 蛋白表达的影响。方法: 线栓法制备大脑中动脉缺血再灌注大鼠模型, 术后24、48、72 h 分别检测大鼠的神经功能缺损;并采用免疫组织化学法检测凋亡相关基因Caspase-3 蛋白表达。结果: MCAO/R 24、48、72 h 后, 所有动物都出现程度不同的运动障碍, 且大鼠脑组织中Caspase-3蛋白表达显著升高, 复方白花前胡液可明显改善大脑中动脉缺血再灌注大鼠的神经功能缺损症状, 抑制大鼠Caspase-3 蛋白表达水平。结论: 复方白花前胡液能有效抑制凋亡相关基因Caspase-3 蛋白的表达, 减轻或消除缺血级联反应中迟发性神经元死亡,复方白花前胡液是其治疗缺血性中风病的机理之一。

关键词: 复方白花前胡液, 大脑中动脉缺血再灌注, 大鼠, 凋亡相关基因

Abstract: AIM: To investigate the effects of FFBHQH on the level of expression of caspase-3 protein of middle cerebral artery in ischemia reperfusion model and to study its mechanisms in preventing and treating ischemic apoplexy.METHODS: The model of focal cerebral ischemia reperfusion in rats was established with the suture-occluded method.The effects of FFBHQH on the behavior obstacle of rats after MCAO/R 24, 48, and 72 h were observed, and the levels of expression of caspase-3 protein were observed by immunoassay method in rats treated with FFBHQH after the cerebral ischemia reperfusion.RESULTS: Different degrees of behavior obstacle appeared in rats after MCAO/R 24, 48, 72 h, and FFBHQH reduced the behavior obstacle grade.After MCAO/R, the expression of caspase-3 protein increased obviously, but FFBHQH reduced the expression.CONCLUSION: FFBHQH can prevent and treat the ischemic apoplexy, and the mechanisms may relate to the calcium antagonism and the prevention of the apoptosis correlated gene such as caspase-3 after cerebral ischemia.

Key words: cerebral infarction, ischemia reperfusion, FFBHQH, rats, caspase-3

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