海狗油保肝作用的实验研究

摘要/Abstract

摘要： 目的: 观察海狗油对化学毒物所致小鼠、大鼠急性肝损伤及对长期喂食高脂饲料大鼠的影响。方法: 实验分为6 组(n = 10):即空白组、模型组、阳性药组、海狗油低、中、高剂量组。化学毒物乙硫氨酸或四氯化碳致急性肝损伤实验:小鼠和大鼠各组分别灌胃(ig)给供试药9d或7d;d 7 ig DL-乙硫氨酸,实验结束时测定肝脂:d6 ip四氯化碳,实验结束时测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和组织学检查。长期喂食高脂饲料大鼠实验:于实验d1 一次性皮下注射小剂量四氯化石桌并长期喂食高脂饲料造成脂肪肝模型大鼠,同时各组分别ig供试药, 每天1次,连续10周后,称肝脏重量并计算肝脏系数,测定血脂、肝脂、脂质过氧化指标和组织学检查。结果: 对乙硫氨酸致急性肝损伤的小鼠和大鼠ig 给予海狗油(小鼠7.2g·kg^-1,大鼠4.8g·kg^-1)后,与模型组比较甘油三酣(TG)分别降低23%和16%(P < 0. 01);对四氯化碳致急性肝损伤的大鼠,海狗油4. 8 mg·kg^-1组使ALT降低24%(P<0.01)、AST降低16%(P < 0.01)。长期喂食高脂饲料大鼠,与模型组比较,海狗油 4.8mg·kg^-1组血清总胆固醇(TC)、游离脂肪酸(FFA)、肝重量及肝细胞内 TG、FFA 和丙二醒(MDA)含量显著降低(P < 0.01), 超氧化物歧化酶(SOD)活性升高(P<0.01),组织学检查显示肝细胞内脂变程度减轻,脂滴减少。结论: 海狗油对化学毒物所致急性肝损伤的肝细胞有保护作用,对脂肪肝的形成具有明显的预防作用。

关键词: 海狗油, 肝保护, 肝损伤, 脂肪肝, 肝脂

Abstract: AIM: To study the effect of seal oil for protecting liver cells from acutely damaged by chemicals or chronically damaged by feeding with high fat diet in mice or rats.METHODS: KM mice and Wistar rats were divided randomly by body weight to 6 groups (n = 10 in each including placebo control group received only oliveil, model group received olive oil too, low or medial orlarge dose of seal oil groups received seal oil, control drgroup received dimethyl-4, 4'-dimethoxy-5, 6, 5',6'-dimethylenedioxybiphenyl-2, 2' dicarboxy late or simvastatin for 9, 7 days or 10 weeks. Except for placebo control group the mice or rats were acutely damaged by Dir ethionine or carbon tetrachloride on 7 or 6th day. The content of triglyeeride (TG) in liver of both mice and rats or se-rum transaminase ALT, and AST of rats were tested in the end. The rats were chronically damaged byntaneous injecting a low dose of carbon tetrachloride on the first day and feeding with high fat diet for 10 weeks. The lipid levels in both serum and liver and the hepatic weight were pathology was evaluated.RESULTS: while the mice and rats were damaged by DL. ethionine, the content of TG was 23% and 16% lower than model group(P < 0. 01) in the mice Of 7.2 g·kg^-1 seal oil groupand in the rats of 4.8 g·kg^-1 seal oil group, respectively. In damaged rats by carbon tetrachloride, serum ALTand AST decreased 24% and 16% comparing with modelgroup (P < 0.01) in 4.8 gkg seal oil group. Inchronically damaged rats by feeding with high fat diet, thetotal cholesterol (TC) and free fatty acid (FFA) in serum, TG, TC, FFA, malonyldialdehyde (MDA) in liverand hepatic weight decreased significantly than modelgroup (P < 0.01) and superoxide dismutase (SOD) inliver increased significantly than model group (P < 0. 01)4.8 gkg seal oil group. Pathologic histomorphologyinvestigation showed hepatocytes with lipid droplet decreased than model group in seal oil groups.CONCLUSION: Seal oil can protect liver cells from acutely damaged by DI-ethionine or carbon tetrachloride and significant prevention effect on fatty liver.

Key words: seal oil, protect liver, acutely dam-aged, fatty liver, liver lipid

中图分类号:

R965.1

李湛军, 乐嘉静, 徐康森. 海狗油保肝作用的实验研究[J]. 中国临床药理学与治疗学, 2005, 10(10): 1135-1139.

LI Zhan-jun, LE Jia-jing, XU Kang-sen. Effect of seal oil for protecting liver cells from acutely or chronically dam aged in mice and rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(10): 1135-1139.

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海狗油保肝作用的实验研究

Effect of seal oil for protecting liver cells from acutely or chronically dam aged in mice and rats

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