石杉碱甲对大鼠海马脑片 CA1 神经元 θ节律及长时程增强的影响

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (5): 494-498.

石杉碱甲对大鼠海马脑片 CA1 神经元 θ节律及长时程增强的影响

吴小未^1,2, 汪萌芽¹

¹皖南医学院细胞电生理研究室, 芜湖 241001, 安徽;
²首都医科大学附属复兴医院神经内科, 北京 100038

收稿日期:2005-04-05 修回日期:2005-05-08 出版日期:2005-05-26 发布日期:2020-11-19
通讯作者: 汪萌芽,男, 博士, 教授, 硕士生导师, 主要从事神经细胞电生理和电药理学研究。Tel:0553-3815565 E-mail: wangmy @mail.wh.ah.cn
作者简介:吴小未, 女, 硕士, 主治医师, 主要从事神经疾病的临床和基础研究。
基金资助:
国家自然科学基金项目(No30270366); 安徽省人事厅人才专项基金(No2003)

Effects of huperzine A on theta-rhythm and long-term potentiation in CA1 pyramidal neurons in adult rat hippocampal slices

WU Xiao-wei^1,2, WANG Meng-ya¹

¹Cell Electrophysiology Lab, Wannan Medical College, Wuhu241001, Anhui, China;
²Department of Neurology, Fux-ing Hospital, Capital Medical University, Beijing 100038, China

Received:2005-04-05 Revised:2005-05-08 Online:2005-05-26 Published:2020-11-19

摘要/Abstract

摘要： 目的: 研究石杉碱甲(Hup-A) 对大鼠海马脑片CA1锥体神经元θ节律、长时程增强的影响, 以分析其增强学习记忆功能的神经细胞电生理机制。方法: 应用大鼠海马脑片神经元细胞内记录技术, 观察石杉碱甲对大鼠海马脑片的 CA1 锥体神经元 θ节律、长时程增强的影响。结果: (1) 未用药组出现膜电位振荡前后 4~ 10 Hz(θ节律) 功率分量之和无显著性差异, 但在 Hup-A(1 μmol°L^-1) 灌流 15 min 后出现膜电位振荡, 与用药前没有膜电位振荡的 4 ~10 Hz 功率分量之和配对 t 检验比较有显著差异 (n=3, P<0.005)。(2) 在 LTP 期间, 对照组 EPSP 幅度在强直刺激后 30 min 显著性的升高(P<0.05), 而Hup-A 组在强直刺激后 15 min 即出现显著性升高(P<0.01)。结论: Hup-A 可增加大海马锥体神经元在θ频率范围内的功率分量, 并易化 LTP 的诱发, 这可能是其增强学习记忆功能的细胞电生理机制之一。

关键词: 大鼠, 海马脑片, 锥体神经元, θ节律, 长时程增强, 石杉碱甲

Abstract: AIM: To study the effects of huperzine A on the theta-rhythm and long-term potentiation in CA1 py-ramidal neurons in adult rat hippocampal slices and to get insight into the cell electrophysiological mechanisms un-derlying the potentiation of learning and memory by Hu-perzine A.METHODS: The intracellular recordings from CA1 pyramidal neurons in hippocampal slices related to learning and memory were made to analyze the mecha-nisms of Hup-A action.RESULTS: Power of frequencies ranged from 4 to 10Hz wasnot significantly difference be-tween pre-membrane potential oscillations (MPOs) and post-MPOs in control group, but it was significantly (P<0.005, n =3) changed between pre-MPOs before ad-dition of Hup-A and post-MPOs after addition of Hup-A. During long-term potentiation (LTP), the increase of am-plitudes of EPSPs was observed at post-tetanus 30 min in control group and 15 min in Hup-A group.CONCLUSION: Hup-A can increase the power of 4 to 10 Hz MPOs and facilitate induction of LTP in CA1 pyramidal neurons of rat hippocampal slices, which may contribute to the potentiation of learning and memory by Huperzine A.

Key words: rats, hippocampal slices, pyramidal neuron, theta-rhythm, long-term potentiation (LTP), hu-perzine A

中图分类号:

R971.91

吴小未, 汪萌芽. 石杉碱甲对大鼠海马脑片 CA1 神经元 θ节律及长时程增强的影响[J]. 中国临床药理学与治疗学, 2005, 10(5): 494-498.

WU Xiao-wei, WANG Meng-ya. Effects of huperzine A on theta-rhythm and long-term potentiation in CA1 pyramidal neurons in adult rat hippocampal slices[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(5): 494-498.

参考文献

1 梅镇彤.学习与记忆[A]. 见:韩济生, 主编.神经科学原理[M]. 北京:北京医科大学出版社, 1999:916-35
2 Rose GM, Dunwiddie TV.Induction of hippocampal long-term potentiation using physiologically patterned stimulation [J]. Neurosci Lett, 1986;69:244-8
3 Hölscher C, Anwyl R, Rowan MJ.Stimulation on the positive phase of hippocampal theta rhythm induces long-term potentia-tion that can be depotentiated by stimulation on the negative phase in area CA1 invivo[J]. J Neurosci, 1997;17:6470-7
4 管林初, 陈双双, 崔秋耕, 陆维华, 唐希灿.石杉碱甲对动物行为和脑皮质电图的影响[J]. 心理学报, 1991;23:404-11
5 汪萌芽.大鼠海马脑片锥体神经元的细胞内记录[J]. 皖南医学院学报, 1992;11:235-8
6 Bland BH, Colom LV, Konopacki J, Roth SH.Intracellular records of carbachol-induced theta rhythm in hippocampal slices [J]. Brain Res, 1988;447:364-8
7 Konopacki J, Bland BH, MacIver MB, Roth SH.Cholinergic theta rhythm in transected hippocampal slices:independent CA1 and dentate generators[J]. Brain Res, 1987;436:217-22
8 Buzsáki G, Draguhn A.Neuronal oscillations in cortical net-works[J]. Science, 2004;304:1926-9
9 Leung LS, Yim CC.Intrinsic membrane potential oscillations in hippocampal neurons in vitro[J]. Brain Res, 1991;553:261-74
10 Larson J, Lynch G.Induction of synaptic potentiation in hipp-ocampus by patterned stimulation involves two events[J]. Sci-ence, 1986;232:985-8
11 Xu C, Datta S, WuM, Alreja M.Hippocampal theta rhythm is reduced by suppression of the H-current in septohippocampal GABAergic neurons[J]. Eur J Neurosci, 2004;19:2299-309
12 Hyman JM, Wyble BP, Goyal V, Rossic A, Hosselmo ME. Stimulation in hippocampal region CA1 in behaving rats yields long-term potentiation when delivered to the peak of theta and long-term depression when delivered to the trough[J]. J Neu-rosci, 2003;23:11725-31
13 Bliss TVP, Collingridge GL.A synaptic model ofmemory:long-term potentiation in the hippocampus[J]. Nature, 1993;361: 31-9
14 Barrionuevo G, Kelso SR, Johnston D, Brown TH.Conduc-tance mechanism responsible for long-term potentiation inmono-synaptic and isolated excitatory synaptic inputs to hippocampus [J]. JNeurophysiol, 1986;55:540-50
15 Stevens CF, Wang Y.Changes in reliability of synaptic function as a mechanism for plasticity [J]. Nature, 1994;371:704-7

[1]	许秀荷, 何晓丽, 周佳顺, 潘丽珠, 周卓君, 李佳悦, 王彩霞, 袁伟, 朱桂琦. 蜂蜜对大鼠血尿酸水平及肾损伤研究[J]. 中国临床药理学与治疗学, 2023, 28(7): 743-750.
[2]	汪东辉, 蒋素文, 胡爱荣, 朱波, 何哲耘, 张露侃, 王家岚, 范莹, 林恳. 桑葚改善大鼠非酒精性脂肪性肝病的氧化应激损伤[J]. 中国临床药理学与治疗学, 2023, 28(6): 609-616.
[3]	李红琴, 曹利民, 金满意, 王建龙, 柴晓萍, 田苗苗. 牵正散不同剂型改善大鼠面神经损伤作用研究[J]. 中国临床药理学与治疗学, 2023, 28(6): 617-623.
[4]	衡冰冰, 杨丹峰, 戴舒阳, 上官王宁. 丙泊酚对皮下注射氯喹所致瘙痒大鼠脊髓TRPV1和CB1受体的影响[J]. 中国临床药理学与治疗学, 2023, 28(10): 1101-1108.
[5]	张静, 高丹阳, 于康, 林晓婉, 曹颖, 刘晓, 缪慧慧, 李天佐. 不同吸入麻醉药对新生期大鼠神经胶质细胞活化的影响[J]. 中国临床药理学与治疗学, 2022, 27(12): 1391-1399.
[6]	张远金, 黄盛博, 刘洁, 王昕. 药物代谢创新模型：基因编辑大鼠和小肠3D类器官[J]. 中国临床药理学与治疗学, 2021, 26(8): 914-922.
[7]	王香溢, 盛洁, 王秀, 郭岩, 陈志武. UPLC-MS/MS法测定氘代氯化琥珀胆碱在大鼠体内分布[J]. 中国临床药理学与治疗学, 2021, 26(3): 271-277.
[8]	杨慧芳, 罗颖, 赵莉. 右美托咪定对脓毒症大鼠肾功能及血清炎症因子的影响[J]. 中国临床药理学与治疗学, 2020, 25(8): 857-861.
[9]	张俊秀, 沈媛媛, 杨解人, 郑书国, 郝伟. 联用芝麻素和维生素E抑制PI3K/AKT信号通路改善自发性高血压大鼠的左室纤维化[J]. 中国临床药理学与治疗学, 2020, 25(11): 1214-1222.
[10]	衡冰冰，戴舒阳，杨丹峰，Beekoo Deepti，连庆泉，上官王宁. 丙泊酚对吗啡鞘内注射所致瘙痒大鼠脊髓内胃泌素释放肽受体的影响[J]. 中国临床药理学与治疗学, 2019, 24(7): 759-765.
[11]	高艺文，张楠，吕婵梅，杨宣梅，岳桂华. 黄连解毒汤对幼龄自发性高血压大鼠血管内皮NO通路影响的研究[J]. 中国临床药理学与治疗学, 2019, 24(3): 248-253.
[12]	赵韶静，曾艳，李正东，张华. 左卡尼汀对大鼠糖尿病肾病的保护作用分析[J]. 中国临床药理学与治疗学, 2019, 24(3): 278-282.
[13]	杜天平，孟祖东. 17β雌二醇对异丙酚诱导发育期大鼠海马神经细胞凋亡时p38MAPK信号传导通路的作用机制[J]. 中国临床药理学与治疗学, 2019, 24(2): 128-133.
[14]	张恒，吴海啸，胡洋，杨庆，黄汀. 车前子提取物通过调控PI3K/AKT通路改善糖尿病大鼠神经源性膀胱病的作用研究[J]. 中国临床药理学与治疗学, 2019, 24(11): 1249-1255.
[15]	谢方英，陈浙丽，高月蓝. 益气养阴生肌方对复发性口腔溃疡大鼠的治疗作用及其对黏膜炎症因子和机体免疫功能的影响[J]. 中国临床药理学与治疗学, 2019, 24(1): 20-26.