硫喷妥钠或异丙酚麻醉不同时期大鼠脑ATP 酶和NO 合酶活性以及NO 生成量的动态变化

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (3): 265-269.

硫喷妥钠或异丙酚麻醉不同时期大鼠脑ATP 酶和NO 合酶活性以及NO 生成量的动态变化

刘红亮, 戴体俊¹, 姚尚龙

华中科技大学同济医学院协和医院麻醉科, 武汉430030, 湖北;
¹徐州医学院麻醉药理教研室, 徐州221002, 江苏

收稿日期:2004-10-30 修回日期:2004-12-13 出版日期:2005-03-26 发布日期:2020-11-18

Dynamic changes of ATPases and NOS activities and NO production at different anesthesia phases of thiopental and propofol anesthesia

LIU Hong-liang, DAI Ti-jun¹, YAO Shang-long

Department of Anesthesiology, Union Hospital Tongji Medical College, Huazhong Technology University, Wuhan 430030, Hubei, China;
¹Department of Anesthesia Pharmacology, Xuzhou Medical College, Xuzhou 221002, Jiangsu, China

Received:2004-10-30 Revised:2004-12-13 Online:2005-03-26 Published:2020-11-18
Contact: LIU Hong-liang, male, medical doctor, engaged in the study of mechanisms and effects of general anesthesia.Tel:027-62535517 E-mail:liuhl75@163.com
About author:DAI Ti-jun, male, professor, advisor of master, engaged in anesthesia pharmacology. YAO Shang-long, male, professor, advi sor of doctor, engaged in the study of mechnisms and effect s of general anesthesia
Supported by:
Supported by National Natural S cience Fund of China (№39970715)

摘要/Abstract

摘要： 目的:观察硫喷妥钠或异丙酚麻醉不同时期,大鼠皮层、脑干Ca²⁺-ATPase、Na⁺, K⁺-ATPase 活性、NOS 活性和NO 生成量的动态变化。方法:大鼠腹腔注射硫喷妥钠30 mg°kg^-1 或异丙酚100 mg°kg^-1,以翻正反射消失为标准, 分别于诱导期、麻醉期, 恢复期和苏醒期断头取脑(n=8), 分离出皮层和脑干, 测定Ca²⁺-ATPase、Na⁺, K⁺-ATPase、NOS 的活性以及NO 的生成量, 腹腔注射生理盐水10 ml°kg^-1作为对照组。结果:大鼠皮层、脑干Ca²⁺-ATPase、Na⁺, K⁺-ATPase 的活性在硫喷妥钠诱导期、麻醉期和恢复期显著性降低(P<0.05 或P<0.01), 在异丙酚麻醉期亦显著性降低(P<0.05)。腹腔注射硫喷妥钠或异丙酚后, NOS 活性和NO 生成量从诱导期到恢复期均显著性降低(P<0.01), 在苏醒期可恢复至对照水平。结论:Ca²⁺-ATPase、Na⁺, K⁺-ATPase、NOS 活性及NO 的产生介导了硫喷妥钠或异丙酚的麻醉作用。

关键词: ATP酶, NO合酶, NO, 硫喷妥钠, 异丙酚

Abstract: AIM: To investigate the dynamic changes of ATPases and NOS activities and NO production at different anesthesia phases using thiopental and propofol anesthesia.METHODS: Thiopental of 30 mg°kg^-1 or propofol of 100 mg°kg^-1 was administered respectively at different anesthetic phases (induction, anesthesia, restoration, and awake), the activities of NOS and ATPase and NO production in cortex and brain stem were measured.Saline 10 ml°kg^-1 was injected as the control group.RESULTS: Ca²⁺-ATPase and Na⁺, K⁺-ATPase activities in the cortex and brain stem were significantly decreased after administration of thiopental and propofol, especially at induction, anesthesia, or even restoration phase of thiopental group (P<0.05, P<0.01) and at anesthesia phase of propofol group (P<0.05).NOS activities and NO production decreased from induction to restoration phase with thiopental and propofol anesthesia (P<0.01).The parameters were returned near to the normal at awaken phase.CONCLUSION: Activities of ATPases and NOS and the production of NO may mediate the anesthesia effects of thiopental and propofol in the rat cortex and brain stem.

Key words: ATPase, nitric oxide synthase, nitricoxide, thiopental, propofol

中图分类号:

R966
R971.2

刘红亮, 戴体俊, 姚尚龙. 硫喷妥钠或异丙酚麻醉不同时期大鼠脑ATP 酶和NO 合酶活性以及NO 生成量的动态变化[J]. 中国临床药理学与治疗学, 2005, 10(3): 265-269.

LIU Hong-liang, DAI Ti-jun, YAO Shang-long. Dynamic changes of ATPases and NOS activities and NO production at different anesthesia phases of thiopental and propofol anesthesia[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(3): 265-269.

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