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中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (2): 191-195.

• 研究原著 • 上一篇    下一篇

大黄素对体外大鼠骨髓基质细胞向成骨细胞方向分化的影响

刘钰瑜, 姚卫民1, 艾春媚2, 许碧连, 邹丽宜, 崔燎, 吴铁   

  1. 广东医学院药理学教研室, 1呼吸疾病研究所,2广东天然药物研究与开发重点实验室, 湛江524023, 广东
  • 收稿日期:2004-10-13 修回日期:2004-11-21 出版日期:2005-02-06 发布日期:2020-11-18
  • 通讯作者: 崔燎, 女, 教授, 硕士生导师, 主要研究方向:防治骨质疏松药物研究。Tel:0759-2388405 E-mail:cui liao@yahoo.com
  • 作者简介:刘钰瑜, 女, 讲师, 硕士。Tel:0759-2388588 E-mail:Liuyuyu22@yahoo.com
  • 基金资助:
    广东医学院青年基金资助(NoXQ0307)

Effects of emodin on differentiation of bone marrow stroma cell into osteoblast in rats in vitro

LIU Yu-yu, YAO Wei-min1, AI Chun-mei2, XU Bi-lian, ZOU Li-yi, CUI Liao, WU Tie   

  1. Department of Pharmacology, 1Institune of Respiratory Disease, 2Guangdong Key Laboratory For Research And Development of Natural Drugs, Guangdong Medical College, Zhanjiang 524000, Guangdong, China
  • Received:2004-10-13 Revised:2004-11-21 Online:2005-02-06 Published:2020-11-18

摘要: 目的:观察大黄素对骨髓基质细胞向成骨细胞方向分化的影响, 探讨大黄素对干细胞分化方向的调节作用。方法:用密度梯度离心的方法和传代贴壁筛选的方法相结合, 分离纯化骨髓基质细胞。对硝基苯磷酸盐法判断是否成功诱导骨髓基质细胞向成骨细胞方向分化, 碱性磷酸酶染色法、放射免疫测定法观察大黄素对骨髓基质细胞向成骨细胞方向分化的影响。结果:结合密度梯度离心的方法和传代贴壁筛选的方法, 分离纯化后可得到成分较为均一的骨髓基质细胞, 其间存在有间质干细胞, 经诱导后向成骨细胞方向分化。大黄素可增加大鼠骨髓基质细胞成骨诱导后细胞碱性磷酸酶的含量, 其中10-6 mol°L-1浓度组作用7 d 时效果明显。大黄素在2.5×10-7~10-5 mol°L-1浓度范围内未能增加细胞上清液和细胞裂解液中骨钙素含量。结论:大黄素能促进骨髓基质细胞向成骨细胞方向分化, 这一作用主要体现在骨形成过程的早期, 对矿化期没有促进作用。

关键词: 大黄素, 骨髓基质细胞, 成骨细胞, 分化, 大鼠

Abstract: AIM: To investigate the effects of emodin on the differentiation of MSCs into osteoblast in rats in vitro.METHODS: The MSCs were isolated from onemonth old SD rats by density-gradient centrifugation and the ALP activity and osteocalcin content were examined by PNPP and radioimmunoassay.RESULTS: Through density-gradient centrifugation isolation and subculture attachment selection, the cultured MSCs became much purified.Emodin induced the differentiation of MSCs into osteoblast by increasing ALP activity.No significant effects of emodin on osteocalcin content of MSCs were observed at concentration from 2.5×10-7 to 10-5 mol°L-1. CONCLUSION: Emodin can partly induce the osteogenic differentiation.

Key words: emodin, bone marrow stromal cells, osteoblast, differentiation, rat

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