欢迎访问《中国临床药理学与治疗学》杂志官方网站,今天是

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (5): 532-536.

• 研究原著 • 上一篇    下一篇

3, 4,5, 6-四羟基 口山 酮对大鼠离体心肌缺血-再灌注损伤的保护作用

戴忠, 胡长平1, 吴铁, 戴滨, 林熙   

  1. 广东医学院药理教研室, 湛江 524023, 广东;
    1中南大学药学院药理学系, 长沙 410078, 湖南
  • 收稿日期:2005-03-09 修回日期:2005-04-25 出版日期:2005-05-26 发布日期:2020-11-19
  • 通讯作者: 戴忠,男, 医学博士, 副教授, 研究方向:心血管药理学。Tel:0759-2388588-3 E-mail:daizhong dai@yahoo.com.cn

Effects of 3,4,5, 6-tetrahydroxyxanthone on myocardial ischemia-reperfu-sion injury in isolated rat hearts

DAI Zhong, HU Chang-ping1, WU Tie, DAI Bin, LIN Xi   

  1. Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong, China;
    1Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha410078, Hunan, China
  • Received:2005-03-09 Revised:2005-04-25 Online:2005-05-26 Published:2020-11-19

摘要: 目的: 研究合成的 口山 酮化合物 3, 4, 5, 6-四羟基 口山 酮对离体大鼠心肌缺血-再灌注损伤的保护作用及其机制。方法: 离体大鼠心脏采用 Langendorff法灌流, 停灌 30 min 再灌 30 min 造成心肌缺血-再灌注损伤模型。左心室插入水囊导管, 记录左室内压(LVP)、左室内压最大上升速率(+dp/dtmax) 和心率(HR), 定时收集冠脉流出液, 测定冠脉流量(CF)和肌酸激酶(CK) 活性。心脏灌流结束后心脏称重,计算单位心脏湿重的 CK 释放量。心肌组织制备匀浆, 用放射免疫法测定心肌组织 TNF-α含量。结果: 预先 给予 3, 4, 5, 6-四 羟基 口山 酮(30、100 或 300Μmol°L-1) 可显著改善缺血-再灌注所致的心功能损伤, 减少CK 的释放和心肌组织 TNF-α的产生。结论: 3, 4, 5, 6-四羟基 口山 酮对心肌缺血-再灌注损伤具有保护作用, 其作用机制可能与抑制 TNF-α的产生有关。

关键词: 3,4,5,6-四羟基 口山 酮, 大鼠, 心肌缺血-再灌注, 肿瘤坏死因子-α

Abstract: AIM: To study the protective effects and mechanisms of 3, 4, 5, 6-tetrahydroxyxanthone on myocar-dial ischemia-reperfusion injury in isolated rat hearts. METHODS: Ischemia-reperfusion injury was induced by 30 min of global ischemia and 30 min of reperfusion in isolated rat hearts.Heart rate, coronary flow (CF), left ventricular pressure (LVP), and the first derivative (+ dp/dtmax) were recorded.The activity of creatine kinase in coronary effluent and tumor necrosis factor-alpha (TNF-α) content in myocardial tissues were measured. RESULTS: 3, 4, 5, 6-tetrahydroxyxanthone (30, 100 or 300 μmol°L-1) caused a significant improvement of car-diac function and a decrease in the release of creatine ki-nase in coronary effluent as well as the level of TNF-αin myocardial tissues.CONCLUSION: 3, 4, 5, 6-tetra-hydroxyxanthone possesses a protective effect onmyocardi-al ischemia-reperfusion injury, and the effects may be re-lated to inhibition of TNF-αproduction.

Key words: 3,4,5,6-tetrahydroxyxanthone, myo-cardial ischemia-reperfusion, tumor necrosis factor-alpha

中图分类号: